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Methods Fifty pairs of fresh colorectal cancer and homologous normal tissues were genotyped with Identifiler Kit and the mutations generated in cancer tissues were determined. The mutation rates, the numbers of locus matched without identical allele (A0), 1 identical allele (A1), or 2 identical alleles (A2) and the number of total identical alleles IA(subscript n were calculated. Frequency distributions of A0, A1, A2 and IA were compared among CR-N group, unrelated individual pairs and full sibling pairs. Discrimination functions were established for individual identification from tumor tissues with discriminatory analysis.

用Identifiler系统对50对新鲜结直肠癌组织及其身源正常组织组进行STR分型，计算CR-N组中变异STR基因座及全不同基因座数(A0)、半相同基因座数(A1)、全相同基因座数(A2)和共有等位基因数IA(下标 n，比较CR-N组、无关个体对组和全同胞对组中上述参数的分布差异，通过判别分析建立判别函数。

Based on the drilling operation of 4 Wells, this article describes the basic characteristic of coalbed methane and technical difficulties in drilling. Some technical keys as borehole quality, fresh Water drilling,fresh water coring, coal-bed methane protection are solved through application of a series of techno1ogical measures such as optimization of rock bit, optimization of drill stem, optimization of drilling parameters and string coring and etc. The experimental performance, especially in fine-grained ...

本文以四口井的施工为例，着重介绍了煤层气井的特点，并指出了钻煤层气井的几个技术难点，通过钻头优选，钻具优选，钻井参数优选，绳索式取心技术等的现场实施和应用，基本解决了井身质量，清水钻井，清水绳索取心，煤层气保护等几个方面的技术难题，文中还介绍了使用中原油田自行设计的中原9508绳索取心工具的取心情况，尤其是在粉煤层地区的取心情况，这将为今后的煤层气开发打下坚实的基础。

A lot of people think doing SEO is to make flow by accident, make a rank, do collect etc, this kind of idea likes a person with a very limited outlook, gutty be in along the body only the satirize in this hill.

很多人误以为做SEO就是做流量，做排名，做收录等，这种想法犹如井底之蛙，有种只缘身在此山中的讽刺。

Nonverbal communication can be further divided into four groups, namely, kinesics, paralanguage, object language, and environmental language.

非言语交流是指言语之外的交流手段，它包括身势动作、副语言、对象语和环境语等四大类。

In the subjective test, it adopted the quantity survey method of psychophysics as the evaluation methods. The evaluation factors included soft feeling, moulding sensation, oppressing sensation, kinesthesia and compositive sensation.

在主观测试中，以心理物理学中的数量估计法为评价方法，评价因子包括柔软感、塑身感、压迫感、运动感以及综合感等。

Click Guangdong net to go up the alliance that buy food " Shenzhen buys food ", there is the market such as body of birds of vegetable, flesh, aquatic product, oily rice, condiment, beverage, maigre, fruit, model, nutrition here, almost the dish in the supermarket, can buy on this net.

点击广东网上买菜联盟"深圳买菜"，记者发现这里有蔬菜、肉禽、水产、油米、调料、饮料、素食、水果、塑身、营养等菜市场，几乎超市里的菜，在这个网上都可以买到。

Perhaps this is your first time Accept the system of medical cosmetic services, we understand your heart at the moment Tantebuan Ogata, the Sunshine beauty of medical experts from institutions operating procedures, surgery summary Surgical risk, and so the analysis to answer, be sure to be assured that you start taking In Taiwan, meet the beautiful

也许您是第一次接受系统医疗美容服务，我们理解您此刻忐忑不安的心绪，阳光医疗美容机构专家会从操作流程、手术概要、手术风险等多方面进行分析解答，务必使您放心走下手术台、迎接美丽变身。

Please use the soft cloth soaked with detergent to wipe off this unit if the stains are found on the bag boy's surfaces of this unit.

如果本产品袋身表面出现污脏，请用沾有洗洁精的软布擦洗，严禁使用强溶剂，如汽油、溶剂等物。

Honey contains glucose 32.9%, fructose 41.4%, acidity 2.13%, enzyme was 17 degrees 9%, 0.27% for sugar content and contains more than 10 types of minerals and proteins, amino acids, vitamins, inorganic acid, such as, not only is the ideal nutrition nourishing food, and can relieve a cough and Moistening Lung, stomachic Tongbian,fang shu heart, and who have completed Yiqi, the effect of longevity.

蜜中含有葡萄糖32.9％、果糖41.4％、酸度为2.13％、酶度为17 9％、含糖量为0.27％，并含有10余种矿物质和蛋白质、氨基酸、维生素、无机酸等，不仅是理想的滋补营养食品，而且可以止咳润肺、健胃通便、防暑清心，并有补身益气、延年益寿之功效。

Tattoo Safety Precautions explained so you know the right questions to ask you local tattooist before you leave for the parlor.

纹身安全措施，你知道正确的解释等问题要问你，你离开之前，为当地文面屋。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。