突变的

Quantitative RT-PCR results showed that HTF1 is involved in the conidiation pathway,Htf1 mutant is not de-repressed in conidiophore development under non-conducive conditions.The observed phenotype of enhanced conidiophore development in the htf1 mutant may result from its defect in conidium development.It is possible that some feedback signal from conidia may inhibit further conidiophore development in M.grisea.

定量RT-PCR的结果也表明HTF1参与产孢信号途径。htf1突变体在不利于产孢条件下并没有去抑制分生孢子梗的发育，因此htf1突变体产生比野生型菌株数量更多的分生孢子梗可能是由于其无法形成分生孢子的原因导致的，这也似乎预示了在稻瘟病菌中很有可能存在一些来自分生孢子的反馈信号以进一步抑制分生孢子梗的发育。

As shown by the result, the biomass and astaxanthin content of oceanic red yeast was reduced due to the presence of β-ionone which inhibited the synthesis of carotenoids. The lethality rate of oceanic red yeast would attained 92.3% if 370 mg/L of the β-ionone concentration was contained in the plate medium. The oceanic red yeast mutated by ethyl methane sulfonate was cultured on plate medium containing 370 mg/L of the β-ionone. 200 mutants were randomly screened, positive mutants with increased the biomass, astaxanthin volumetric yield and astaxanthin specific yield accounted for 18% of the total strain, the mutants with higher biomass, astaxanthin volumetric yield and astaxanthin specific yield higher occupied 22.5%, 45% and 46%, respectively.

实验结果表明，在β-紫罗兰酮存在的情况下，由于类胡萝卜素合成受到抑制，海洋红酵母的生物量和虾青素合成量都减少；当平板培养基中β-紫罗兰酮浓度达到370 mg/L时，海洋红酵母的致死率为92.3%；在这种平板培养基上涂布经甲基磺酸乙酯诱变的海洋红酵母，随机筛选200个菌落，结果表明生物量、虾青素体积产率和细胞产率均有所提高的正突变株占18%，生物量、虾青素体积产率和细胞产率的单项指标有所提高的正突变株分别占22.5%、45%和46%。

By the analysis of dive and horizontal plane nonlinear model, the catastrophe model on de dive plane is deduced, and catastrophe stability is analysized. In the horizontal plane, simplified equation is gained by center manifold theory, then draws the conclusion that the stability of limit circle arises from critical point is relate to small difference of move point and critical point.

通过对潜艇垂直面和水平面非线性运动模型进行分析，导出垂直面运动的突变控制模型，并进行了突变稳定性分析；在水平面运动的非线性分析中，通过中心流形定理简化方程，得出临界点处极限环的稳定性与微差占的符号有关的结论，这样在无法或很难得到李亚普诺夫函数的情况下，可以通过这种方法判别临界点的稳定性。

In comparison of the NS gene analysis, changes of the amino acid sequences are lesser than those in the HA gene. The phylogenetic analysis of NS gene is not form different linage between B/Taiwan/3143/97 and B/Taiwan/21706/97, and all isolates were closed to each other.

由HA基因序列的比较中发现，新的分离株的突变位置与B/Taiwan/3143/97大部分都类似，但突变点相较之下数目较少；而在演化树的分析中，新的分离株会与B/Taiwan/3143/97聚集，形成一新的群集。

Previous studies indicate that several mutations alter the voltage dependence of hClC-1 channels. Besides changing the gating property of the Cl- channel, other defects in mutant hClC1 channels may also contribute to the mechanism of myotonia congenita.

前人的研究指出一些与先天性肌肉强直症相关的突变会改变hClC-1通道的电位依赖性，然而除了藉由影响离子通道的门阀开关比例之外，其他由於基因突变造成hClC-1通道的缺失也可能为先天性肌肉强直症的致病机转的成因。

The results revealed that there was nosignificant difference between the symptoms produced by the mutant and thewild type strain by directly injecting the bacteria into mature turnip leaves,however,the mutant was nonpathogenic by inoculations with leaf-clippingand-spraying methods.

应用不同的接种方法对胞外多糖突变体的致病性进行了分析，发现突变体通过注射接种仍能产生与野生型相同症状，但通过喷雾和剪叶接种却完全丧失了致病能力，说明甘蓝黑腐病菌产生的胞外多糖可能在致病过程的早期阶段起作用，但对症状的产生不是必须的。

To make comparison of adaptability of the plastid-less mutant and the wild type strain, we studied their growth characters under different culture conditions, found that the difference of their growth in a medium rich in organic substances are hardly detectable and that the loss of plastid exerts only a weak effect, if any, on the heterotrophic growth.

为比较完全失去质体结构的突变株和野生型的适应力，我们对二者在不同条件下的生长特性进行了研究，结果发现野生型与突变株的生长在有机质丰富的培养液中差别微弱，质体的丢失并不产生显著影响。

In addition, thermostability of high protein concentration was also researched.

从内源荧光的检测结果也可以看出，在相同的变性剂浓度下，突变体的活力损失及三级结构的变化都要快于野生型；在不同的变性剂浓度下，使突变体发生活力损失及三级结构的变化的浓度总是要低于野生型。

In this study, the deletion mutants of SulA with regard to C-terminus were constructed and the interaction with ClpY was analyzed in yeast two-hybrid system.

本实验中，为确认ClpYQ蛋白酶辨认SulA蛋白的区域，建构SulA之C-端不同大小片段缺失的突变蛋白，以酵母菌双杂交系统测试，各个SulA突变蛋白与ClpY之间交互作用的情形，发现ClpY辨识的区域可能位於C-端的高疏水性片段，C-端第20 - 30个胺基酸。

CG strains were isolated from the mango fruits with anthracnose symptoms in different locations on Guangdong and Hainan Province, China. The pathogenicity and sporulation capability of these isolates were compared, and the isolate LCG5 from CATAS, Danzhou City, Hainan Province, China were Chosen for further use in this study for its high virulence on mango fruits and also good sporulation capability.

分别从海口、湛江、两院三地采集到CG菌株进行纯化培养，通过芒果接种实验，对发病率、发病指数及菌株的产孢能力三项测试，最终确定选用接种发病率为100％，病情指数为71.1，菌株产孢能力达1.2×10~6个／ml的两院第5号菌株(缩写为LCG5)，其致病力比较强，产孢能力可以保证了转化体系的成功建立，方便对突变体库的致病和产孢相关突变体的筛选。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。