端细胞

Water, Glycerin, Methylsilanol Mannuronate, Sodium Carboxymethyl Beta-Glucan, Sodium Hyaluronate, Palmitoyl Oligopeptide, Palmitoyl Tetrapeptide-7, Madecassoside, EGF *, FGF *, Telomerase *, Panthenol, Borago Officinalis Seed Oil, Anthemis Nobilis Flower Extract, Tocopherol, Tocopheryl Acetate, Cetyl Palmitate, Citrus Aurantium Dulcis Peel Oil, Citral, Citronellol, Limonene, Linalool, Zea Mays Starch, Butylene Glycol, PEG-40 Hydrogenated Castor Oil, Hydrolyzed Corn Starch, Hydrolyzed Corn Starch Octenylsuccinate, PEG-33, PEG-8 Dimethicone, Hydroxyethylcellulose, PEG-14, Xanthan Gum, Carbomer, Polysorbate 20, Caprylyl Glycol, Phenoxyethanol, Methylparaben.

主要有效成分是：聚羧基甲基β葡萄糖的钠盐，保湿锁水；透明质酸，高效保湿；寡胜肽；四胜肽；羟基积雪草甙，抗炎症，促进胶原蛋白的合成；表皮生长因子EGF，促进细胞新陈代谢；成纤维细胞生长因子FGF，促进胶原蛋白合成；端立酶，一种RNA与蛋白的复合体，可通过合成新的端粒而修补受损的DNA端粒，使细胞持续不停的增生，也就是利用成人干细胞转变为全新的细胞。维他命B5；琉璃苣籽油，含维他命和矿物质；洋柑橘萃取液，舒缓，抗过敏；维生素E；醋酸盐维生素E；橙皮油；水解玉米淀粉。

Our aim was to investigate the effect of malate and GABA on telomerase activity of Hela cells and ex-plore the relationship of telomerase activity change with growth, metabolization and deterioration of cancer cells to provide new tactics for treatment of malignant tumor.

Hela细胞是研究端粒酶的常用细胞，本研究旨在观察苹果酸和GABA这两种作用因素对Hela细胞端粒酶活性的影响，探讨端粒酶活性的变化与二者在肿瘤细胞生长、代谢和恶化方面作用关系，为恶性肿瘤的治疗提供新的策略。

Deserticola showed polarity, with the micropyle cells being smaller than the chalazal ends and differentiated into white radicle-like organs.

肉苁蓉种胚具有明显的极性，珠孔端细胞小于合点端，珠孔端细胞分化、生长并产生白色的类胚根状结构。

The significant changes were obseved in the morphology,size,number and the degree of development of nuclei,nucleole,mitochondrial and chloroplast of apicalmeristem cell.

在这期间茎端细胞内核仁、线粒体、叶绿体等的形态、大小、数量、发育程度等发生明显变化。

There were two or more nucleole in the nuclei of the apicalmeristem cell when the plant went into reproductive phase from vegetative phase.

植株由营养生长转入生殖生长时，茎端细胞的核内有2个或多个核仁。

The main components of every ommatidium of compound eye were completed in the larval stages. An ommatidium composed of cornea, four corneagenous cells, four cone cells and retinular cells. The sensitization system of an ommatidium included 11 retinular cells, of which four located at the distal part of the ommatidium; seven formed the proximal main part of the retina. Their structures are different.

小眼的感光系统由11个小网膜细胞组成，且与成体一样，11个小网膜细胞已经分化成4+7两层，其中4个小网膜细胞构成了感光部分的远端，7个小网膜细胞构成感光系统的近端主体，远端和近端的小网膜细胞在结构上也有明显差异。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

These results revealed that the pollen chamber developmental pattern passed through four phases. Firstly, a group of nucellar cells at the micropylar end elongated longitudinally. Thereafter, the uppermost nucellar cells at the micropylar end initiated PCD; and these elongated cells undergoed PCD in a basally and laterally oriented direction to form a cavity.

贮粉室的形态发生可分为4个阶段：最先是位于珠孔端的几层珠心细胞纵向地伸长；接着，珠孔端最顶部的珠心细胞发生PCD；然后，那些已经纵向伸长的珠心细胞向基地和侧向地依次发生死亡，产生贮粉室空腔；最后，珠孔端的珠心表皮细胞发生开裂，形成贮粉室的开口。

Telomerase activation is a key tumor marker which immortalizes cell.If a medicine blocks telomere prolonging by attenuation of telomerase activity, it could inhibit tumor cells proliferation.

因为端粒酶活化是大多数肿瘤细胞维持端粒长度并获得永生化的必要条件，如果某种药物能抑制端粒酶的活性就可阻断端粒的合成，从而使细胞退出增殖周期，达到抑制肿瘤细胞增殖的目的。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。