等量

The transesterification reaction were studied under organic solvents systemThe dosage of diatomite immobilized lipase of Enterobacter aerogenes is 1000 U, ethanol as acyl receptors(alcohol:oil molar ratio is 4:1,2 times add),5 mL n-hexane, 180 r/min,35℃,48 h,and the conversion rate of 92.91%.

有机溶剂体下催化生产生物柴油硅藻土固定化产气肠杆菌脂肪酶用量为1000 U，乙醇为酰基受体(醇：油摩尔比为4：1，2次等量加入)，5 mL正己烷，180 r/min，35℃，反应48 h，转化率达92.91%。

Leukocyte proteins concentrations were determined with Bradford Method. Phosphoproteins in the collected leukocyte proteins were enriched by BDTM Phosphoprotein Enrichment Kit. Finally the enriched phosphoproteins were separated by SDS-PAGE and dyed by argentation, and the electrophoresis map of SDS-PAGE was analyzed by Scion Image Tool.

刺激组加入表皮生长因子(40 μg/L)；抑制剂组加入细胞外信号调节激酶特异性抑止剂UO126 （10 μg/L）和表皮生长因子（ 40 μg/L）；对照组加入等量的培养基，用Bradford法测定细胞蛋白质含量，BDTM Phophoprotein Enrichment Kit分离富集白细胞磷酸化蛋白质，SDS-PAGE分离磷酸化蛋白质，银染法染色，采用Scion Image软件分析电泳结果。

Methods autohemic blood from femoral artery of dogs was taken and injected to one side pleural cavity with the same volume of normal saline and air to establish a model of hemopneurothorax on dogs .then 24 the dogs were divided into experimental group and control group at random with 12 dogs in each group. to observe and analyze the time of chest tube insertion,the intensity of resistance to pulling and drainage effect between the thoracentesis technique of invented minute invasion closed-chest drainage and traditional closed-chest drainage.

利用家犬自体股动脉穿刺采血注入一侧胸腔、同时注入等量盐水及空气的方法建立家犬血气胸模型，并将其随机分成实验组（n=12）和对照组（n=12），观察分析自制微创胸腔闭式引流装置与传统胸腔闭式引流装置的置管时间、抗拉强度、引流效果等。

Results Approximately 700 protein spots were revealed in equivalent proteins of the promastigotes and axenic amastigotes separated by 2-DE,among which more than 90% protein spots showed equivalent quantity and distribution,with 6 proteins up-regulated and 3 proteins down-regulated in axenic amastigotes compared with promastigotes.

结果 等量的前鞭毛体与纯培养的无鞭毛体总蛋白经双向电泳分离后均可获近700个蛋白点，其中超过90%的蛋白点的分布和相对强度基本一致。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

Bcl-2 mRNA and protein were constitutively moderate expressed in sham operation group.

电针组与甲基强的松龙组相比TUNEL阳性细胞无显著性差异。2）假手术组有中等量的bcl-2 mRNA及蛋白表达。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

In our study,we found thatimmunoprecipitation with an antibody to PICK1 resulted in coprecipitation of ASIC1aprotein.

在缺血再灌注的大鼠皮质组织上，与假手术组相比，等量PICK1结合的ASIC1a量有所增加。

When used cosmetically it can lighten and tone human hair and when mixed with equal parts of shampoo it has been known to strengthen hair too.

使用时，它可以减轻美观和语调时，人的头发和混合与等量的洗发水它一直被称为加强头发太多。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失