筛选

Hygromycin is one of the most widely used antibiotics in plant genetic engineering, and in this study we examined the hygromycin-resistant concentrations on tobacco seed germination and leaf explants regeneration.

潮霉素是植物基因工程研究中使用较为广泛的转化体筛选抗生素，本文对烟草种子萌发及叶片外植体组织再生进行潮霉素抗性浓度筛选与研究。

The results showed that(1) the fungi can produce high quantity laccase up to 1 920 U/L on 15 days;(2) its metabolites including terpenol and sesquiterpene alcohol were all natural VOCs of tobacco;(3)the zymotic fluid applied on cured tobacco can apparently reduce the lignin of cured tobacco by 8% and the smoking quality .

采用木质素含量较高的烟梗为筛选材料，分离筛选到一株具有降烟草木质素功能的真菌，并测定了该菌的产酶活性、代谢物中挥发性成分及粗酶液在烟草快速陈化中的作用。

Then the recombinant plasmid transformed E.coli.JM109. One positive clone was obtained from the libraries with casein flat and ELISA sieve method.

用酪蛋白平板法和酶联免疫吸附的活性筛选方法从基因文库中筛选到一株产海洋低温碱性酶的阳性克隆（命名为pHH1）。

In the present experiment, the screening techniques for salt tolerance and mutants of strawberry leaf and anther callus in vitro were studied, and primary screening system for salt tolerance evaluation was established. It was studied the sensitivity to mutagen of different strawberry cultivars and different tissues in vitro.

本试验以草莓离体叶片及花药愈伤组织为试材进行了耐盐突变体的诱变及筛选研究，初步建立了草莓耐盐突变体诱变筛选的技术体系，研究了草莓品种以及离体组织对诱变剂的敏感性，并且对不同诱变处理再生植株的耐盐性差异进行了比较。

Dr. Tilson and colleagues developed a screening checklist tool based on the American Academy of Pediatrics and American Diabetes Association guidelines with some modifications. Participating physicians were to screen all children aged 10 to 18 years for elevated BMI. Children with a BMI higher than the 85th percentile were assessed for the risk factors of race/ethnicity, family history of type 2 diabetes, acanthosis nigrans, and hypertension (defined as systolic blood pressure higher than the 90th percentile for age and height).

Tilson医师与同事根据美国小儿医学会与美国糖尿病医学会的治疗指引进行部份修改，研发了一种核对清单；参与的执业医师筛选所有BMI过高的10至18岁儿童，BMI高于前15%的儿童接受筛选种族／信仰、第二型糖尿病家族史、棘状黑色素瘤与高血压（以高于同年龄与身高前10%的收缩压来定义）。

For obtaining the aneuploid in rice,the primary trisomic from triplo-1 to triplo-12 and the aneuploid derived from 1992~1995.From the offspringe of triplo-10, a primary trisomic of chromosome 10 of this variety,an isotetrasomic triplo-10-1 was obtained.

在水稻上，为了获得这类遗传材料，从中籼3037同源三倍体后代产生的非整倍体中，在筛选初级三体的同时，进行了等臂染色体变异材料的筛选。

Objective To screen the protective antigen of G.intestinalis and set the foundation for immune reseach of vaccine. Methods The trophozoite antigens were obtained with in vitro culture system of Giardia lamblia Stile. After further separation, purification and activity verification, the antigens were used to immunize the BALB/C mice divided into different groups,then indexes associated with immunity were determined. Through challenge infection, the most protective antigenic component of G.intestinalis were selected based on its anti-infection ability.

目的 筛选蓝氏贾第鞭毛虫的保护性抗原，免疫疫苗的研制奠定基础方法利用已建立的蓝氏贾第鞭毛虫滋养体体外培养系统，获取滋养体抗原，并进步分离、纯化、活性鉴定；然后分组免疫小鼠，检测相关免疫指标；再行攻击感染试验，视其抗感染能力，筛选出保护性强的抗原组分。

The main results were showed as follows:Through screen and identification of morphology in M_2 and M_3, respectively, the mutants with mutative traits of plant were found. In which, 11 mutations were leaf variations, including light-green leaf, deep-green leaf, durative yellowish leaf, temporary yellowish leaf, inlaid yellowish leaf, wrinkly leaf, smooth-edged curly leaf, splitting-shaped curly leaf, peduncular-shaped leaf, round leaf and larger leaf, 12 were mutations on plant type, including excessive branches, lacking branches, highness, dwarf-like, erect posture, reclinate posture, strong stalk, slender stalk, tufty branches, wrinkled plant, purple stalk and hair-covered plant, 14 were flower variations, including dense flowers, light-yellow petals, white petals, white-mosaic petals, wrinkled petales, back-rolled petals, narrow petals, larger petals, smaller petals, apetalous flowers, variable-numbered petals, fertile pistil protraction, sterile pistil protraction and multi-anther, and 5 were physiologic mutants, including genic male sterile, cytoplasmic male sterile, bud-dead, and early and later bloom.

主要结果如下：通过田间M_2筛选和M_3重复鉴定，在田间农艺性状方面共获得浅绿叶色、深绿叶色、转绿黄化叶、持续黄化叶、边缘黄化叶、皱缩叶、光叶型卷叶、裂叶型卷叶、薹叶形叶、宽圆叶和大叶等11种不同的叶部性状突变体；同时还筛选到多分枝、少分枝、高杆、矮杆、株型紧凑、株型松散、粗茎、细茎、丛生分枝、整株皱缩、紫茎、整株被毛等12种植株性状突变体，密花、浅色花瓣、白色花瓣、白斑花瓣、皱瓣、卷瓣、窄瓣、大瓣、小瓣、无瓣、花瓣数目不定、可育型柱头外露、不育型柱头外露和多雄蕊等14种花器性状突变体，以及细胞核雄性不育、细胞质雄性不育、死蕾、早花和迟花等5种生理性状突变体。

The results showed that the tested results by PCR and HPLC were identical, the 22 fine Oo strains screened from grape production area produces no histamine, tyramine, nor putrescine, therefore, from the point of view of biogenic amines, 22 fine Oo strains screened in China possesses fairly high safety in metabolically biogenic amines.

结果表明，PCR检测结果与HPLC测定结果一致，从我国葡萄产区筛选的优良酒酒球菌均不产生组胺、酪胺和腐胺，从生物胺角度，我国筛选的22株优良酒酒球菌具有较高的生物胺代谢安全性。

We also found that the Ubi promoter from Zea mays was active in cotton embryogenic callus.

轰击后直接用100 mg/L的卡那霉素进行筛选优于梯度筛选。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。