筛出

Specific bands of D genomes and AB genome in four diploids, four tetraploids and four hexaploids, respectively, could be also amplified by means of M70/E5 1 and M711E5 1 primer combinations screened from twenty pairs of AFLP primer combination for test.

对二倍体、四倍体和六倍体小麦各4个材料的DNA进行AFLP扩增，从20对引物组合中筛选出M70/E51和M71/E51两个引物组合能扩增出D染色体组或AB染色体组的特异性带。

Four strains which could effectively degrade dye were separated from bark with brown or ivory white locus ferrugineus.One strain with the strongest MnP activity was selected by measuring enzyme activity with natrium lacticum,which is

本研究从长有褐色或者乳白色锈斑的树皮上分离出4株可以降解染料的菌株，结合乳酸钠法测酶活，筛选出1株产酶能力较高的菌株W3，其MnP活力为8.59Wg，经形态鉴定为黄孢原毛平革菌。

In this experiment, some strains of yeast were separated from koumiss in 4 regions of 3 nations, 3 strains of which were chosen to make the koumiss by the mixed fermentation under the experimental condition with the lactic acid bacteria. In fermentative process the alcohol content variation was measured.

从3个国家4个地区的酸马奶中共分离筛选出多株酵母菌，并筛选出3株酵母菌在实验室条件下与乳酸菌进行混合发酵制成酸马奶，测定发酵过程中的乙醇含量变化。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

According to the physiochemical and microbacterial results of them, two strains No. RC212and DV10which presented powerful fermenting performance and good production conformability were selected as test strains for "Industrialized Production Techniques Research of Rosa Roxburghii Tratt Claret".

经比较筛选出RC212号葡萄汁酵母与DV10号汉氏德巴利氏酵母两菌株，其均表现出较强的发酵性能及生产适应性能，为&刺梨干红工业化生产技术研究&提供了试验菌株。

If there is no pale cloud or mist, the sky would have been a dull without colorful sunglows.

如果没有轻云薄雾把日光筛漏出五色霞彩来，天空该多么单调枯燥！

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Recent years, by absorbing advanced and latest experience of abroad and home, we optimize our design, adopt top leading technology, and manufacture top quality class stone crusher and grinding equipment such as PE Jaw crusher series and YGM Grinding mill series, and PCL VSI crusher series.

近年来我公司在吸取过内外先进经验的基础上，优化设计，采用国内外技术和工艺，制造出了以硬岩反击式破碎机、颚式破碎机为核心的破碎筛分成套设备，成为国内外建筑行业以及高等铁路、公路的首选设备。

The diabetes II patient which through sieves to the Liaoning Province Shenyang Shen River District wind and rain world community inhabitant's community looks up, discovers the diabetes patient newly to send case of illness, when complication does not have any illness complication, causes it separately to accept the self-treatment, the community tracks the treatment, and to all groupings patient in half year, a year, two years, three years carries on the track to pay a return visit.

通过对辽宁省沈阳市沈河区风雨坛社区居民的社区筛查，找出糖尿病患者新发病例，并发病时无任何并发症的糖尿病Ⅱ型患者，使其分别接受自我治疗，社区跟踪治疗，并对所有分组患者在半年，一年，两年，三年进行跟踪回访。

Our main products: dredging ship, pumping sand barge, sand screening machine, Dump Sand Barge, gravel Featured equipment, gold machinery, iron ore extraction equipment, river pebbles and weathered broken equipment, screw sand washing machines, conveyor-type automatic loading sand machinery, etc.; a total of ten series more than 30 specifications for the user to choose, and geographic environment based on user design and manufacture of special products, factory production of mechanical energy at the same time the completion of dredging excavation, screening, separation of several procedures, one-time production of qualified products, divided into sand, stone and waste in the advantage of high efficiency and low cost.

本厂主要产品：挖沙船，抽沙船，筛沙机，自卸运沙船，砂石精选设备，淘金机械，铁砂提取设备，河卵石及风化破碎设备，螺旋洗沙机，输送式自动装沙机械等；共有十大系列三十多个规格供用户选择，并根据用户地理环境设计制造异型产品，本厂生产的挖沙机械能同时完成挖掘，筛选，分离几个程序，一次性生产出合格产品，分为沙石，中石及废料，其优点效益高，成本低。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。