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Objective To evaluate the screening value of the dipstick dye immunoassay and enzyme-linked immunosorbent assay for schistosomiasis diagnosis in the field.

目的评价胶体染料试纸条法和酶联免疫吸附试验两种试剂盒在血吸虫病现场防治中的筛查价值。

Objective To evaluate the eff ec t and cost of Dipstick Dye Immunoassay for mass screening schistosomiasis in the field.

目的评价胶体染料试纸条试验血吸虫病大规模现场筛查中的效果与费用。

on the basis of preparatory screening test, the bacteriostasis of 5% biofungicide were compared with other 5 fruit-vegetable fungicides to 5 kinds of diseases of causing from Botrytis squamosa etc.

在初筛的基础上,比较5%仿生杀菌剂与其他4种果蔬常用保鲜剂对葱磷葡萄孢霉等引发5种果蔬贮藏期病害的抑菌效果,得到了25条毒力曲线及相应的EC50值。

This design divide sieve plate into three plates of different tilt angle to level surface, supplying with a shortcut to resolve the problem mentioned above.

本设计把筛面分成不同倾角的三段,为解决这个问题开辟了一条简捷的路子。

In order to solve these problems, a new type of sifters——the molten sifter has been developed and after the use of a certain period of time has reached its anticipative aim and the maintenance fees are greatly been red.

针对上述问题,开发出了铸石篦条筛,经过一定时间的使用,达到了预期的效果,并且大大降低了维修费用。

Fourteen wampee cultivars collected from Guangdong and Guangxi were used for RAPD analysis in this study. 16 primers selected from 150 random primers were used to amplify 87 DNA fragments and 57 bands (54.0%) showed polymorphisms.

摘 要:对14个黄皮品种进行 RAPD 分析,从150个随机引物中筛选出16个能在种质间表现出多态性的引物,共扩增出 87 条谱带,其中多态性带47条,多态性比例为54.0%。

Four primers screened from 17 primers were used to analyze genetic diversity between one population in the mine deposits and one in noncontaminated soil, the results showed that a higher level of DNA diversity was detected in the plants grown in the mine deposit. The results above indicated that Commelina communis grown in copper mine deposits had evolved adaptation under heavy metal stress, and such adaptation had a geneic basis.

从17条引物中筛选出4条对其中1个铜矿山种群和1个正常土壤种群进行种群内的遗传多样性研究,结果显示污染种群内基因遗传多样性水平高于未污染种群,上述结果说明了在铜胁迫下矿山鸭跖草种群已经进化了对铜污染的适应性,并且较高水平的遗传多样性可能是植物适应重金属污染胁迫环境的基础。

Thirty-seven accessions of wild Dactylis glomerata L. were used in assessment of genetic diversity by means of amplified fragment length polymorphism. Nine primer pairs were selected from 64 preparations and then used to analyze the plant materials.

应用扩增片段长度多态性技术,从64个引物对中筛选出9个,分别对37份鸭茅种质进行扩增,共得到400条清晰条带。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板,挑取其中的白斑经活化培养后,运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板),即5,000 余个重组子克隆的高质量质粒DNA;提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction,聚合酶链式反应)扩增出重组子插入片段,标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5,000 余条5′端EST 序列的测定工作,初步筛选出4,747 条质量较好的EST序列,经筛选得到的EST中90%以上具有大于500bp 的可读序列,每一EST序列中不可读碱基数小于5%。

In cluster group analysis, the four kinds of color-flowered were divided into 2 styles; at the first, the red color and red-white color were grouped together, then they grouped with purple color into one cluster and the white color was single.

共筛选出12个特异引物,扩增出84条带,具有特异性的谱带为28条,占总带数的33%,在聚类图分析中,把4种花色一串红划分为两大类:首先是红色和红白双色聚为一类,然后和紫色聚在一起,白色单独为一类。

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