突变的

The gene targeted mice are commonly produced by breeding the heterozygous targeted mice following microinjecting the homologous recombinant ES cells into the blastocoele cavity of 3.5dpc embryos at the blastocyst stage to give birth to chimeric mice.

传统基因敲除获得某一突变基因的纯合体动物首先要利用打靶胚胎干细胞获得嵌合体动物；随后用嵌合体动物与野生型个体交配得到突变基因的杂合体动物；最后才能通过杂合体动物之间的交配来生产纯合体动物。

The members with Leber hereditary opˉtic neuropathy have a point mutation in mtDNA and the mutation at np11778is one of mechanisms inducing this disease.PCR technique provides a useful diagnostic aid for LHON.

该LHON家系成员存在线粒体DNA点突变，mtDNA的ND4亚单位第11778核苷酸突变是本病的发病机制之一，PCR-限制性核酸内切酶酶谱分析技术为LHON的诊断提供了依据。

METHODS: We assessed our patients by clinical and electromyographic studies, by intercostal muscle biopsies for in vitro microelectrode analysis of neuromuscular transmission and quantitative electron microscopy EM of 409 end plates, and by mutation analysis, and expression studies of the mutants.

我们通过临床表现及肌电图检查、通过肋间肌活检进行神经肌肉传递的体外微电极分析和对409个运动终板的定量电子显微镜检察，以及运用突变分析和突变体的表达研究对患者进行分析评定。

It was found that there were 36 distinct differences in 184 protein spots. In-gel digestion and liquid chromatography mass spectrometry C LC-MS were made to analyze the protein SSP2801, and Mascot software was applied to identify the protein. The results showed the protein was large subunit of Rubisco, and its content in yellow mutant decreased distinctly, only about 26% of the comparison. Maybe it has relation to etiolation.

切耿其中差异最为明显的SSP2801位点蛋白进行胶内酶切、LC-MS分析，并应用Mascot软件检索鉴定，结果表明该蛋白组分为RUBP羧化酶的大亚基，其在突变体中含量明显减少，仅为对照的26％，表明突变体叶色黄化与RUBP羧化酶大亚基含量减少也有关系。

Genotype BB-representing Arg 669 was well-conserved amino acid with other MC4R subtypes or other species MC4R.

这个突变位于5′-调控区上，对该位点的多态性与肉用性状关系分析，发现突变基因型BB个体的腿肌重显著高于野生型AA个体（P＜0.05）。

One experiment is generation of a lot of chemical-inducible activation-tagging Arabidopsis mutant lines and screen of two salt signaling mutants.

一个部分是通过化学诱导激活标签构建拟南芥突变体库，并从中筛选到两个盐信号突变体；另外一个部分是对拟南芥中的AtCRK3蛋白进行了生化、生理方面的研究。

Using the mutant as male parent crossed with Shuihui-202, 9311, D62B, G46B, 527R, respectively. Their seed setting rate all exceeded 30%. Nevertheless, the rate of seed setting by reversed crossing and mixed-pollinating all were zero, and by self-pollinating was less than 1%.

以突变体为父本分别与202R、9311、D62B、527R、G46B杂交，结实率均达30％以上，而反交结实率均为0，几种花粉混合授粉的结实率也为0，突变体的自交结实率低于1％。

We suggest that it will be used in clinic assay for the detection of HIV-1 drug-resistant mutations as well as other point mutations.

无论在HIV-1耐药性突变还是其它的点突变检测中，该方法都具有较好的临床应用前景。

The ast mutant, which was induced by carbon ion beam, was a single recessive gene mut...

拟南芥ast突变体是由碳离子束诱导产生的与花青苷生物合成有关的突变体，受单隐性核基因控制。

The other mutated genes seemed to be tumor suppressors, the body's natural defense against dangerous genetic mistakes.

其他的基因突变可以抑制肿瘤，是人体对抗因基因突变而产生危险的天然防御机制。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。