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Genetic diversity among 7 populations of Trillium tschonoskii were analyzed by using inter SSR and amplified fragment length polymorphism molecular markers. The results were as follows:(1)ISSR: 12 out of the 100 primers showed consistent banding patterns and amplification, a total of 135 bands were produced with an arrange of 250-2500bp, 46 of which (34.07%) were polymorphic.

应用锚定SSR和限制性片段多态对7个延龄草居群的遗传多样性进行了分析,结果如下:从100个引物中筛选出12个ISSR引物,在250-2500bp之间共扩增出的135个条带,其中多态性带为46,占34.07%。

Sherlund's nephew Shane manipulated the long arm of a Caterpillar excavator to feed the dirt from the pile into a trommel: a big rotating barrel with water flowing through it that spits out large rocks on one end and directs silt and small stones into a sluice, a long bed of metal louvers that trap the gold.

旁边是舍伦德的侄子谢恩,他熟练地操纵着卡特彼勒挖掘机的长臂,将成堆的泥土倒进矿石筛:这是一个旋转式的桶状流水分离装置,一端喷出大石头,一端将泥沙和小石子冲进由金属条板制成的长形斜水槽,用以提取出黄金。

According to the situation of our country, an effective way of clean coal production is to increase the ratio of coal washing, which inevitably will generate a large amount of residues including coal washery sludge and gangue causing secondary pollution to the environment.

根据我国国情,加大煤炭洗选比例是洁净煤生产的一条有效途径,这势必产生大量的洗煤泥、洗矸石等筛余物。

The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变;沉淀回收的PCR产物以20μl变性上样液(95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝)溶解后热变性为两条DNA单链后,上样于8%非变性聚丙烯酰胺凝胶(含5%甘油),700伏电压、4℃电泳16小时。

The most common architectural pattern was cribriform (73%), followed by fused/poorly defined glands (55%), cords/single cells (11%), and solid sheets (5%). Nuclear enlargement was observed in 45 of the 55 studied cases (82%). Prominent nucleoli were either absent or infrequent in 38 cases (69%).

癌细胞最常见的组织结构是筛状(73%),接下来是融合的/不成形的腺管(55%)、条索状/单个细胞状(11%)、实性巢团状(5%)。55例中有45例(82%)可见细胞核增大。38例(69%)见不到或罕见显著的核仁。

Fingerprints of broiler chickens divergently selected for very low den sity lipoprotein in fat line and lean line of the third generation were studied using inter simple sequence repeated technique. Geneti c polymorphism and genetic similarity coefficient between two groups were analyze d. Ten primers were screened from 60 SSR primers and 62 sites were deprived. Of which 39 bands were polymorphic sites and the frequency was 63 percent.

通过对初步进行血浆极低密度脂蛋白双向选择的三世代高、低脂群肉鸡进行ISSR指纹分析,评定其遗传多态性及两群体间的遗传相似系数。60个引物筛选出10个引物用于检测分析;共扩增出62条谱带,其中39个是多态性片段,多态率为63%。

ISSR analysis of 11 main cultivated and spread varieties in Juglans Silillata Dode in Yunnan was conducted with new-born leaf as material, based on an optimal ISSR reaction system established and 8 primers screened.

以核桃当年萌发的新叶为材料,在建立了良好的反应体系基础上,筛选出8条引物对云南省11个主要栽培和推广的核桃品种进行了ISSR分子标记研究。

After the second PCR amplification, sixteen differential bands were screened out, of which eleven were co-expressed in P35 female and P45 male, five were only expressed in female at P45, which might underlie the genetic mechanism of the RA dimorphic development in white-rumped munia.

二次PCR扩增后,进一步筛选出16个差异条带,其中11个为P35雌性和P45雄性共同表达的,5个是P45雌性特异表达的。

If they have had any shred of fear for the rankless uniform on Sanots, that glance has dissolved it all, because he is trembling wildly from head to foot.

如果他们对许三多那身没衔没章的军装还有一星半点的忌惮,这一看也全泡了汤,因为许三多两条裤腿都玩命地筛着糠。

The DNA was extracted from mature Spinacia oleracea L.separately by the method of CTAB, then used as template after mixed by gender to be amplified with the optimized RAPD reaction condition. A total of 160 primers (10 bp) and 40 pairs of primers were screened, 44 primers of which produced clear patterns.

菠菜为雌雄异株植物,用CTAB法提取其雌雄成株幼嫩叶片总DNA分别构建雌、雄株的DNA池,以之为模板,用已优化的RAPD体系扩增,在160条随机引物(10 bp)和40个引物对中筛选出44个扩增效果较好的单引物,再从中进行进一步筛选。

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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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