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Methods mEBP gene was predicatively analyzed by DNASIS software, and the mutations at sites 5 and 18 were induced by PCR site-directed mutagenesis. The mEBP gene was cloned into prokaryotic vector pinpoint Xa-3 and transformed to E.

以亲代EBP基因为基础,结合DNASIS软件理化性质分析确定突变碱基,应用PCR定点诱变技术进行第5、18位谷氨酰胺→赖氨酸的定点突变。

For further investigation on the mutant proteins, we reconstitute wild type and mutant proteins with 〓,〓 and 〓.

为进一步考察半胱氨酸突变后,金属硫蛋白在结构上的变化,我们用〓,〓和〓对野生型及其突变体蛋白进行重组。

The morphological observation has identified 37 mutant lines, the majority ofwhich showed anomalies in axis and somite formation. Ten of them are kept forfurther study.

表型观察法筛选了约 200 个 F2 家族,获得了 37 个突变品系,其中以体轴和体节发育异常的突变体为主,将其中 10 个品系保种,以备进一步研究。

Furthermore, the expression level of the orf19.3926, which locates at the down stream 266 bps from REP3, increased about 3.5 folds in the REP3 rescued strain than that of the wild-type strains and rep3/rep3 mutants in the presence of miconazole.

再者,在药物诱导下,另一个距REP3基因下游处266 个核酸的orf19.3926表现量在rep3/rep3::REP3 突变补救株中比rep3/rep3 突变株及野生株提高了3.5倍。

Result No point mutation of HBD of hPR gene was detected in control group except one with point mutation in trace.

结果 对照组除1例有痕迹量的孕激素受体基因点突变外,其余9例均无点突变存在。

Objective This study was to investigate the roles of TATA or G71R mutation of the undine 5'-diposphate-glucuronosyl transferase 1A1(UGT1A1) gene, A388G mutation of the organic anion transporter 2(OATP2), glucose-6-phosphate dehydrogenase(G-6-PD) deficiency to the development of neonatal hyperbiliidbinemia.

目的 探讨葡萄糖醛酸转移酶UGT1A1 TATA盒或G71R突变、有机阴离子转运因子2(OATP2)A388G突变、葡萄糖-6-磷酸脱氢酶(G-6-PD)缺乏对新生儿高胆红素血症发病的作用。

Anti-cancer and anti-mutation: Many researches prove that the acacatechin in the tea can restrain the cancer cell and the cell mutation.

抗癌及抗突变:许多研究证实茶叶中的儿茶素对癌细胞和细胞突变有抑制作用。

A missense mutation is identified in a pedigree in which the proband's parents are consanguineously married.

基因突变筛查结果发现:在一家系中存在RDS基因错义突变,先证者的双亲为近亲血缘婚配。

Objective To investigate the FECH gene mutation in a Chinese family with erythropoietic protoporphyria, to explore the relationship between gene mutation and clinical manifestations so as to establish a basis for the genetic diagnosis and treatment of erythropoietic protoporphyria.

目的 对一红细胞生成性原卟啉病家系进行基因突变研究,探讨基因突变与临床表现的关系,为进一步开展基因诊断和基因治疗奠定基础。

The study result showed that the system simulation model can simulate the steady and dynamic characteristics of pre-mixed spark ignition gas engine. And the simulation model had certain generalness, which were the same with CNG and LPG.

分别以节气门突变作为激励和负载突变作为扰动,较好地对发动机燃用LPG时的动态工况进行了仿真,并与实测数据进行了对比。

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这一模式非常关注商人的网络信用基础。

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