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We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

Group one consisted of 300 patients whose esophageal stumps were treated by a purse stitch using a clamp. Group two included 180 patients whose esophageal stumps were oversewn by 6-8 interrupted stitches, or by a continuous stitch along the stump before the anvil was in place.

第一组300例，食管残端用荷包钳法作荷包处理；第二组180例，食管残端在荷包钳处理的基础上，再间断全层缝合食管壁并结扎缝线，或沿食管残端连续全层缝合一周，放入抵钉座后结扎缝线。

When we are photographed through the digital cameras,, we become information, signal and code in the process of decoding and recoding into 0 and 1. After the restructuring and re-existence, the portrait of whole has become anyone but on one.

个人原有的肖像，见证了曾出现於世界此端的真实，由网路的此端传输到世界的彼端，透过数位编码，让个人的真实存在以讯号重组，个人在讯号流动之中，失去了存在的唯一性，被解码与重新编码成为0与1，在与众生重组的过程中换成另一个不同面貌重新存在，成为在场又不在场的他者。

The main work of this paper: Firstly, Basing on the summarization and analysis of the walking mechanism, Parallel drive five-bar mechanism is applied for walking mechanism. And then the characteristic of the trail and positive kinematics as well as reverse kinematics are discussed. Secondly, the model of walking mechanism is established according to Parallel drive five-bar mechanism, and then a figure planning is designed for the foot-end trail. The characteristics of the parallel drive are emphasized to research in this paragraph by the method of insert value. At last, the simulation is performed in the ADAMS to compare the real result to the theoretical value. Thirdly, a sealed structure of a Quadruped Walking Robot is designed and established. The whole control system, including motor, sensor and control circuit, is sealed in the robot. Walking is derived in the way of the shaft driving and the dynamic torque sensor is used to the surrounding conditions of the robot's foot-end to be the foundation of the robot's control. At last, the applying principle of the dynamic torque sensor is researched in detail basing on the traditional sensor of the walking robot. Moreover, the principle is divided into several sorts to discuss. Finally, simulation and verification of the monitor principle are come through in the Mechanism of the Pro/Engineer.

论文的主要工作有：在对传统步行机构总结分析的基础上，提出将平面并联五杆机构应用于四足步行机器人的腿部结构，并对其轨迹特性、正运动学、逆运动学特性做了详细计算分析；依据平面并联五杆机构的模型，建立机器人步行机构的实体模型，对其足端的轨迹做了图谱规划，并利用插值法对并联驱动端的特性曲线作了研究，并在ADAMS中进行仿真，将结果与理论值相比较；然后设计了一套四足步行机器人密封装配结构设计方案，建立四足步行机器人单足结构、驱动以及传感器监测的三维实体模型，将机器人的整个控制系统，密封装配在步行机器人的机体中，通过轴传动来驱动步行机构实现步行，采用动态扭矩传感器监测步行机构的驱动关节受到的扭矩变化，从而获得四足步行机器人足端的周围情况，为步行机器人的控制方案奠定了基础；论文的最后在分析步行机器人传统控制传感器的基础上，详细分析了动态扭矩传感器的监测原理，并分几种情况分别分析了其监测原理，并在Pro/E的Mechanism模块中实现了监测原理的仿真与验证，最后给出了传感器监测信息与系统判断之间的关系。

The thesis proves the method and gives the Path Construction Algorithm Based On Trust Certificate Tree.

另外，CAClient采用端到端的传输模式，系统可以发起和监听对等端的连接请求，建立多任务的对等文件传输。

The indoor model test using organic glass is carried out and the displacement, stress, earth pressure as well as its distribution type are measured to verify the theoretical values.

使用有机玻璃进行室内模型试验，实测了端墙的位移、路面板应力、端墙应力及土压力，验证了理论分析结果，获得了端墙背后土压力的分布形式。

The invention relates to a desk type drill machine for preventing drill head wreckage, comprises chassis, supporting post, operating board covered in middle of supporting post, cabinet and generator; cabinet is equipped with tension pulley pressure sensor, belt tension unit and pressure foil gage; tension pulley is compacted on driving belt of drill machine, pressure foil gage is installed on top bar of tension pulley, the pressure foil gage is in electric connection with input end of controlling circuit, output controlling end of controlling circuit is connected to power input end of generator.

本发明是防止钻头折断的台式钻床，包括底座、支柱、套在支柱中部的工作台、以及机箱和电机，机箱上设有张紧轮压力传感器，由皮带张紧装置和压力应变片组成，其中的张紧轮紧压在钻床的传动皮带上，压力应变片设在张紧轮的顶杆中，压力应变片与控制电路的输入端电连接，控制电路的输出控制端连接在电机的电源输入端中。

A fan frame includes a body and a fan wire fixed on the body. The body is provided with a fixing spring plate which forms a free end. A fixing groove, through which the fan wire passes, is formed between the fixing spring plate and the outer surface of the body. The outer surface of the body protrudes outward to form at least one stop dog. The top end of the stop dog extends in the side direction and exceeds the free end of the fixing spring plate. The stop dog and the fixing spring plate form a space to contain the fan wire in the side direction. A guidance surface is gradually formed from the bottom end to the top end of the stop dog.

一种扇框，包括一本体及固定在本体上的风扇导线，该本体上连接设有一固定弹片，该固定弹片形成一自由端，所述固定弹片与本体的外表面之间形成供风扇导线穿设的一固定槽，该本体的外表面向外突设形成有至少一挡块，该挡块的顶端在侧视方向上延伸超过该固定弹片的自由端，该挡块与所述固定弹片在侧视方向上形成一个收容风扇导线的收容空间，且该挡块的底端至顶端逐渐形成一导引面。

Setting up a port forward rule to forward UDP port 1194 from the firewall/gateway to the machine running the OpenVPN server.

设置一个端口转发规则，将UDP端口1194从防火墙/网关转发到运行OpenVPN服务端的机子。

In the computer chassis before and after the panels are left in USB interface, we should, if possible, mobile hard drive inserted into the chassis back panel USB port, after all, the back panel of the USB ports are integrated directly into the computer motherboard, this USB port are generally considered safe grounding design, we found the body of the static it becomes hard to move a threat to the safety of the hard disk USB interface.

在电脑机箱前后面板中都留有USB接口的情况下，我们应该尽量将移动硬盘插入到机箱背面板上的USB端口中，毕竟背面板上的的USB端口通常直接集成在计算机主板中，这种USB端口一般都有安全接地的设计考虑，所以我们人体身上的静电就不大容易威胁到移动硬盘USB接口的安全。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。