筛选

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

Firstly, This paper processes geometry filtration based on character of orbit's apogee and perigee after orbit model is analyzed and based. Secondly Space Debris is separated into two kinds, coplanar and not coplanar according as location interrelation of target and debris. In Dynamic filtration's processing, considering prediction time limit and orbit perturbation, secular effects which have increaser effect is considered so that some need catching danger time point is appeased, some law about time when target and debris pass intersectant line each other is inferred, when debris and object are in different plane and some similar law also is inferred otherwise. The time list is arithmetical progression. These laws can improve calculation efficiency remarkably; Lastly orbit predict is a course approaching least distance. Danger time point in the last step become beginning time point from which debris and object's position and velocity are calculated by SXP4 Model which is an analytic model.By result, determine that relative movement trend is closer or apart, farther calculate the interval apart from time when predictive least relative distance occurs. Least relative distance and time when least distance occurs is calculated finally. It is a fast approach course adopting varied interval.

本文在分析、建立轨道摄动计算模型的基础上，首先采用基于轨道远、近地点特征对大量编目在册的空间碎片进行初步几何静态筛选，然后根据碎片与目标轨道面的相对位置特点把碎片分为异面与准共面两种情况以进行动态筛选，在筛选过程中考虑到预报时限长度和轨道摄动影响，引入了轨道摄动中具有积累效应的长期项以满足危险时间点的捕捉要求，利用了长期项摄动变化线性的特点导出了碎片与目标异面情况下各自过交线的时间序列成准等差数列的规律性和准共面情况下类似的规律性，从而显著地提高了前期轨道筛选的计算效率；最后对剩余危险碎片和目标飞行器利用SXP4轨道预报模型在前期轨道筛选的基础上以各个危险探测时间点为预报的时间起点，并根据预报计算结果，分析目标和碎片在该时间点的运动趋势是相互远离还是相互接近，推出预计最小相对距离发生时刻相对此刻的时间长度，得到其随时间变化的局部最小相对距离以及危险距离发生的时刻和相对速度，这是一个采用变时间步长、快速逼近最小相对距离的过程。

Objective To establish a scientific and perfect system of screening index to provide scientific information and Decision basis for the establishment of Appropriate Health Technique source and the admittance of AHT.

目的建立一套科学、完善的农村卫生适宜技术筛选指标体系，为国家适宜技术库的建立和适宜技术的准入提供科学信息和决策依据；建立农村卫生适宜技术筛选模式，为基层适宜技术的筛选提供一套有效、可行的筛选方法。

YTS series circinal cylinder is a rough choosing equipment in the waste paper recycling product line .Fixing it behind waterpower pulp machine to process the big and heavy impurities which come from the waste paper pulp coming out the waterpower pulpmachine.To reduce the in order to ensure the quality.

YTS系列圆筒筛是废纸回收生产线中的一种粗筛选设备，设置于水力碎浆机之后，对于从水力碎浆机中出来的废纸浆进行筛选，除去大、重杂物，减轻后面流程中筛选设备的负担，以保证筛选质量。

After improvement method of sieving. It can proceed the sieving to the single species pesticide liquid, and also fit to multi-specieses pesticide liquid or unknown pesticide liquid, the ratio of volume does not completely together whole 10. Compare with the former, the later has some advantages like easy to operate, economic, easy to control in operating temperature (30℃), breadth application and sieve speed quick (10 min/each), etc. It is very suitable for pesticide factory to produce emulsifiable solution.

改进后的筛选方法，二者的体积比则不凑整10，既可对单品种农药液进行筛选，也可对多品种农药液或未知农药液进行筛选，与前者相比，后者具有操作方法简便、经济、操作温度易控制(30℃)、应用范围宽、筛选速度快（10min/个）等优点，非常适合农药厂家在生产乳油时应用。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

The antifatigue function of more than forty kinds of insect,fungi,bacteria and algae was studied and sifted first in this study by detecting swimming endurance and biochemical indexes. Then,in order to choose the better ones from the compoud additives formulas composed of L-carnitine, taurine, octacosanol,vitaminE and insectA ,a cross design experiment was done. InsectA was discovered in the first experiment that it greatly improved the antifatigue function in mice. Finally, the acute toxicities, thirty-day feeding effects and the mechanisms of No. 1 antifatigue compoud additives(No.1ACF) were studied.

本研究首先通过小鼠游泳耐力试验和血液生化指标的测定对40余种昆虫、菌藻的抗疲劳作用进行了筛选；在此基础上，应用正交设计方法对以L-肉碱、二十八醇、牛磺酸和本实验筛选出的抗疲劳功效较好的昆虫A为原料的配方进行了抗疲劳作用筛选，并对筛选出的最佳配方，即抗疲劳复方添加剂1号配方进行了小鼠急性经口毒性试验、30天喂养试验和抗疲劳作用机理研究。

In this study 2mG2-epsps gene was used to be a selection marker gene, and a series of experiments were designed for determining the time of their screening and selection pressure. The results showed that 1 mM glyphosate isopropylamine and 15 days screening can be effectively inhibited non-transgenic maize callus tissue differentiation. So this research work has provided a safe and effective screening system for corn and other monocotyledons crops.

实验中以2mG2-epsps基因作为筛选标记基因，为确定其筛选时间及筛选压力，进行了玉米愈伤组织对不同浓度草甘膦的抗性实验，确定向培养基中添加浓度为1mM的草甘膦异丙胺盐筛选15天能有效的抑制非转基因玉米愈伤组织的分化，本研究的再生植株的阳性率为45%，为玉米及其它单子叶作物转化提供了一种有效、安全的筛选体系。

If you create a local filter in a form or subform that already contained a filter from a previous working session , the new filter replaces the previous one.

如果在已包含以前会话中筛选的窗体或子窗体中创建新的本地筛选，新筛选将替换以前的筛选。

What this invention has function of take a breath one kind publicly is wall hung inhaler, include: Noumenon, air suction opening, filter component, send fan, outside air blowhole, bypass, fender and eduction mouth; Outside air blowhole is formed go up in noumenon; Bypass is formed at the back of what the component filters inside noumenon, form outside air to flow to the passageway that sends fan directly; Fender setting is filtering on the face of have a common boundary of component and bypass, its position prep above filters component; Eduction degree of lip-rounding is become in noumenal before the bottom of face plate.

本发现亮相了一类具备换气效能的璧挂式空气air筛选器，包含：本体、吸进口、筛选零件、送扇子、外面空气air通气眼、旁路、档扳和排岀口；外面空气air通气眼产生在本体上；旁路产生在本身体之内筛选零件的后头，型成外面空气air直接流向送扇子的路线；档扳设定在筛选零件与旁路的边界面上，其位置高于筛选零件；排岀口产生在本体的前头扳的下部。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。