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Results (1)Multiple copies of pMCLacI/Neo plasmid DNA were intactly integrated in the genomic DNAs of the cell clone.(2) One of lac Ⅰ target genes in the integrated plasmid could be transcribed in the NIH3T3 cells while the other could not.

因此,在这些突变检测系统中,通过一些不表达的中性靶基因所检测到的突变规律是否代表体内一些重要的功能基因的真实情况,目前尚不清楚[1-3]。

This paper aims at detecting bursts of both monotonic and non-monotonic aggregates over multiple windows in data streams.

为了在数据流上检测多个滑动窗口上的单调聚集函数值和非单调聚集函数值的突变,提出了基于分形技术的构建单调搜索空间的突变检测算法。

The character of flower colour and high nicotine conversion inherited steadily,the average nicotine content of their offsprings decreased,while nornicotine content increased significantly.

通过对发现的5株白肋烟B37花色突变株后代的连续观察与研究,结果表明,5株白花突变株当代均为高烟碱转化株;随着世代的增加,白花后代群体花色遗传稳定,高转化率性状更趋于纯合;烟株群体的平均烟碱含量明显下降,降烟碱则明显上升,烟株群体生物碱表现为以降烟碱为主。

For the analysis of the non-kinase activity and the relationship between kinase activity and non-kinase activity, the recombinant full length MLCK was expressed and purified quantifiably with the E.coli expression system. The ATP binding site site-directed mutants of MLCK which lost the kinase activity were acquired.

为了更好地研究MLCK分子的非激酶作用以及激酶与非激酶两者的关系,本实验利用大肠杆菌表达系统大量表达并纯化出完整的MLCK分子,并对其ATP结合位点进行定点突变,获得无激酶活性的MLCK突变体。

Ansochromogenes was transformed by pUC1169 from itself. 8 mutants lost ability to produce nikkomycin were screened from 4068 colonies of Vio〓 Thio〓 with Tn4560 transposition. Southern blotting suggested that Tn4560 was inserted in at least 4 positions of chromosomal DNA in S.

于39℃,MM/Vio条件下培养携带有pUC1169的7100孢子,Tn4560发生转座;筛选到4068个转座菌落,并从中得到8株尼可霉素阻断突变株;对这8株突变株的总DNA进行Southern杂交分析表明,Tn4560至少在4个不同的位点插入到7100的染色体上。

The results of genetic analysis, such as GC content, transition/transversion rate ratios, synonymous substitution rates, indicated that the br fragments with high level of genetic divergence are faced with both purifying selection and bias mutation pressure.

随后的遗传分析,包括GC含量、转换与颠换的比率、同义突变率分析,表明br基因间具有较高的遗传分歧程度,并面临着净化选择和偏倚突变压的双重抑制。

But both 20 years and 2 years old of pu-erh tea extracts show a strong inhibitory effects against 2-AAF. In addition, tannic acid, gallic acid, rutin, umbelliferone, and quercetin exhibited stronger antimutagenicities against PhIP than apigenin; while all of these polyphenols displayed inhibitory effect against 2-AAF.

而比较数种多酚类化合物发现,tannic acid、gallic acid、rutin、umbelliferone和quercetin对於抑制PhIP的抗突变作用比apigenin来的有效,但是apigenin和其他多酚类化合物对於抑制2-AAF的抗突变作用则均具有明显的抑制效果。

It is caused by mutations in dyskerin-encoding genes, telomerase-encoding RNA component genes and reverse transcriptase genes, as well as other uncharacterized genes. There are three inherited forms, including X-linked, autosomal dominant and autosomal recessive inheritance.

先天性角化不良是与编码角化不良蛋白基因、编码端粒酶的RNA组份基因、编码端粒酶的逆转录酶基因突变及其他未确认的致病基因突变引起的基因病,其遗传方式有:X-性联隐性遗传、常染色体显性遗传及常染色体隐性遗传。

In some conditions the fluctuation of stock price is not a continuous process,in order to imitate and analyze the uncontinuous process,a cusp catastrophe model of stock price is set up,in the mean time makes a brief analysis of this model,and finally gives some advices according to the analysis.

针对股票价格变化在某种条件下具有不连续性的问题,对这种不连续的股票价格变化进行了描述与分析。以突变理论为基础,建立了一个描述股票价格短期变化过程的尖点突变模型,并对该模型进行了简要分析。最后,根据分析结果提出了相应的政策建议

BRAF mutations were detected in 13 of the 26 nodal nevi, but in just 1 of the 26 adjacent controls 50% vs.

同时,我们给每个病例都用了与其匹配的邻近淋巴组织作为阴性对照。26例淋巴结色素痣中有13例检测到BRAF基因的突变,邻近对照的淋巴组织中只有1例检测到BRAF的突变

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。