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DMBQ, ferulic acid and resorcin were effective chemical signal substances in the process of seed germination and haustorium formation of Cistanche deserticola. 4.The embryo is consist of 12 cells, the endosperm cell degraded so as to provide nutrition for the embryo development .As a result, germ tube differentiated from the embryo and came out through micropylar end, the small cell at the tip of the germ tube held the capacity of cleavage and the other cells grew big ,so the germ tube elongated .The anterior extremity of germ tube swelled to form haustorium under chemical substances treatment, the haustorium cell stained ,especially its portrait because of the lignification of the cells.

肉苁蓉种子的胚是由12个细胞紧密排列形成的圆球状胚,球形原胚分裂增殖的同时,胚乳细胞分解,为胚细胞的增殖提供营养,最终胚上分化出的芽管由珠孔端冒出,芽管前端细胞较小,具有旺盛的分裂能力,后部细胞体积增大,芽管逐渐伸长,在化学物质的诱导下,芽管由中部至前端细胞膨大分化出初生吸器,初生吸器呈帽子状,而这些细胞比其周围的细胞着色深,吸器纵向局部区域的番红染色加深,细胞高度木质化。

Results The hind limb function of the injured rats recovered at different degrees, the most extent recovery occurred during the time site 1~2 week, recovery continued from 2 to 3 week and, BBB was up to 12 at the end of the third week, but there was no significance recovery during 3~4 week. the astrocyte caudal to the injury plane began hyperplasy and hypertrophy; astrocyte in which GFAP in expression was positive the gray matter increased obviously from 3 days to 14 days after hemisection. The expression of MBP is same as that of GFAP.

结果 伤后后肢均有不同程度的恢复,1~2周时恢复幅度最大,2~3周时后肢运动功能继续恢复,3周时BBB评分最高达12分,3~4周运动功能无显著性恢复,损伤后1 d损伤远端3~6 mm处GFAP阳性星形胶质细胞开始增生肥大,3~4 d灰质中星形胶质细胞明显增多,2周时达到高峰,损伤近端3~6 mm处少突胶质细胞的增生反应过程与星形胶质细胞相似。

Deserticola showed polarity, with the micropyle cells being smaller than the chalazal ends and differentiated into white radicle-like organs.

肉苁蓉种胚具有明显的极性,珠孔端细胞小于合点端,珠孔端细胞分化、生长并产生白色的类胚根状结构。

There were two or more nucleole in the nuclei of the apicalmeristem cell when the plant went into reproductive phase from vegetative phase.

植株由营养生长转入生殖生长时,茎端细胞的核内有2个或多个核仁。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡,原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化:细胞核固缩,核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘,并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用,与对照组相比有显著性差异(p<0.01),在剂量为0.8-8μmol/L范围内,抑制率随剂量的增加而增加,当剂量超过8μmol/L时,抑制率反而下降;3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性,引起DNA降解损伤,反向调节细胞周期活动,促使细胞从G0期进入G1期,抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核,可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

The results showed that:(1) Many proliferating cells were distributed unevenly in the ventricular zone of telencephalon, and the [3H] labeled cells were found to be populated in the lateral ventricular zone of neostriatum at brain level L12~L14,forming proliferation "hot spots";(2) Having underwent mitosis twice, the proliferative cells in the VZ began to migrate laterally 6 days after intramuscular injection of [3H] thymidine, and these migrating precursor cells terminated at their targets and differentiated into new neurons on the 30th day.

结果发现:①端脑室带区中存在不均匀分布的[3H]标记细胞,大量标记细胞分布在端脑尾侧新纹状体水平(L1~L14)的VZ,形成标记细胞的&热点&区;②标记细胞经两次有丝分裂后,大约在肌肉注射[3H]的第6天后开始从VZ向外迁移,在30天左右到达目的脑区并分化成为成熟神经元。

Results C/EBPαand C/EBPβexpressed differently in the outer root sheath,follicular papilla,hair matrix and sebaceous gland in embryonic period.The positive signals were chiefly distributed in the nucleˉuses.The expression of C/EBPαand C/EBPβwere found in the basal cell layer of outer root sheath and sebaceous gland in the new born and maturation phase,The expression in nucleuses of outer root sheat cells decreased with the differentiation but enhanced oppositely in the cytoplasm.

结果 C/EBPα和C/EBPβ分别在胚胎期毛囊外根鞘,毛乳头,毛母质及皮脂腺细胞的胞核内强表达;新生儿毛囊峡部下端外根鞘基底层细胞的胞核内也存在强表达,基底内层胞核阳性信号逐渐减弱;而成体毛囊核内阳性信号仅定位于峡部下端外根鞘基底层及皮脂腺基底层。

Telomerase activation is a key tumor marker which immortalizes cell.If a medicine blocks telomere prolonging by attenuation of telomerase activity, it could inhibit tumor cells proliferation.

因为端粒酶活化是大多数肿瘤细胞维持端粒长度并获得永生化的必要条件,如果某种药物能抑制端粒酶的活性就可阻断端粒的合成,从而使细胞退出增殖周期,达到抑制肿瘤细胞增殖的目的。

The telomere and telomerase play an important role in the malignant progress of tumor cells and the mainten.

端粒—端粒酶系统在肿瘤细胞的恶性进展和永生细胞的维护中起重要作用。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。