筛条

Results: 63 genes were up-regulated and 64 genes were down-regulated in the 127 differential expression genes.

结果：共筛选出127条符合标准的差异表达基因，63条呈上调表达，64条呈下调表达。

The RAPD system of Gladiolus was optimized. Of the 12 random primers analyzed in this research, 9 primers can show steady and polymorphic amplification profiles.

建立了适用于唐菖蒲RAPD分子标记的反应体系，从12条随机引物中筛选出9条可扩增出多态性条带的引物。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。

In screens 20 from 100 10bp along with the machine-drawn to increase the effect to direct the thing well to carry on increases, altogether has 188 bandings,118 are the polymorphism belt, accounts for 62.8%, average each DNA polymorphism belt number which directs the thing to increase is 5.90, has promulgated the sweet potato variety heredity multiplicity; construct the shape chart called UPGMA to reflection variety diverse , will supply to try 31 variety divisions will be 4 broad headings, has promulgated between Hunan sweet potato variety from the molecular level and with the foreign country variety between sibship.

从100个10bp的随机引物中筛选出20个扩增效果较好的引物进行扩增，共产生188条带，其中118条为多态性带，占 62.8%，平均每个引物扩增出的DNA多态性带数为 5.90条，揭示了甘薯品种的遗传多样性；根据扩增结果构建出反映品种多样的UPGMA聚状图，将供试31个品种划分为4大类，从分子水平上揭示了湖南甘薯品种之间及其与外域品种之间的亲缘关系。

Results Apart from100%of delineating of the four rectus muscles,optic nerve and lacrinal gland,CT was able to depict not only about50%of oblique muscle,superior ophthalmic vein,ophthalmic artery and the trochlea,but also musculotendinous ring,aterior ethˉmoidal artery,posterior ethmoidal artery,medial and lateral long posterior ciliar artery,central retinal artery,inferior ophˉthalmic vein,supraorbital nerve,lacrimal sac,nasolacimal duct etc.

结果 眼眶水平CT断层扫描除对4条直肌、视神经、泪腺100%显示外，上斜肌、眼上静脉、眼动脉、滑车的显示率在近半数。另外，肌腱环、筛前筛后动脉、内外侧睫状后长动脉、视网膜中央动脉、眼下静脉、肌支动脉、眶上神经、泪囊、鼻泪管等也可显示。

Based on the higher alcohols and lactic acid metabolism theory, a method was developed to low higher alcohols capability Saccharomyces cerevisiae strains by screening high lactic acid dehydrogenization enzyme activity in this study.

本研究对Y1110菌株及其出发菌株、突变等株16株不同来源的酵母菌株进行全基因组RAPD分析，建立并优化了酵母RAPD扩增体系和分型系统，从20条随机引物中筛选出了3条具有较好的扩增多态性，共扩增出34条RAPD谱带，多态性为85.3％；获得了稳定清晰的RAPD指纹图谱。

The average percentage of polymorphic bands are 72.06% for ISSR and 66.67% for RAPD respectively. The genetic diversity of ginkgo revealed by ISSR (the average effective number of alleles is 1.8780, the average Nei"s gene diversity is 0.4652, and the average Shannon"s information index is 0.6574) are all higher than those by RAPD (the average effective number of alleles is 1.7842, the average Nei"s gene diversity is 0.4317, and the average Shannon"s information index is 0.6211), while the standard errors of the parameters estimated by ISSR were lower. Therefore, ISSR molecular markers are more suited than RAPD molecular markers when testing the genetic diversity of ginkgo populations and determining the genetic relationship of among populations or among individuals which are much similar hereditarily.

本文应用RAPD和ISSR两种分子标记技术，选取中国5个可能的野生银杏居群共计75个样品，对其遗传多样性进行了研究，得出以下结论：(1)用筛选出的12个RAPD引物和10个ISSR引物进行PCR扩增，分别扩增出65条和68条重复性高、清晰的条带，多态性位点百分率分别66.67%和72.06%，ISSR揭示的银杏遗传多样性(平均有效等位基因数目为1.8780，平均基因多样度为0.4652，平均信息指数为0.6574)高于RAPD所得到的结果(平均有效等位基因数目为1.7842，平均基因多样度为0.4317，平均信息指数为0.6211)，其所估算参数的标准差要低于RAPD所估算出的值，因此，在研究亲缘关系非常近的银杏物种的遗传多样性并试图确定居群间或个体间的遗传关系时，ISSR分子标记技术比RAPD分子标记技术更为合适。

Five polymorphic primers with good, clear bands were got, screening fromseventy-five ISSR primers. These five pairs of primers were used to amplificaterespectively the genomic DNA of 66 different Pterocarya stenoptera individuals and atotal of 45 bands were amplified with 43 polymorphic bands and percentage ofpolymorphic bands 95.6%.

2从75条ISSR引物中筛选出5条多态性好、谱带清晰的引物，用这5条引物对66个枫杨叶样的基因组DNA进行了PCR扩增，共得到45条带，其中多态性带43条，多态性百分率为95.6％。

Rod of sieve sieve drum strength, high fatigue, sieve Aperture uniform accuracy level of similar foreign products, Aperture uniform, 90% of the gap between the deviation of ± 0.025mm.

棒条筛筛鼓强度大，抗疲劳强度高，筛缝宽均匀，精度达国外同类产品水平，缝宽均匀，90％缝隙偏差在±0.025mm之间。

Main Products---The complete equipment for recycling waste tire: Tire cutter, Strip cutter, Lump cutter, Bead wire separator, Shredder for rubber, Large and Small belt conveyor, Large and Small vibrating sieve, Magnetic separation equipment.

主要产品--废旧轮胎处理成套设备：切圈机，切条机，切块机，口圈分离机，破胶机，大输送机，大振动筛，小输送机，小振动筛，磁选设备。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。