突变的

In order to understand the drug resistance mechanism of HIV-1 integrase mutually existed for multiple drug-resistant strains to the most potent IN inhibitors diketo acids, three S-1360-resistant HIV-1 strains were selected and molecular docking and molecular dynamics simulations were performed to obtain the inhibitor binding modes.

为了解DKAs引起的多种耐药株共有的耐药性机理，选择3种S-1360引起的IN耐药突变体，用分子对接和分子动力学模拟，研究了野生型和突变型IN与S-1360的结合模式，基于该结合模式探讨了3种耐药突变体所共有的耐药性机理。

Elegans ortholog of the transcription factor Engrailed, controls symmetric and asymmetric division of stem cell-like seam cells.

在ceh-16功能缺失的突变体中，原本应该进行对称分裂的侧线细胞会异常而进行不对称分裂，而且如果在ceh-16突变体中导入人的同源基因 En2能够回复这种异常表型，说明ceh-16的这种调节功能在进化过程中是十分保守的。

The embryonic analysis results indicated that the disruption of oxt gene would lead to the disruption of wg transcription and the expression of engrailed gene, as the wg transcription is dependant on the Engrailed.

进一步结合运用FLP-FRT系统，用缺损GFP蛋白的表达来标记纯合的oxt突变基因克隆，针对眼睛成虫盘的克隆分析结果表明，在oxt突变克隆中，Hh下游的Ci蛋白的表达区域完全消失。

ASR1 is a gene from early auxin-responsive GH3 gene family, and the protein encoded by this gene is overexpressed in the mutant, which results in the following morphological phenotypes including dwarf, epinastic rosette leaves, and restrained lateral root formation, etc. Meanwhile, ASR1 may modify the plant defense signaling molecule salicylic acid in vivo, and therefore asr1 mutant is more susceptible to the infection from the pathogen Pseudomonas syringae.

ASR1属于生长素相关的GH3基因家族，它编码的蛋白质在突变体内过量表达，并造成植株矮化，莲座叶片向下卷曲，侧根发生受到抑制等形态表型的改变，同时，ASR1可能还参与了植物体对抗病信号分子水杨酸的调控或修饰过程，并使asr1突变体对病原菌丁香假单胞杆菌的侵染更敏感。

In fact, any factor which interfere the formation of the hexameric IL-6 receptor complex might inhibit or antagonize the effect of IL-6. These factors include the IL-6 mutant, sIL-6Rα mutant and soluble gp130, as well as their antibodies.

事实上，任何能干扰IL-6信号转导中异六聚体复合物形成的因素都可能抑制或拮抗IL-6的作用，其中包括IL-6的突变体分子、sIL-6Rα的突变体分子和可溶性gp130分子以及它们的抗体。

But it is also difficult to identify a mutant related to Tos17 because there are multiple copies of Tos17 in a mutant and only about 10 percent of mutants are from Tos17 insertion. Theoretically, Ac/DS two-element system is regarded as a best approach for rice insertional mutagenesis.

Tos17是目前应用最为成功的反转录转座子，但多拷贝的插入使突变体的表型鉴定和分子鉴定较为困难，因为只有10%左右的突变性状是由Tos 17插入引起的。

By comparing the amino acids sequences of SCRs of different regulators of complement activation and homologous structure units of DAF in different species, the conservative and nonconservative amino acids in DAF were determined. The possible activity sites were analysed and mutation amino acids sites to construct the mutant library were defined.3. Constructing and evaluating of yeast display mutant library on DAF scaffold.

SCRs多重序列比较及DAF突变位点的选择通过对补体调控蛋白家族成员、不同种属DAF分子的同源结构单位SCR的氨基酸序列进行多重序列比较，确定了DAF分子中的保守性氨基酸和非保守性氨基酸，并根据已有的实验结果，对DAF分子可能活性位点进行了分析，确定了构建酵母细胞表面呈现库的突变氨基酸位点。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

There were location and morphous changing of megakaryocytes in all PV、ET and IMF patients;the incidence rate of JAK2V617F in PV、ETand IMF patients were 80%、26.7%、50% respectively;the mean of leucocytes in patients with JAK2V617F was higher than patiens without JAK2V617F, different stages of reticulin had no impact on the leucocytes in PV and ET patients who were dignosed in the first time.

PV、ET、IMF患者骨髓切片中均有巨核细胞的形态以及定位变化，PV， ET、IMF患者中JAK2V617F的发生率分别为80%、26.7%、50%；有突变患者的白细胞数均值高于无突变患者，初诊的PV、ET患者骨髓中不同纤维增生程度患者的白细胞均值无差别。

To investigate the gene mutation of clinical and neuroelectrophysiological characteristics in Machado-Joseph disease. The gene mutation was detected in 45 patients diagnosed as spinocerebellar ataxia and 30 "healthy relatives". Brain stem evoked potentials, visual evoked potentials and motor conduction velocity and sensory conduction velocity were performed on MJD.

为了解Machado-Joseph病基因突变及临床的神经电生理特点，对16个诊断为遗传性小脑性共济失调家系的45例病人及30例家系的&正常&人作MJD基因突变分析，检出MJD基因的病人行肢体运动及感觉神经传导速度、脑干诱发电位，视觉诱发电位的检查。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。