突变的

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Our three groups in SCBB will conduct one project in this grant (three projects in this grant total), which is "Screen mutants interfering the development or regeneration of endodermal organs and study their functional mechanisms". In this project, stem cell and zebrafish will be used as models to elucidate the mechanisms of the development of endodermal organs.

我院的三个课题组负责此项目的课题三《正向遗传突变筛选具有内胚层器官发育或再生缺陷的突变体及致变基因的功能机制研究》，将采用干细胞和斑马鱼模型，研究内胚层器官的机制，特别是分子调节网络的整体性和时空性，为防治肝脏和胰腺的重大疾病提供坚实的理论基础。

Conbining previous results with our results, we conclude that the soluble gp130 mutant containing CBD can antagonize the biological activities of IL-6 by competitively binding IL-6/IL-6Rα with wild-type gp130, and the membraneanchored mutant could transduce IL-6 signal, but its efficiancy was much lower than that of wild-type gp130, therefore, we thought the Ig-like module also played an important role in IL-6 signal transduction by involving in the formation of a hexameric IL-6 receptor complex.

结合以前的研究结果，我们认为只含胞外CBD区的gp130突变体通过与膜结合型gp130竟争结合IL-6／IL-6Rα复合物而具有拮抗IL-6功能的作用；而只含胞外CBD区的gp130突变体，尽管仍能传导IL-6信号，但其效率远低于野生型gp130，因此，我们认为gp130的Ig样区在IL-6转导中也起着十分重要的作用。

He SPRY domain of TRIM5α, which may be responsible for recognition of incoming viral capsids showed higher nonsynonymous/synonymous substitution ratios than the non-SPRY domain, indicating that the adaptive evolution of TRIM5α in primates might be an innate strategy developed in defending retrovirus infection during primate evolution.

RIM5α的 SPRY 结构域可能负责识别入侵病毒的衣壳，这个结构域比基因的其它部分在进化过程中具有更高的错义突变同义突变比率，表明 TRIM5α的适应性进化可能是灵长类发展抗反转录病毒感染策略的结果。

It was found that PCBA-BPy and PCCA-BPy exhibited both smectic and nematic phase while all of their predecessors showed no smectic phase.

结果表明，这两个氢键复合物都具有由于分子间氢键作用而导致的近晶相，但它们中存在的氢键作用却不相同，在PCBA-BPy中，温度变化时，发生氢键结合的羰基的吸收峰的位置仅在发生晶型转变时有突变，而在PC-CA-BPy中，温度变化时，其羰基的吸收峰的位置则基本不发生突变。

In this research it was established that a method of isolation of total RNA from Ziziphus jujuba Mill. cv. wuhexiaozao and Ziziphus jujuba Mill. cv. jinsixiaozao fruit which were from different grown time. Also the Reverse Transcription System for Chinese jujube fruit was established in the research. The purpose of this research was to lay a solid foundation for cDNA-AFLP analysis, to illustrate the mechanism of stoneless of Chinese jujube, to clone the gene to stoneless, to cultivate new Chinese jujube cultivars by gene modified breeding.

以无核小枣和金丝小枣不同发育时期的果实为试材，建立适宜枣果实总RNA提取的方法及其反转录的技术体系，为进一步进行枣果实cDNA-AFLP分析及有关的分子生物学操作，阐明枣无核突变体的分子机理，并克隆枣果实无核突变体相关基因，通过基因工程培育优良的无核枣品种奠定基础。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

We give all of these properties by means of theorems, and also give detail proofs. In the most important theorem, we give the relation between singularity and jumps and sharp cusps neighborhood. and the threshold detection of jumps and sharp cusps, and the relation between threshold and scales, where we partly resolve an open problem put forward by some researcher.

在最关键的定理中，给出了奇异性与突变点和尖点的领域的关系，以及突变点和尖点的阀值检测法，以及阀值和尺度的关系，这部分的解决了以前研究者提出的一个公开问题，同时研究了逼近程度和逼近理论，这在已有的基础上又向前进了一步。

Firstly, we study the theory and application of jump points and sharp cusp points detection by wavelet transform. The studied object is from white noise to correlated noise. We have studied the properties of correlated noise in wavelet transform, and the properties of deterministic function with jumps and sharp cusps in wavelet transform.

首先研究了研究了小波变换在突变点与尖点检测中的理论与应用，研究的对象由白噪到相关噪声，研究了相关噪声在小波变换的性质，以及对具有突变点和尖点的函数在小波变换下的性质，有关这些性质，我们均以定理形式给出，并给出了详细的证明。

The main conclusions are those:(1) the cause of hot-dry climate before elevation of plateau is that the efficiency of atmospheric heat engine is lower than after the elevation owing to flatter global landform;(2) at the beginning of Oligocene Epoch,the horizontal scale of plateau reached the cr...

主要结论：（1）高原隆起前的干热气候是由于隆起前全球地势平坦，致使大气热机效率很低之故；（2）渐新世初高原的水平尺度达到了斜压大气地转适应的临界尺度，大气环流从南北两极&两涡对峙&突变成地球三极的&三涡鼎立&，气候发生突变；（3）分析了2.5Ma时高原抬升到2000m这一重要临界高度后大气环流的一系列相应变化；（4）计算、比较了最大冰期地气系统能量平衡与现代的差异，并由此分析了当时的气候状况

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。