端细胞

AIM: To investigate the significance of the expression levels of human telomerase reverse transcriptase gene in peripheral blood mononuclear cells of lung cancer in elderly patients.

目的：探讨外周血单个核细胞的人端粒酶反转录酶基因的表达水平对于老年肺癌的诊断意义。

Elemene may suppress telomerase activity through inhibiting expression of hTERT pro- moter of gastric cancer cell.

榄香烯乳可能通过下调hTERT启动子活性，抑制hTERT表达，从而抑制胃癌细胞端粒酶活性。

It had a good radiopacity almost equivalent to that of a 2 mm thick aluminium wedge when the iodine content was 3.5 wt%.

MTT法测试表明对碘苯胺封端的聚氨酯材料不具有细胞毒性。

The measurement of telomere length can give an insight intothe replicative history of the cells in question.

端粒酶长度的测量技术给正在研究中的细胞的复制史提供了一个新角度。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

In conclusion , ERK and telomerase serve a function to some extent in drug resistance of leukemia and ovariancarcinoma cells. The inhibition of ERK signal transduction pathways led to reduction of phosphorylated ERK1 and ERK2protein expression level , and successionally down2regulated the telomerase activity.

通过阻抑ERK通路，降低磷酸化ERK1/ 2蛋白表达水平，进而下调端粒酶活性，可以达到增强白血病和卵巢癌耐药细胞对HRT 或DDP 敏感性的目的。

One of the 17 positive clones we have obtained encoded the glutamine-rich carboxyl terminus of TIAR, and this interaction between tTG and TIAR, which was finely regulated by Ca 2+, was proved in vitro by GS...

序列测定显示其中 1个克隆所含cDNA序列编码TIA 1相关蛋白(TIA 1 relatedprotein ，TIAR )C端 12 9个氨基酸残基序列，GST下拉实验也证实tTG与TIAR能相互作用，而且这种相互作用需要Ca2 +参与作用。这些结果提示tTG可能通过其Ca2 +依赖的转谷氨酰胺活性对TIAR进行修饰从而影响TIAR的功能，可能在细胞凋亡中发挥着一定的作用。

Then glutamate, GABA and GABA_BR1 in cells on bone trabecula surface of distal fermur of rats in every group were checked with immunohistochemistry method.

运用免疫组织化学方法检测各组大鼠远端股骨骨小梁表面细胞的Glu、GABA和GABA_BR1。

Methods DNA aneuploid and TA were detected respectively in 165 cases of malignant pleural effusion and 127 cases of benign dropsy of chest (benign group, including tuberculous,other effusion and transudate of chest) by FCM and TRAPHybELISA.Carcinoembryonic antigen was also detected in the two groups.The difference of DNA aneuploid and TA between these two groups was analyzed.

恶性积液165份、良性积液127份(包括结核性、其他渗出液、漏出液)，应用流式细胞术检测DNA异倍体、端粒末端重复序列扩增—杂交—酶联免疫分析技术检测TA，同时检测临床常用的癌胚抗原，比较各类积液中上述3项指标检测结果的差异。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。