端细胞的

Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

Large numbers of experiments internal and external had confirmed both direct current and square pulse of distal end cathodal could improve peripheral nerve regeneration, because it could speed up transmigration, creeper, growth and development of cell Schwann, derivate growth of nerve fiber; it also increased blood capillary of epineurium, brood blood vessel, amendment ischemic of impaired nerve, it still could accelerate Wallerian

国内外大量的基础实验及临床研究都证实远端负极电流的直流电场及脉冲电场等均有促进周围神经再生的作用，认为其可加速Schwann细胞的游走、爬行及生长发育，诱导神经纤维的生长；电刺激还能促使神经外膜毛细血管数量增多，扩张血管，改善神经损伤段缺血状态，并加速Wallerian变性及崩解组织的清除，为Schwann细胞发育和髓鞘化过程提供充足的能量与物质，同时为神经轴突的再生提供良好的内环境；在神经断端出现瘢痕或神经瘤时，电刺激可增加神经纤维穿越瘢痕的能力。

Results : The telomere length in the SHEE and SHEEC was significantly shorter than that in the normal esophageal epithelial tissue , but maintained a stable length.

结果：SHEE 细胞和SHEEC 细胞的端粒平均长度比正常食管上皮细胞的明显缩短，但稳定维持在一定长度范围内。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

It has been demonstrated that Pin1 was the first essential prolyl isomerase for cell division in both yeasts and mammalian cells.

中文摘要 Pin1 是构酶中第一个被证明於酵母菌和哺乳动物细胞的细胞分及生长过程当中皆必须之蛋白质，以结构与功能性区分，Pin1 可分为N 端的WW domain 和C 端的PPIase domain。

While the tissue spaces surrounding a few blood vessels wasAl and Fg positive,no Al or Fg positive cells were observed.In antemortem injurygroup,diffuse subarachnoid hemorrhage,cerebral edema,swelling or pyknotic neu-rons could be observed.The axons showed irregular swelling and disconnection at1～3h,marked swelling and disconnection at 6h,and retraction ball at 15h whichwas more remarkable at 24h after injury.The space between myelin sheaths andaxons was increased at 3～6h after injury.Tortuous and wavelike myelin sheathswhich adhered on axons incompletely,or even peeled off could be found from 15hto 24h after injury.Perinuclear lysis of Nissl bodies began at 24h after injury.Thenumber of GFAP positive cells in cerebrum and brain-stem increased significantlyfollowed by decrease,and then increased again,but the time courses of the changesin different areas of brain were not same.Al and Fg positive neural cells,mainlysurrounded blood vessels,with diffuse or peripherally distributed positive matter incytoplasm could be observed at 0.5h after injury.The number of Al or Fg positivecells and the intensity of immunoreaction increased with the time of injury.The areaof SYN positivity in medulla oblongata and pons decreased notably 3～6h afterinjury,then return to normal levels and continued to 24h after injury.

生前损伤组，可见广泛蛛网膜下腔出血，脑组织水肿，神经细胞肿胀，晚期神经元固缩；伤后1～3h见部分神经轴突不规则增粗、断裂，伤后6h断端膨大，伤后15h可见收缩球，至伤后24h更为明显；伤后3～6h可见部分神经髓鞘与轴突之间的间隙增宽，伤后15h髓鞘明显曲折，不完全附着在轴突两侧，甚至剥脱，持续到伤后24h；核周尼氏体减少在伤后24h才开始出现；同一部位的GFAP阳性细胞数目随损伤时间发生改变，先增多（最早在伤后0.5h），达到高峰后减少，其后又有增多趋势，但不同部位的GFAP阳性细胞数目增减的时间过程不尽相同，同时，大脑中的GFAP阳性细胞数目也有改变；伤后0.5h，可在脑干组织中见到Al和Fg阳性神经细胞，主要位于血管周围，阳性物在胞浆中呈弥散性分布，但部分细胞的阳性物仅分布于靠近胞膜的胞浆中而呈环状，随损伤时间延长，阳性细胞数目增多，反应强度增加；伤后3～6h，延髓及桥脑中的SYN阳性物面积减少，其后恢复到正常水平，并持续到伤后24h。

The neurosecretory somata were located onthe proximal surface of the medulla terminalia of the eyestalk.Three types ofneurosecretory cells were distinguished on the basis of size, morphology,distribution,form of outgrowth and ultrastructural feature.

河蟹眼柄神经分泌细胞主要分布于眼柄视神经节端髓基部外侧，依据细胞的大小、形态、超微结构特征及离体生长形态区分出三种类型神经分泌细胞。

The article focuses on the aberrant expression and significances of proto-oncogenes, tumor suppressor genes and genes associated with the proliferation process of gastric cells such as telomerase, transcription factor and cyclooxygenase, etc. These genes, alone or synergically, participate in gastric cancer cell proliferation, resulting in an uncontrolled growth characterized by over-proliferation of decreased apoptosis of gastric cancer cells.

本文着重阐述了与胃癌细胞增殖过程密切相关的端粒酶、核转录因子、环氧化酶等基因以及参与细胞周期调控的原癌基因、抑癌基因的异常表达及临床意义，这些基因或单独作用，或相互协同，参与胃癌细胞的增殖，使肿瘤细胞生长以增殖过多、凋亡过少的失控性生长为特征。

In this thesis, we find that dlg is indispensible in the establishment of anterior-posterior and dorsal-ventral polarity of drosophila oocyte. Removal of Dlg function from the posterior follicle cells using the FLP/FRT system leads to disruption of oocyte skeleton reconstruction that is elicited by the failure of those posterior cells to differentiate normally in mid-oogenesis. We demonstrate that abnormity of Notch, JAK-STAT and EGFR signal pathway in dlg mutants contributes to this aberrant differentiation. dlg null mutant also blocks the normal differentiation of two groups of anterior follicle cell-stretched cell and centripetal cell, but not border cell, with a lower penetrance. However unlike the result in posterior follicle cells, Notch and JAK-STAT signaling are both undisrupted in all mutant anterior follicle cells, implying other fate determinants may be involved.

我们的研究发现，后端滤泡细胞中的Dlg在果蝇卵子发生中期卵母细胞前后轴和背腹轴建立过程中也是必须的，PFC中dlg完全缺失型突变引起PFC的分化异常，导致卵子发生中期卵母细胞骨架重组异常，Stau、Grk等极性决定蛋白定位错误。dlg突变阻碍了Notch、JAK-STAT、EGFR等调节PFC分化的信号通路的激活。dlg突变的PFC也没有获得前端滤泡细胞命运。dlg突变不影响前端滤泡细胞群中边界细胞的分化，但是在一定程度上影响伸展细胞和向心细胞的分化，并且这种影响不依赖于前端滤泡细胞Notch或JAK-STAT信号激活的异常。

In uninduced HEL cells, GATA-2 transcription factor can specifically bind to the regulatory elements of human β-globin gene, including the proximal regulatory element (theβ-promoter) and the distal regulatory elements (the DNaseⅠ hypersensitive sites in the locus control region, HS2-HS4 core sequences).

在非诱导的HEL细胞的核蛋白中，能够与β—珠蛋白基因的近端启动子调控序列及远侧端LCR调控序列（HS2，HS3和HS4核心DNA序列）相结合的蛋白质主要是GATA—2转录因子，而在羟基脲诱导的HEL细胞核蛋白中，与上述调控序列相结合的蛋白质主要是GATA—1转录因子。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。