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Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测,同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

Objective To analyse the mutation types of the NOTCH3 gene with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy.

目的 分析伴皮质下梗死及白质脑病常染色体显性遗传性脑动脉病的NOTCH3基因突变类型。

All these results demonstrate that mutation does not cause substantial influence on the function of prochymosin and pseudochymosin with respect to autocatalytic activation.

这些结果表明突变对凝乳酶原的自我活化功能影响不大。

Objective To study the role of mLT63, an avirulent mutant of heat-liable E. coli enterotoxin, as an adjuvant in intranasal immunization and compare with that of CpG-ODN.

目的 研究大肠杆菌不耐热肠毒素无毒突变体mLT63和CpG-ODN鼻内免疫对抗原的佐剂效应。

The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变;沉淀回收的PCR产物以20μl变性上样液(95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝)溶解后热变性为两条DNA单链后,上样于8%非变性聚丙烯酰胺凝胶(含5%甘油),700伏电压、4℃电泳16小时。

According to evolutionists, random mutation is purportedly what brought life from the one celled stage to its present complexity.

据evolutionists,据称是随机突变带来什么从一个细胞生命阶段,到现在的复杂性。

Seedlings of these mutants subjected to chilling stress became chlorotic and had half the chlorophyll content of wild-type.

这些突变种的幼苗株在遭受低温胁迫时,比起野生型,叶绿素含量少了一半。

In 7 cases point mutation was found positive whereas negative result was found in the cytological examination.

结果显示有7例首先在纤维文气管镜刷检的脱落细胞中发现H-ras基因点突变。

Chromosomes of the desynaptic variant at metaphase I,24 I

突变株的花粉母细胞在中期I形成24个单价体

Pachytene chromosomes of the desynaptic variant; C.

突变株的花粉母细胞在粗线期同源染色体配对正常;C。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。