等浓度

The response of gas exchange parameters such as net photosynthesis rate, transpiration rate, water use efficiency of leaf, stomatal conductance and intercellular CO2 concentration of 2-years old Periploca sepium Bunge to different soil water content were studied in Loess Plateau with CIRAS-2 portable photosynthesis system.

在半干旱黄土丘陵区，采用CIRAS-2型光合作用系统，在不同土壤水分条件下，对2年生杠柳的净光合速率、蒸腾速率、叶片水分利用效率、气孔导度和胞间CO2浓度等叶片气体交换参数的日变化进行了研究。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

When cellular Ca~(2+) concentration increased, Ca~(2+)与can combinewith calmodin create compound, the latter arose VSMC shrinking through phosphated, and can accelerate express of many cancer gene, such as c-fos、c-myc、c-myb, consequently bring the multiplication and plumped of VSMC.

当细胞内Ca~(2+)浓度升高后，Ca~(2+)与其细胞内受体钙调素(Calmodin，CaM)结合生成复合物，后者通过一系列磷酸化过程引起平滑肌细胞收缩，并可促进多种癌基因的表达，如c-fos、c-myc、c-myb等，从而引起血管平滑肌肌细胞的增殖和肥厚。

Highly ordered SBA-15 sieves have been successfully synthesized in a low concentration liquid inorganic acid medium via the addition of small amounts of phosphomolybdic acid.

通过在低浓度无机液体酸介质中添加少量磷钼酸，成功地合成了高度有序的SBA-15介孔分子筛。N2吸附-脱附、XRD、TEM和水热稳定性实验等检测结果表明：用这种方法不仅可以大幅度降低合成SBA-15分子筛所需液体酸的用量和缩短晶化时间，同时反应产物SBA-15在100℃的水中处理72h后仍然保持六方有序的介孔结构

TiO2 photocatalyzer supported on glass were prepared with sol-gel method. The photocatalytic degradation of methylene blue was studied including the effect of the factors such as reaction time, the initial concentration and pH value on the photocatalytic degradation.

采用溶胶－凝胶法制备了玻璃负载的TiO2薄膜光催化剂，研究了该催化剂对亚甲基蓝溶液的光催化降解，考察了反应时间、水样的初始浓度、溶液的pH等对光催化降解效果的影响。

By changing the photosensitize concentration of C60and TNF,material s environment temperature,and applied electric field,the material s photoconduction is studied,finding out that the materials photoconduction is direct proportion to temperature,increases by the photosensitize concentration and applied electric field,contrasting the photoconductive of PVK+C60and PVK+TNF.

通过改变聚乙烯咔唑中光敏剂C60和TNF的浓度，材料体系的环境温度，以及外加电场场强的大小等，对材料体系的光电导效应进行了分析，发现材料的光电导具有正的温度系数，随光敏剂含量和外加电场场强的增加而增大，对PVK+C60和PVK+TNF的光电导进行了对比。

The relativity analysis indicated that the net photosynthetic rate of F.cymosum had a positive correlation to the stomatal conductance,photosynthetically active radiation,transpiration rate and water utilization efficiency,and negative to the intercellular CO2 concentration.

相关性分析表明，金荞麦叶片净光合速率与气孔导度、光合有效辐射、蒸腾速率和水分利用效率等呈正相关，与胞间CO2浓度呈负相关。

The effect of preparation method of Al-pillared montmorillonite agent, montmorillonite suspending concentration, Al-pillared agent dosage, pillaring reacting temperature, pillaring reacting time are investigated, and the best craft condition has been ascertained. Then the capability has compared with the nature montmorillonite.and Al-pillared montmorillonite.

研究探讨了铝交联剂制备方法、蒙脱石悬浊液浓度、铝交联剂的用量、交联反应温度、交联反应时间等各因素对其制备的影响规律，确定了铝交联改性的最佳工艺条件，进行了与天然蒙脱石和改性累托石的性能比较。

The effect of preparation method of Al-pillared agent、rectorite suspending concentration、Al-pillared agent dosage、pillaring reacting temperature、pillaring reacting time are investigated;The optimum process condition is determined. The capability has compared with the nature rectorite. It is characterized by X-ray Diffraction and infrared spectrum.

探讨了铝交联剂制备方法、累托石悬浊液浓度、铝交联剂的用量、交联反应温度、交联反应时间等各因素对其制备的影响规律，确定了铝交联改性的最佳工艺条件；进行了与天然累托石的性能比较；运用红外光谱、X-射线衍射对所制铝交联累托石进行了表征。

Ti-PILCs were also obtained with pillaring agent prepared from Ti(n-C4H9O)4 and HC1 by Sol-Gel, using purified Na-montmorillonite in Hubei as the starting clay.

以鄂东钙基膨润土经提纯钠化后所得的精制钠基蒙脱石d_(001=1.28nm为基质粘土，以钛酸正丁酯[Ti(n-C_4H_9O)_4]为钛源，采用溶胶—凝胶法制备了一系列钛交联蒙脱石纳米复合材料；并对Ti／土比、H／Ti摩尔比和盐酸浓度等主要合成条件进行了讨论。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！