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Objective:To evaluate the role of telomerase activity in development of acute myeloid leukemia.

目的:探讨端粒酶活性在急性髓细胞白血病发生中的作用。

To study the effects of albumin overload on proliferation and apoptosis in proximal tubular epithelial cells.

探讨白蛋白对近端肾小管上皮细胞增殖与凋亡的影响。

To make it clear, using multilaser flow cytometry, molecular biologic technology and laser confocal microscope scan technology, we fixed eyes on mitochondrial permeability transition pores, mitochondrial transmembrane potential and caspases-3 (cysteinyl aspartate-specific proteinases) which are playing key roles of mitochondria. Meanwhile, the research also discussed the characteristics of Δψm of cells in cut specimenfrom esophageal carcinoma, adjacent tissue of esophageal carcinoma and the normal esophageal tissue.

为探讨食管癌细胞凋亡过程中线粒体所起的具体作用,本课题采用流式细胞检测、分子生物学和激光共聚焦扫描技术等手段,研究了食管癌细胞凋亡中,线粒体所起关键作用的环节—线粒体通透性转变孔(Mitochondrion Permeability Transition Pore,MPTP)、线粒体跨膜电位ΔΨm和线粒体释放反应中的Caspase-3(Cysteine protease-3);同时研究了食管癌手术切除标本中癌灶、癌旁和切端三处组织的细胞线粒体跨膜电位的特性。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子,本研究构建了带报告基因的载体并检测了其启动子,增强子和减弱子的功能,以此为基础,进一步构建了MBP异位表达载体,为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次,然后连续自交,通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C-分带,在BC2F3和BC2F3群体中,分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280,其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂, 94K280添加的是纤毛鹅观草的一对染色体D。

RESULTS: Totally 57 rats enrolled in this study, all animals completed Hot pain allergic symptom could not be observed on rats in experimental group during the whole experimental process, but continuously appeared in discharge percentage of nerve fibers at the central end of experimental rat sciatic nerve was remarkably lower than that of the control group 6.79%Sympathetic postganglion nerve fiber budding was not obvious in the slices of entire L4-5 dorsal root neuroganglion of injury side in 6 experimental rats,and basket-shaped cell could not be observed, but sympathetic postganglion nerve fiber budding was obvious in 2 rats in control groups with altogether 41 basket-shaped cells observed.

电生理实验:实验组大鼠坐骨神经中枢端的神经纤维自发放电百分数显著低于对照组(6.79%,46 7%)。③背根神经节切片荧光染色结果:实验组6只大鼠损伤侧L4-5的全部背根神经节切片中看不到明显的交感节后神经纤维芽生,没找到篮状细胞,而在对照组2只大鼠损伤侧L4-5的全部背根神经节切片中可看到明显的交感节后神经纤维芽生,共找到篮状细胞41只。

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.

WD40域,在真核蛋白质的数量发现,涵盖了包括各种功能适配器/信号转导,mRNA前体细胞骨架组装加工和管理模式;通常包含一个从11月24日生长激素肽的N残留端和西部数据肽在其C端和40残留长,因此得名WD40;之间的生长激素和WD是一个保守的核心,作为一个稳定的螺旋桨式平台,可以绑定的蛋白可以稳定或可逆性;形式与每个刀片是一个四叶片组成的几个类似结构的螺旋桨,搁浅反平行β-负债表;很少检测副本实例假设,形成了聚大结构,每个WD40重复序列形成了前三个链的一个刀片,并在未来的刀片上链,最后C -末端WD40重复完成了第一WD40重复叶片结构,创造螺旋桨的封闭环结构,对螺旋桨顶部和底部表面残留物,则建议协调与其他蛋白质和/或小配体; 7重复副本存在于相互作用这条路。

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are propos

WD40域,在真核蛋白质的数量发现,涵盖了包括各种功能适配器/信号转导,mRNA前体细胞骨架组装加工和管理模式;通常包含一个从11月24日生长激素肽的N残留端和西部数据肽在其C端和40残留长,因而得名WD40;介于生长激素和WD一个保守的核心,作为一个稳定的螺旋桨式平台,可以绑定的蛋白可以稳定或可逆,形成了一个螺旋桨式与每个刀片是一个四叶片结构组成若干链反平行的β-片;与数份检测实例假设,形成了聚大结构,每个WD40重复序列形成了前三个一个刀片和绞线的最后在未来的刀片链,最后C -末端WD40重复完成了第一WD40重复叶片结构,创造螺旋桨的封闭环结构,对螺旋桨顶部和底部表面残留的建议,配合和其他蛋白质的相互作用/或小配体; 7重复副本,目前这条路。

Two phylogenetic footprints, 35 bp and 17 bp in length respectively, were found in 5' intron sequence.

在内含子的5'端发现了两个高度保守的序列——进化印记,长度分别为35 bp和17 bp,35 bp的核苷酸片段能与HeLa细胞和鸡胚细胞抽提物中的蛋白结合,可能是一种调节元件。

In the presently mobile position technology, GPS's position method is one of the best. This method owns high accuracy less than 15 meters, and can be used with GPS chip in the mobile station. But GPS chip with the high price makes this technology difficult to popular. Therefore, the position technology of GSM communication network is attention to the people gradually. TOA or AOA is more often used among position technology, these methods need only the help of the position measuring instrument to attain time and angle for position calculation. They have good position accuracy, but they are also too expensive to popular. Every positioning technology has its bottleneck such as external environment influence, cost and positing efficiency. Therefore, the purpose of this research lies in finding out the mobile station location and does not emphasize the raise of the accuracy and it only uses existing information to finish position action.

在目前的行动定位技术中,以GPS定位技术为最佳,不仅拥有少於15公尺的高定位精确度,更只需在行动端加装GPS晶片即可使用,可惜高价格的GPS晶片使得此技术难以普及化,因此,以GSM通讯网路为主的定位技术逐渐受人们所重视,其中,又以TOA或AOA等定位技术较常被使用,仅透过位置测量仪器的帮助,便可取得定位所需的时间和角度,并且拥有不错的定位精确度,可惜也受到高价格因素的关系难以普及化,而每种定位技术都有其瓶颈存在,不论是外在环境的影响、成本和定位效能上;所以,本研究的目的在於找出行动端位置,即利用现有的资讯(细胞识别码、讯号强度等)来完成定位动作,而非强调其定位精确度的提高。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。