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Methods: The telomerase activity in breast cancer cells and MRC-5 cell line were detected semi-quantitively by TRAP-ELISA assay. Virus proliferation as- say and cell viability assay were used to evaluate the proliferation and cytolysis selectivity of CNHK300, and the results were compared with those of wtAd5. The breast cancer cells were treated with CNHK300 combining paclitaxel to evaluate the killing effect of the combined regimen.

用TRAP-ELISA方法检测乳腺癌细胞株(MDA-MB-453、SK-BR-3)和正常成纤维细胞株MRC-5的端粒酶活性;进行病毒增殖实验和细胞生长抑制实验,并与野生型腺病毒wtAd5进行对比,验证CNHK300选择性复制和选择性杀伤能力;用细胞生长抑制实验进一步考察CNHK300与化疗药物紫杉醇联合应用对乳腺癌细胞的杀伤作用;用Western blot检测腺病毒E1A在细胞中的表达。

During light adaptation, the diameter of rhabdoms was small, the arrangement of microvillus in the distal rhabdom was in disorder, and the area of the multivescicular bodies in the cytoplasm of the retinular cells was large, but the perirhabdomal vacuoles was very small, the amount of the lamellar bodies and mitochondrias were small, the pigment granules were distributed in all retinular cell.

结果显示:光适应时感杆束的直径较小,组成近远端感杆束的微纤毛排列零乱;小网膜细胞中的多囊体体积增大,膜下储泡囊体积减小,板膜体、线粒体等细胞器数量较少,色素颗粒分布于整个小网膜细胞。

Under the light microscope, the earliest TH-positivc cells at embryonic day 13 are localized at the ventral part of rostral midbrain. Later appearing TH-positive cells join the earlier ones dorsally and caudally.

最早的TH阳性细胞首先于胚胎13天在中脑吻端的腹侧出现;随后出现的TH阳性细胞从尾端和背侧逐渐加入早先出现的TH细胞。

PinX1 is an evolutionally conserved nucleolar protein that has been recently demonstrated as a telomerase/ telomere interacting factor in both the humans and the yeasts.

PinX1是近年在人和酵母细胞中发现的一个能与端粒/端粒酶相互作用的保守核仁蛋白。

Importantly, depletion of ARMS facilitated UVB-induced apoptosis in melanoma cells through inactivation of MEK/ERK pathway, which is not constitutively activated in most cases of acral lentiginous melanoma, the melanoma subtype found mostly in Asians.

更重要的是,减少ARMS在黑色素细胞癌细胞的表现,会促进紫外线B光诱发之细胞凋亡,其作用机转是透过MEK/ERK传递路径之抑制,此路径在多数亚洲人常见的肢端小痣型黑色素细胞癌并无自身活化之现象。

Objective To investigate the effects of different Leptospira strains on phagocytosis of peritoneal macrophages of guinea pigs, and explore the role of innate immune in the pathogenesis of leptospirosis.

目的 通过比较不同毒力钩端螺旋体对豚鼠腹腔巨噬细胞吞噬功能的影响,探讨固有免疫在钩端螺旋体病发病机制中的作用。

ABSTRACT]ObjectiveTo study the expression of telomerase activity in patients with acute myelocytic leukemia and its clinical significance.

摘要]目的研究急性髓细胞白血病的端粒酶活性表达情况,探讨端粒酶活性检测对AML的临床意义。

Based on the results, it has found that six distinct layers can be identified from lateral to medial in the olfactory bulbs, and the adults have more cells than the subadults. Ventricles of the adults take up larger vacuity, have richer choroids plexus, and primordial pallium and primordial hippocampus of them are more developed than the subadults, in which the primordial piriform is well-developed. Ventral to the primordial hippocampus, there is a septal area which can not be divided into medial and lateral parts in both adults and subadults, nor there is lateral limiting sulcus to separate the primordial piriform and the corpus striatum, but only in the subadults, a medial limiting sulcus can be clearly identified to separate the primordial hippocampus and the septal area. Besides, the corpus striatum of Onychodactylus fischeri is paleostriatum. The cell group that located at two sides of the third ventricle is amygdale, and shape and size of neurons within the telencephalon are poorly differentiation.

结果表明:爪鲵嗅球内细胞从外到内大致可分为6层结构,亚成体的细胞数量少于成体;成体爪鲵的侧脑室所占空间较大,脉络丛更为丰富,原始大脑皮层和原始海马都比亚成体发达,后者大脑内原始梨状区较为发达;隔区位于原始海马的下方,成体和亚成体都没有外侧隔核和内侧隔核之分,也没有外侧界沟作为原始梨状区和纹状体的分界,而在亚成体的侧脑室壁有内侧界沟区分原始海马和隔区,成体则没有;此外,爪鲵的纹状体属于古纹状体,杏仁核是位于第三脑室两侧的两个细胞团,端脑内细胞形态和大小分化较为单一。

Study shows that strengthening body resistance and archaeus herbs have many effects,such as improving and adjusting immunity function,protecting bone marrow,improving haematogenesis function,raising digest and absorb function,improving substance metabolism,preventing gene mutation,inhibiting tumor cell proliferation,inducing tumor cell differentiation or apoptosis,resisting tumor invasion and metastasis,inhibiting formation of tumor vessel and activity of telomerase,etc.It's possibly the result of synergistic effect by multi-pathway and multi-target.

研究表明,扶正培本药物具有增强和调节机体免疫功能、保护骨髓及提高机体造血机能、增加消化吸收功能,改善物质代谢、阻止基因突变、抑制肿瘤细胞增殖、诱导分化、促使肿瘤细胞凋亡、抗肿瘤侵袭与转移、抑制肿瘤血管形成及肿瘤细胞端粒酶活性等多种作用,其发挥的扶正作用可能是多途径、多靶点联合协同作用的结果。

Follicle-stimulating hormone receptor is a G-protein-coupled receptor,which is mainly expressed on sertoli cells of the testis and granular cells of ovary.

卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)是一种具有七个跨膜结构的G蛋白耦联受体,主要分布于睾丸支持细胞和卵巢颗粒细胞,其N端拥有一个大的细胞外结构域,用于结合FSH。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。