等浓度

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Surfactants have a big effect on the pigment concentration and yield value, and indirectly affect the viscosity of ink.

表面活性剂等助剂的加入通过对颜料体积浓度和油墨屈服值的影响，间接影响油墨粘度。

A three-dimensional world ocean carbon cycle model with ocean biota pump is developed. In the model, the atmosphere is represented by a well-mixed box of CO 2 , where CO 2 is exchanged with CO 2 from the surface water. The carbon cycle model has been numerically integrated for 1 200 years to a quasi-equilibruium state.

建立了一个带生物泵的三维全球海洋碳循环模式，该模式在海气界面上与一个CO2 充分混合的大气箱进行CO2 交换，模式积分 1 2 0 0年达到准稳定态的海水总CO2 、碱度及溶解氧的浓度、表层新生产力、海气分压差等的分布与实测相接近。

The effects on the radial distribution of coarse particle concentration were studied by the two-fluid model.

粗颗粒浓度径向分布是流速脉动、颗粒脉动、颗粒碰撞和径向力等不同扩散作用综合作用的结果。

A mediator-less two-chambered microbial fuel cell was constructed with sodium acetate as substrate in the anode and carbon felt as cathode. The effects of different electron acceptors in the cathode, external resistances value, concentration of sodium acetate and different inoculation ways on electricity generation by the MFC were investigated.

以乙酸钠为阳极底物，碳毡材料为阴阳电极，构建了无介体双室微生物燃料电池(Microbial fuel cell， MFC)，研究不同阴极受体、外接电阻、乙酸钠浓度和不同接种方式等因素对电池产电性能的影响。

Although effects of nitrogen fertilizer on nitrogen accumulation and nitrate reductase in tobacco leaf had been extensively studied, impacts of nitrogen fertilizer on flue-cured tobacco leaf nitrogen metabolism in dry land and paddy field were not well documented.

试验采用裂区设计，以硝态氮和铵态氮两种氮素形态为主处理、5个供氮水平为副处理，在大田条件下研究了水田与旱地烤烟在团棵、旺长、脚叶采烤、腰叶采烤和顶叶采烤等5个生育期烟叶的总氮浓度、硝酸还原酶和谷酰胺合成酶活性的氮肥效应。

Results The main occupational risk factors that existed in the tobacco plant were noise,dust,high temperature and chemical poisons.Monitoring results of analogy projects showed noise intensities were 74.9-92.4 dB,exceeding rate was 47.5%.The concentrations of dust,carbon dioxide and alcohol were 0.2-1.7,953-2 128,5.4-21.5 mg/m3,respectively,which were below occupational exposure limits.

结果该建设项目存在的职业病危害因素有噪声、粉尘、高温、化学毒物等，类比工程各种职业病危害因素的检测结果为：噪声强度为74.9~92.4 dB，超标率47.5%；烟草尘浓度为0.2~1.7 mg/m3，超标率为0%；作业场所生产性热源的综合温度为30.8~36.5℃，均为高温作业点。

Three-effect concentration tank is the newly developed intelligent products, have digital display of intelligent transportation, automatically controlled temperature , liquid location , steam pressure , vacuity , density of the supplies the other words are density , supplies material by itself, collect the lotion ,etc.

自控型三效浓缩器是新开发的智能型产品，具有智能化运程数字显示，自动控制温度、液位、蒸汽压力、真空度、物料密度即浓度、自动补料、收膏等特点。

Now, research replaces the option of index in the various disease appraisement of curative effect to discuss , if with blood plasma 羟 hydrogen can appraise when ketone density is replacing index A flat kind ease pain medicine Kong Shi dose relieve pain effect , is taking ATT( aim at the antibody effect of tetanus toxin) as replacing index to appraise the immunity inhibition researchs of? single clone antibody 5c 8 of the CD 154 in monkey body to get better effect.

目前，已有研究就多种疾病疗效评价中替代指标的选择进行了探讨，如以血浆羟氢可待酮浓度作为替代指标评价阿片类镇痛药控释剂的止痛效果、以 ATT(针对破伤风毒素的抗体效应)作为替代指标评价猴体内 CD154的单克隆抗体5c 8的免疫抑制作用等研究都取得了较好的效果。

In laser assisted microprocessing, the temperature of the spot irradiated by laser has a tremendous impact on the distribution of impurity concentration, the depth of p-n junction , the resistance of ohm contact and so on. In order to improve the performance of the devices, the temperature of the exposed region should be measured accurately.

在激光微细加工中，激光曝光区的温度对激光诱导扩散生成p-n 结的结深、杂质浓度的分布和激光辅助合金生成欧姆接触的接触电阻等具有决定性影响，必须准确测定。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。