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The activities of extracellular amylase, extracellular proteinase, and extracellular cellulase are reduced in panD mutant.

发现突变体的致病力有所下降(接种10天后平均病斑长度为:野生型Xcc 8004 12.29毫米,panD突变体121C07为4.56毫米)。

Objective To decide the base sequences of target position for gene therapy, the K-ras point mutation and its style at codon 12 in human pancreatic cancer cell line Patu 8988 was detected.

目的 检测人胰腺癌细胞株Patu 8988 K-ras基因点突变及其突变方式,明确基因治疗靶点的碱基序列。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式,根据点突变形式设计并合成硫代反义寡核苷酸(K-ras mutation ASODN)作用Patu 8988,通过噻唑蓝比色法检测细胞生长情况;流式细胞术检测K-ras蛋白表达和细胞凋亡;逆转录-聚合酶链反应检测K-ras mRNA表达水平;以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

The major agronomic characters, morphology and the genetic stability of polyembryonic seedling of the mutant line were systematically investigated. The results are as follows. The agronomic characters of IR36-Shuang were obvious different with its parent IR36-4X. The plant height, panicle length, 1000 grain weight and the setting rate of the IR36-Shuang were lower than that of its parent by 35.41%、5.08%、15.72% and 12.39%, respectively.

对该突变株后代的主要农艺性状、多胚苗形态特征和遗传稳定性进行研究表明,突变株系与其原始亲本在主要农艺性状上存在明显差异,前者在株高、穗长、千粒重和结实率等性状上比后者分别下降35.41%、5.08%、15.72%和12.39%。

The mcm10 gene in WJ-1 cells was not reversionally mutated and still maintained the allele in mcm10-1 cells.

序列测定表明,WJ-1细胞中mcm10基因没有发生回复突变,仍维持mcm10-1细胞中mcm10基因的突变。

The mutation rate was about 80% and 10 of the 22 deletion mutations were uncomplete deletion mutations, which had two amplified DNA bands less 509 bp.

在22例缺失突变病例中,有10例为不完全缺失突变即有低于外显子509 bp的扩增产物。

As is known to all, pre-germinal center B cells carry unmutated IgV genes and GC B cells, post-GC B cells carry somatic mutated genes.

目前公认的是,生发中心前B细胞不含体细胞超突变,而生发中心B细胞和生发中心后B细胞表现出体细胞超突变。

By using overlap extension PCR,a P137G site mutant named xylA137 was introduced in the xylose isomerase gene xylA from Thermus thermphilus.

利用重叠延伸法对嗜热细菌的木糖异构酶基因xylA进行体外P137G定点突变,通过酿酒酵母表达载体XM204将突变基因xylA137引入酿酒酵母H158中,得到重组菌株H158-XI137。

But so far as the doped quantity of MoOs less than 5%, the abrupt electrical resistance change of the doped film still can reach 2 orders of magnitude, and commonly cant affect its application, so it is hopeful that temperature of the abrupt electrical resistance change can be reduced to room temperature scope.

不过只要MoO3掺杂量不高于5%时,掺杂薄膜的电阻突变仍可保持2个量级以上,一般不影响其使用功能,因此有望使电阻突变温度降至室温范围。

Experts say the SARS virus certainly will keep mutating, but they do not know whether those mutations will change the seriousness of the disease.

专家们说, SARS病毒肯定将会不断突变,但他们不知道这些突变是否会改变该病的严重性。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。