等时性

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

A non-singular single integral mathematical model is adopted to calculate the external intermittent and alternative longitudinal magnetic field acquired by single axisymmetrical and cylindrical hollow coil t which is coaxial to electrode and excitated by a revisable pulsed current of square wave t in austenitic stainless steel GTA welding.

针对奥氏体不锈钢外加纵向磁场的气体保护钨极电弧焊接时，与电极同轴的单个轴对称空心圆柱线圈在双向脉冲矩形励磁电流作用下产生的间歇交变纵向磁场，采用无暇点单积分磁场的数学模型，对＊TAW焊接外加间歇交变纵向磁场进行了数值计算，该方法具有模型简单、计算速度快和精度高等优点并讨论了该间隙交变纵向磁场的分布、磁场的均匀性及其纵向分量与径向分量的比率等规律及其对焊接行为的影响，认为横磁分量将削弱GTAW外加纵向磁场的有利作用，影响磁控电弧的焊接质量

Results: The main manifestations of imaging in PCCCL appeared as peripheral nodules or masses (6/7) in the right lung. Tiny nodules with coarse spiculate margin, lack of pleural indentation and vacuole sign. When masses of turmor grew larger, smooth margins were found in them. spinous process and cavity were seldom seen. Central mass of left lung superior lobe (n=1), lesion was lobulated, and with smooth margin, which seem others central carcinoma of the lung.

结果：PCCCL影像学上主要表现为右肺周围性肿块或结节(6/7)，结节边缘可见毛刺征，无胸膜凹陷征、空泡征；肿块较大时，边缘光滑，无棘状突起、空洞等征象，1例左肺上叶中心性分叶状肿块，边缘光滑，酷似其它类型肺癌。

The essence of EDID is to set up a normal behavior fuzzy sub collection A on the basis of watching the normal system transfer of the privilege process, and set up a fuzzy sub collection B with real time transfer array, then detect with the principle of minimum distance in fuzzy discernmethodThe innovation point of this paper is : Put forward the method of EDID, can not only reduce efficiently false positive rate and false negative rate, also make real time intrusion detection to become possibility; have independent and complete character database, according to the classification of monitoring program, design normal behavior and anomaly behavior etc., have raised the strongness of IDS; Use tree type structure to preservation the character database, have saved greatly stock space; in detection invade , carry out frequency prior principle, prior analysis and handling the behavior feature of high frequency in information table, have raised efficiency and the speed of detection, make real time intrusion detection to become possibility; have at the same time realized anomaly intrusion detection and misuse intrusion detection, have remedied deficiency of unitary detection method.

本文的创新点是：通过对特权进程的系统调用及参数序列的研究，提出了基于Euclidean距离的入侵检测方法EDID，不仅能有效降低漏报率和误报率，而且使实时入侵检测成为可能；设计有独立而完整的特征数据库，根据被监控程序的类别，分别设计正常行为、异常行为等，提高了检测系统的强健性和可伸缩性；特征数据库按树型结构存储，大大节省了存储空间；在检测入侵时，实行频度优先原则，优先分析和处理信息表中的高频度行为特征，提高检测的速度和效率，使实时入侵检测成为可能；同时实现了异常入侵检测和误用入侵检测，弥补了单一检测方法的不足。

The higher the concentration of manganese sulphamate in the electroforming electrolyte, and the lower the tem- perature of the electrolyte, the more manganese content is in the layers.

在复杂形状零件进行Ni—Mn合金电铸时，选择较低的电解液锰盐浓度、较高的电解液温度和合适的脉冲电流脉宽等工艺条件，有利于改善电铸层锰含量分布的均匀性。

Advaced systolic pressure, sinus tachycardia and tachypnea are found in attack phase.

发作时多伴收缩压升高、窦性心动过速及呼吸加快等。

A pure and single K+ current of ultrarapid delayed rectifier K+ current or transient outware K+ current is respectively expressed on Xenopus Oocytes. This is a moderm pharmacological model for evaluation class Ⅲ antiarrhythmiv durgs, overcomes many defcts such as lacking human fresh cardiac muscle cells as material and co-expression of many currents on cell membrane. A new compound A1998 from Ocean material is evaluated in this model for class Ⅲ antiarrhythmic functions.

5和Kv4.2cDNA转录为mRNA，然后将其分别注入蟾蜍卵母细胞上：在蟾蜍卵母细胞上分别获得纯净、单一的超速延迟性整流钾电流(Ikur，ultrarapid delayed rectifier K+current)和瞬间外向钾电流(Ito，transient outward K+current)表达，克服以往在筛选评价抗心律失常药物时，人新鲜心肌细胞取材困难、多种电流在细胞膜表面共同表达等缺点，从而建立评价Ⅲ类抗心律失常药物的先进药理模型，并筛选具有Ⅲ类抗心律失常药理作用的新化合物A1998。

Methods Ultrasound, introvenus urogram, retrograde pyelography, CT, magnetic resonance urography and transurethral ureteropeyloscopy were performed, and the accuracy rate was evaluated.

方法在诊断肾盂癌时采用B超、静注尿路造影、逆行性肾盂造影、CT、泌尿系磁共振水成像、输尿管肾盂镜检查等多种方法，比较各种方法对早期肾盂癌诊断的准确率。

Among them, 66.75% patients had dyspareunia and vagina xeransis in the following 3 months, and 33,27% 6 months later, but the incidence rate didn't return to pre-operation level (6.1%).

其中手术后3个月，存在性交疼痛、阴道干涩等性生活质量问题的患者占66.75%，手术后6个月时，降至33.27%，但未恢复到手术前(6.1%)的水平。

The Builder shall furnish a guidance booklet at delivery for recommendable distribution of cargo etc. at loaded and ballasted conditions to ensure proper trim, stability and strength data during the service.

建造方交船时应提供一份指导手册用于建议在载货和压载条件下货物等的分布，以使该船在航行状态下具有正确的平衡，稳性和强度。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。