筛选

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

The traffic sensor data screening of intelligent transportation system was discussed and the data flow exchange between data screening unit and other system of Traffic Information Center was described. The content and general procedure of data screening were introduced. The checking condition of data reasonableness and consistency in different traffic conditions were analysized.

对ITS中交通传感器数据的筛选问题进行了探讨，描述了数据筛选单元与其它系统间数据流的交换情况，介绍了数据筛选的内容和一般过程，并详细分析了不同交通条件下，不同交通传感器数据的合理性检验和一致性检验条件。

Repens L. and E. intermedia Nevski by using SSR, in order to provide science theories basis and base to further study E. repens L. drought resistance gene molecular marker, discover excellent drought resistance gene source , fast and efficiently select with the molecular marker of drought resistance gene catena for E.

通过对20 份来源不同的偃麦草与中间偃麦草种质材料在室内模拟逆境和田间干旱条件下进行抗旱材料的筛选和鉴定，应用SSR 技术对偃麦草抗旱分子标记进行引物筛选，为进一步深入研究偃麦草与中间偃麦草抗旱基因分子标记、发掘优异抗旱基因源，快速高效筛选出与偃麦草与中间偃麦草抗旱基因连锁的分子标记提供科学理论依据和基础。

Can also be set up in high concentration hydraulic pulper, from the high concentration of water in the pulper out of waste pulp for coarse screening to remove large, heavy debris to reduce the flow back of the burden of screening equipment, large-scale paper machine And high output device of choice.

用于对废纸浆进行粗大杂质的筛选，可设置于高浓水力碎浆机之后，对从高浓水力碎浆机中出来的废纸浆进行粗筛选，除去大、重、杂物，减轻后面流程中筛选设备的负担，是大型纸机，高产量的首选设备。

Dr. Tilson identified several barriers to screening in private practice."One barrier is actually parent refusal," she told Medscape."Some parents don't want to acknowledge that their child is overweight or they aren't interested in any lifestyle interventions." She also cited time pressure on physicians."The screening tool we have put together is to make as easy as possible in a busy clinic," Dr. Tilson said.

Tilson医师认为在筛选时有一些障碍，她向Medscape表示，其中一个障碍是父母拒绝；有些父母不愿承认他们的孩子是过胖的，并且对於生活型态介入没有兴趣；Tilson医师表示，她也表示医师有时间上的压力；这项筛选工具清单使得筛选可以在繁忙的诊间中进行。

To provide a list of screening, the growth rate of screening, screening contrast, vertical analysis, technical analysis and other methods.

提供列表筛选、增长率筛选、对比筛选、纵向分析、技术分析等多种方法。

Rice saccharification medium as selective medium for Acetobacter, easy to operate and with good effect, is better than other selective mediums.

结果表明，以大米糖化汁培养基作为醋酸菌的筛选培养基，易操作，筛选效果好，优于其它的醋酸菌筛选培养基。

In this paper a new contingency screening and ranking method is formed by combiningiterative filtering screening technology with continuation power flow analysis. Researchingpreventive control policy in reactive power compensation and load control when themargin of stability is serious, and reciprocal math model is given;through the exempleof IEEE39 typical system , the availability of the discussed methods in this dissertationwas approved. Aimed at the real grid, the contingency screening analysis and preventivecontrol policy based on Beijing power grid is discussed;the static voltage stabilityanalysis software based on figure draw of Beijing grid is realized, and the software hasthe junction to BPA .

论文提出了将迭代过滤筛选技术和连续潮流法相结合的事故筛选分析方法；从无功补偿控制和负荷控制两方面研究了系统裕度紧张时的预防控制策略，给出了相应的数学模型；采用IEEE39节点的标准系统算例，验证了本文提出的静态电压稳定事故筛选分析方法的可行性；针对实际电网讨论了基于北京电网的事故筛选分析和预防控制策略；通过与BPA接口实现了基于北京电网图形平台的静态电压稳定分析软件。

The principal researches were as follow.(1) The lymphocyte of splenice and the head kidney of Siniperca were obtained by the tissue cultural method.

采用正负筛选方法，利用筛选药物G418和GCV筛选转染后的细胞筛选两周后，对存活细胞进行DNA水平和RNA水平鉴定。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。