筛选

To establish a new method to isolate genomic regulatory sequence using unpurified nuclear extract, here a new strategy was developed based on modified whole genome PCR and electrophoresis mobility shift assay. The principle is as follows. After sonication and ligation with DNA linker, human genomic DNA is amplified and labeled with isotope by whole genome PCR.

为了建立一种利用未经纯化的细胞核粗提物就能从人基因组中快速、大量筛选DNA调节片段的新方法，本研究对原有的全基因组PCR技术进行改进，结合凝胶阻滞实验，建立了筛选DNA调节片段的新方法。

The same is true for Go "When you're deciding what variations to consider, your subconscious mind is pruning," he said It's hard to say how much is going on in your mind to accomplish this pruning, but in a position on the board where I'd look at 10 varia

当你考虑棋局的种种变数时，你下意识地进行筛选"，巴布说，"很难说得清这个筛选过程是怎样的，我面对棋盘思考10种可能的变化情况，要得出相同的结论电脑却需要考虑上千种，甚至是上百万种的位置变化，而出来的结果还可能是错误的。

Dr. Tilson and colleagues developed a screening checklist tool based on the American Academy of Pediatrics and American Diabetes Association guidelines with some modifications. Participating physicians were to screen all children aged 10 to 18 years for elevated BMI. Children with a BMI higher than the 85th percentile were assessed for the risk factors of race/ethnicity, family history of type 2 diabetes, acanthosis nigrans, and hypertension (defined as systolic blood pressure higher than the 90th percentile for age and height).

Tilson医师与同事根据美国小儿医学会与美国糖尿病医学会的治疗指引进行部份修改，研发了一种核对清单；参与的执业医师筛选所有BMI过高的10至18岁儿童，BMI高於前15%的儿童接受筛选种族信仰、第二型糖尿病家族史、棘状黑色素瘤与高血压（以高於同年龄与身高前10%的收缩压来定义）。

Through screening lipases, lipase Novozym 435 and lipase Alcaligenes sp were found to show higher activity and better enantioselectivity for the resolution of 2-(2-chloro- 1-hydroxyethyl)thiophene. The optimal reaction conditions were finally achieved by further investigating the effects of solvents, temperature, rotation speed and enzyme concentrations.

通过对不同来源酶的筛选，找到了Novozym 435和Alcaligenes sp两种选择性较好的酶，它们均对该反应具有较高的选择性和较快的反应速度，在此基础上进一步通过对溶剂、温度、摇床转速以及酶用量的筛选，确定了能够有效拆分2-氯-1-(2-噻吩)-乙醇的较佳反应条件。

In the four placebo-controlled studies evaluating radiofrequency denervation outcomes in patients with "confirmed"l-z joint pain, the only study that screened patients with MBB demonstrated positive outcomes,157versus only one of three that used diagnostic intraarticularin jections.158–160 Ultrasound-guided medial branchand intraarticular blocks have also been demonstrated to provide comparable accuracy to fluoroscopically guided injections, although they may be less likely to detect low-volume intravascular uptake and are less accurate in obese patients.161–164 Because MBB are technically easier to perform than intraarticular injections and involve anesthetization of the nerves to be lesioned, it seems more logical to use these blocks as a prognostic tool before radiofrequency denervation.

在四个设有安慰剂对照的对于&确诊&小关节疼痛的患者行射频神经消融后的结果的研究中，唯一一个用MBB筛选研究对象的实验表现出阳性结果，而其他3个用关节内注射筛选的实验中只有1个表现出阳性。超声介导下的内侧支关节囊内阻滞被证实较透视介导更精确，虽然其血管内吸收剂量较低且在肥胖的患者中精确性较差。由于MBB较之关节内注射技术上更简单，且能对受损神经进行麻醉，利用这种阻滞作为神经射频消融前的诊断方法较为合理。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

In addition, the clinical data of 96 FAP cases clinically diagnosed between 1985 and 2000 were analyzed retrospectively, which included clinical (enteral and apenteric manifestations, methods of diagnosis and screening, surgical modality and follow-up findings).

我们认为结肠镜是FAP最可靠、最有效的临床诊断方法并在家系成员筛选中可发挥重要作用；CHRPE是具有高度特异性和敏感性的辅助诊断方式，可作为对未成年家系成员筛选的首选方法；FAP常伴发上消化道病变包括胃窦部增生性息肉，十二指肠腺瘤发病率高，但癌变少见；腹腔硬纤维瘤是FAP的严重结肠外病变，术后复发率高；FAP术后如直肠粘膜有残留，其腺瘤复发及癌变危险性高，应定期结肠镜随访。

Use tertiary system method to screen and optimize JLNCAO substrate, and use heat and dry under atmospheric pressure method, ignition residue method, The Casparian law of trace nitrogen, acid...

运用三级系统筛选法对菌草培养基进行筛选优化；用常压加热干燥法、灼烧法、微量凯氏定氮法、酸性洗涤剂法、氨基酸自动分析仪、原子荧光光谱和原子吸收光谱、乙酰丙酮法和热水提取多糖法分别对菌草香菇和木屑香菇的水分、灰分、蛋白质、纤维素、氨基酸、重金属、甲醛、香菇水溶性多糖等的含量进行比较研究。

To select five blood samples for cross match with mixed field agglutination in order to screen the best disposal methods by CaCl2 centrifugalization method and EDTA-Na2 method which were designed by ourselves, To select another cryoprecipitate which were diluted 1: 10 and 1: 15 after mimesis do cross match and antibody screening with CaCl2 centrifugalization method and EDTA-Na2 method.

用自行设计的CaCl2离心法和EDTA-Na2法对5份交叉配血中有混合外观凝集的血样本分别进行处理，筛选出最佳处理条件；另选取一份冷沉淀，按1：5和1：10稀释后用CaCl2离心法和EDTA-Na2法分别模拟进行交叉配血和抗体筛选。

MethodsThe resin suitable for separation of chromone glycoside was determined by static absorption experiment on seven different kinds of resins, and further more, the absorption and elution of chromone glycoside on the selected resin were studied by fixed bed absorption experiment. ResultsThe AB-8 resin was founded to be suitable for separation and purification of chromone glycoside.

方法采用静态吸附实验考察D101，AB8，S8，X5等7种大孔吸附树脂对升麻苷和5O甲基维斯阿米醇苷两种色原酮苷的吸附效果，筛选出吸附性能较好的树脂；对所筛选出的树脂，进一步采用固定床吸附法分离玉屏风提取物中色原酮苷，探讨主要参数对色原酮苷固定床穿透曲线及洗脱曲线的影响。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。