端出

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Note that the receiving end is located at x = 0; the sending end is at x =l

注意接受端被找出在x = 0；送的末端是在x = l

I don't profess to understand it, but apparently, done properly, if one hits the bottle right under the lip of the cork at the bottle's weakest point, on a well-chilled bottle, the cork and glass fly away, spilling little and leaving a rather smooth break.

虽然我不能说在理论上理解这一技法，但毫无疑问能够正确的运用它。一瓶彻底冰镇过的香槟，只要找到瓶子最脆弱的地方，沿软塞底端一刀切下，软木塞和玻璃会连同一点点液体飞射而出，留下一个相当光滑的切口。

A wedding guest told the New York Times that he had shown no sign then of being extremist or even at all religious.

一位参加婚礼的宾客告诉纽丨丨约时报，当时他没有表现出任何极'端分子甚至宗'教主义的迹象。

In chapter 3, the FLUENT software is used to simulate the 2D and 3D fluid fields of three kinds of distribution pairs (cylinder block and piston, valve port and end cap, valve plate and cylinder block).

第三章，利用Fluent流场仿真软件，对液压变压器三个关键配流副(配流盘-后端盖，配流盘-缸体，缸体-柱塞)的流场进行了初步的二维和三维仿真分析，给出了配流盘控制角度为60°下的仿真结果。

Tilt-out rock, covered with three adjacent caves: the left side of Hope caves, the middle is the wind tunnel, Fuxi is the right hole.

岩端倾斜而出，覆盖着三个毗邻的岩洞：左边灵岩洞，中间是风洞，右边是伏羲洞。

The hollow-axis motor drives the offset nozzle and rotates the arc on the tip of wire at high speed,so as to ensure enough penetration into the sidewalls in narrow gap welding .

在该系统中，焊丝穿过电机的空心轴后从导电嘴的偏心孔送出，电机带动导电杆和偏心导电嘴旋转，从而使焊丝端部的电弧以一定直径高速旋转，以保证窄间隙焊接接头具有足够的侧壁熔深。

Except for the acral-lentiginous subtype of melanoma, the vast majority occurs in sun-exposed areas.

除肢端雀斑痣样黑素瘤外，大多数黑素瘤出现在阳光照射部位。

Ensure that the suction valve and the leakage discharge hole alignment, hydraulic end of the screw and the corresponding four holes aligned.

确保吸液阀与漏液排出孔对齐，液力端的螺丝与相应的4个孔对齐。

New plantlets, borne on the end of long strap-shaped leaves, take root easily, hence "walking" iris.

长在长块状的叶端的新出的小植物，很易就能生根，由是英文名 'walking'会走路的

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。