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Primers were designed to amplify a 264 bp region within the lipL32 gene that is conserved among pathogenic Leptospira and absent in nonpathogenic species.

引物设计选择了 lipL32中一段长为264bp的碱基，该区域存在于致病性钩端螺旋体中，而非致病性钩端螺旋体无此区域。

Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.

用三种不同毒力的钩体（致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株）分别感染体外培养的豚鼠腹腔巨噬细胞，并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min，通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数，检测钩体对巨噬细胞吞噬功能的影响；感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化；用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。

Returns the value of a request parameter as a String, or null if the parameter does not exist.

获得客户端传送给服务器端的参数值，该参数由name指定，通常是表单中的参数。

Though of high station, one must be kind and forgiving.

虽贵端，慈而宽虽然自己是贵端的，但是要仁慈和宽容。

12 In dealing with maids and servants, one is of high station. note 12

待婢仆，身贵端对待侍婢和仆人，自己是贵端的。

12 In dealing with maids and servants, one is of high station.

待婢仆，身贵端对待侍婢和仆人，自己是贵端的。

In the two-phase activation model, the r esources for a "call" are first "admitted,"and then once the end-to-end negotiation is completed (e.g., called party' gateway generates an s "off-hook" event), the resources are activated.

在这个两阶段激活模型中，一个呼叫的资源首先被接纳，一旦完成端对端协商，资源被激活。

He has to be dominant on offense and especially on defense.

他需要在进攻端的，尤其是防守端的具有统治力。

Some one officiously took the dish from Bagration (or he would, it seemed, have held it so till nightfall, and have carried it with him to the table), and drew his attention to the poem.

有个人怀有奉承的心情拿走巴格拉季翁手里的银盘（要不然，他好像就要这样不停地端到晚上，并且端着银盘上餐桌），这个人请他注意那首诗。

The sensitization system of an ommatidium included 11 retinular cells, of which four located at the distal part of the ommatidium; seven formed the proximal main part of the retina. The two parts are some one on top of another.

透射电镜观察显示红螯螯虾小眼的感光系统由11个小网膜细胞组成，其中4个小网膜细胞位于晶锥之下，构成了感光部分的远端，7个小网膜细胞构成感光系统的近端主体，上下两部分有重叠。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。