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While doing the RFLP tagging of giant embryogene,the genome changebetween mutants and its original parent,the relationship between RFLP andheteroses in indica,japonica and intermediate rice,the performance of multipleand null allelism in rice varieties,the utilization of null allelic RFLP probes inidentifying indica and japonica rice specifically,the distribution of the nullallelic RFLP probe RG684 in rice cultivars,and the segregations of RFLPmakers in indica/japonica F2 population were also studied.

本实验从经典遗传、分子遗传、育种潜力评估、育种新品系选育等领域,对巨大胚、甜胚乳、暗胚乳三种新型突变体进行了全面系统研究。在对巨大胚进行分子定位过程中,对涉及到的突变体与原始亲本基因组变化,籼、粳、中间型材料的RFLP多态性与杂种优势之间关系,水稻品种的复等位、零等位表现,鉴定籼、粳特异性的零等位RFLP标记,零等位标记RG684在稻种中分布,RFLP标记在籼粳交F2群体中分离等遗传现象也一并进行了分析。

Longitudinal root sections of the wild type and the gls mutant cultured in nutrient solution for 4 days showed that biosynthesis and distribution of amyloplast in the columella cells of gls were normal as compared with the wild type, suggesting that root gravitropism loss in gls mutant is not caused by the defect in amyloplast sedimentation, but possibly by the disturbance of auxin transport and distributation.

4天龄的根系纵切片结果显示:突变体gls根尖结构完整,根尖柱细胞内淀粉粒的生成和分布正常,表明gls突变体根系向地性的缺失不是由于淀粉体的原因,而可能通过影响生长素运输和分布造成。

Data on mutant strains includes detailed information on affected gene, mutant allele and genetic background (DNA origin, gene targeted, host and backcross strain background).

突变系的数据包括对影响的基因、突变等位基因和遗传背景(DNA起源,基因靶向,宿主和回交系背景)的详细的信息。

Carriers of either mutation who were diagnosed with breast cancer before age 55 faced an 18 percent cumulative probability of developing cancer in the opposite breast within 10 years as compared to a 5 percent cumulative probability among women who were mutation-free.

无论是谁的乳腺癌诊断与基因突变携带者55岁之前在10年内面临着一个发展中的百分之18的对面乳腺癌的累积概率为百分之五比一,妇女谁是突变无累积概率。

The problem of nonlinear dynamic buckling of symmetrically laminated, cylindrically orthotropic truncated shells including transverse shear has been studied.

研究了复合材料层合开顶扁球壳的非线性动态屈曲问题。建立了对称层合圆柱正交异性开顶扁球壳考虑横向剪切的非线性振动微分方程,根据突变理论建立了该壳体动态屈曲的突变模型,得到了动态屈曲的临界方

Mutation of the gARID1 binding site in the cwp1 promoter resulted in a decrease of promoter activity and mRNA levels during vegetative growth and encystation, suggesting that the gARID1 binding sites are positive cis-acting elements of the cwp1 gene promoters in both trophozoites and encysting cells. Using cotransfection assay, we also found overexpession of gARID1 can increase luciferase activity and mRNA levels, while mutation of the gARID1 binding sites of the cwp1 promoter resulted loss of this transactivation.

我们也用萤光酵素基因接上cwp1基因启动子,如果将其cwp1启动子上的gARID1结合位置突变,也可以发现萤光酵素和讯息RNA的表现有明显的下;再用cotransfection assay,可发现gARID1会使萤光酵素和讯息RNA表现明显上升,然而如果将其cwp1启动子上的gARID1结合位置突变,这种转活化则消失。

The 4D microscopic analysis revealed that cnx-1 and crt-1 mutations prolong cell-corpse duration time but do not affect the timewhen cells exhibit the death phenotype. This suggests that cnx-1 and crt-1 act in engulfment rather than in execution of apoptosis.

由於在细胞尸体的吞噬机制上,先前已有两条被确认的路径,分别为ced-1, 6, 7 和 ced-2, 5, 10;为了厘清cnx-1和crt-1所作用的路径为何,我们将此两基因分别和ced-1及ced-5两基因做双重突变并分析这些双重突变品系虫体在L1幼虫时期残留的未被吞噬之细胞尸体数量。

Mutants withdefects in epiboly, axis, somite, head, and cardiac and blood systems were identifiedby observing morphological changes in F3 embryos. Additionally, in situhybridization was adopted to identify mutant lines with altered expression patterns ofdpr2, frb35 and insulin.

通过对 F3 代胚胎的表型观察,鉴定了在外包、体轴、体节、头部、心血管系统等表现异常的突变体;通过原位杂交的方法,筛选了 dpr2,frb35 和胰岛素基因表达异常的突变体。

The expression absence of LRRC4 was ascribed to the loss of homozygosity of 7q32-ter in U251 cell lines. Conclusion The expression of LRRC4 gene is absent in glioblastoma cell lines, and it offers the important experiment proof for LRRC4 to act as a new candidate of brain tumor suppressor gene from glioma.

通过对胶质瘤细胞系基因组DNA中LRRC4的ORF序列的PCR扩增和测序分析,发现SF126,SF767,M17细胞中,ORF序列第279位氨基酸的第3个密码子存在同义点突变(3/5),而U251和U87细胞基因组DNA中发生了LRRC4基因的缺失突变(2/5)。

Restriction mechanism for fiber elongation of Lil mutant grown in Held There were abnormities of ultra structure in 9 DPA fibers of Lil mutant whencompared with wild type: thinner cytoplasm, less functional organelles such as Golgi body and endoplasmic reticulum, ect, and more starch grains.

在田间条件下突变体纤维伸长受抑制的原因与野生型比较,突变体开花9d后的纤维细胞在细胞形态学上存在异常:细胞质染色较浅,各种功能性细胞器如高尔基体、内质网等少,有较多的淀粉粒等。

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