端出

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

It is indicated that, the bending curve is not asymmetrical and there will not have moment of flexure on the top of the bending section. Based on a slantwise constant-thick beam which foot is fixed, and took deadweight of bending section to account, a theoretical differential equation of third order for buckling equilibrium state was established.

在分析了顺层斜坡地质原型变形形态的基础上，由考虑自重的阻滑端固定的斜置等厚弹性板梁模型，提出了以地质原型变形形态抽象出相应边界条件的分析方法，及理论上求解挠曲线的适定三阶微分方程。

The neurosecretory somata were located onthe proximal surface of the medulla terminalia of the eyestalk.Three types ofneurosecretory cells were distinguished on the basis of size, morphology,distribution,form of outgrowth and ultrastructural feature.

河蟹眼柄神经分泌细胞主要分布于眼柄视神经节端髓基部外侧，依据细胞的大小、形态、超微结构特征及离体生长形态区分出三种类型神经分泌细胞。

Squeege the beginning of the movement and Terminal should be at least a Scripture than printing plate to produce 10-15cm business card printing and membership card.

刮板活动的始端和末端起码答比印邦畿不白长出10-15cm才能制卡和会员卡制作。

Firstly, a homogeneous Harnack inequality as well as a maximum principle of nonhomogeneous subelliptic p-Laplace equations is proved by improving the classical Moser iteration method. The optimal estimate of m is also obtained through log |log| method.

首先改进经典的Moser迭代方法，证明了非齐次次椭圆p-Laplace方程的极大值原理和齐次Harnack不等式，并利用log |log|方法给出了不等式右端指数m的最佳上界估计。

Methods DNA aneuploid and TA were detected respectively in 165 cases of malignant pleural effusion and 127 cases of benign dropsy of chest (benign group, including tuberculous,other effusion and transudate of chest) by FCM and TRAPHybELISA.Carcinoembryonic antigen was also detected in the two groups.The difference of DNA aneuploid and TA between these two groups was analyzed.

恶性积液165份、良性积液127份(包括结核性、其他渗出液、漏出液)，应用流式细胞术检测DNA异倍体、端粒末端重复序列扩增—杂交—酶联免疫分析技术检测TA，同时检测临床常用的癌胚抗原，比较各类积液中上述3项指标检测结果的差异。

At last, the analysis as to the constructive relation of the bond average strength and load-end slip at the decreasing state results in the conclusion: it can be described trinomially.

最后，对平均粘结应力与加载端滑移在下降段的本构关系进行了分析，给出可用三次多项式进行描述的结论。

The agitation power consumptions in boiling systems decrease uniformly with the increase of agitation speed, whereas the relative power demand (RPD-the ratio of agitation power input of HEDT for gassed system to that for ungassed system and under the same agitation speed) can maintain at a high level for high gas loadings even when the impeller is operating with a high impeller tip speed. The gas hold-up in the boiling system is much lower than that in the cold gassed system, and the gas hold-up in boiling system increases at first and then decreases with the increase of the agitation power consumption per liquid mass P(subscript m. It implies that the boiling three-phase system has a maximum gas hold-up point. The changes of nucleation site of vapour bubble and the character of suspension of the solid particles with the rotational speed of the impeller are responsible for those behaviors mentioned.

在沸腾条件下，搅拌功率消耗随着搅拌转速的增加有所下降，但HEDT桨具有较高的载气能力，即使在高叶端线速度下，相对功率消耗仍能保持在较高的水平；沸腾体系的气含率随单位质量流体输入功率的增加表现出先增后减的规律，即存在气含率的极大值，蒸汽成核位置和固体颗粒悬浮特性随搅拌功率增加而改变是造成上述行为的主要原因；沸腾体系中的气含率远远低于常温通空气体系。

Hepatitis B virus is the smallest DNA virus which is a member of the viral family Hepadnavirida. It uses its DNA as template and the RNA polymerase II of the host cells to transcribe its viral transcripts. There are four major RNAs transcribed from HBV genome. All of the four major transcripts are unspliced, terminated at a common site, polyadenylated at the 3' terminal and capped at the 5' terminal.

B 型肝炎病毒属於肝病毒科，为目前已知最小的去氧核醣核酸病毒，利用其病毒的去氧核醣核酸以及宿主细胞内的核醣核酸聚合酶可转录出四种主要的核醣核酸产物，此四种主要的转录产物均未经剪接并终止於核心开放编阅序列的共同polyadenylation 讯号，且在其5'端均经过capping修饰。

Friends still, the new Friends of the layers of meaning of life is to transform multiport.

友人依旧，新友层出，生命的意义在于变换多端。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。