突变的

Results as bellows: AtSIRT1 was located in Mitochondrial as hSIRT4 of human, and maybe take part in respiration and electron transformation chain, AtSIRT2 was located in nucleolus as hSIRT6 of human, maybe play important role in extend lifespan;mutation in AtSIRT1 leaded to cotyledon of plant turn to yellow and caused short life span. Mutation in AtSIRT2 could make the color of leaf turn to purple and accumulate a lot of anthocyanin;Sirtinol, a inhibitor of SIRT which did not cause the same model of the mutation of AtSIRT1 and AtSIRT2 indicated that the mechanism of Sirtinol was different from other organism;the structure of AtSIRT1 and AtSIRT2 were similar to other known Sir2, which indicated that they maybe have the same function;AtSIRT2 was overexpressed and its activity was detected.

结果表明，1，拟南芥AtSIRT1与人的同源蛋白hSIRT4相同，定位于线粒体，可能参与呼吸作用和电子传递，SIRT2与人的同源蛋白hSIRT6相同，定位于细胞核，可能同它的功能类似，在延缓衰老及调节细胞寿命方面起作用。2，AtSIRT1突变，可引起幼苗和植株的子叶变黄和早衰；AtSIRT2突变，可引起叶片发紫，沉积大量花青素。3，SIRT蛋白的抑制剂Sirtinol不能表型模写AtSIRT1和AtSIRT2突变体，说明Sirtinol在拟南芥中的作用机制不同于其他生物。4，AtSIRT1和AtSIRT2蛋白质结构预测表明与已知的Sir2蛋白相似，揭示其功能的相似性。5，在大肠杆菌中过量表达了其中一个基因(AtSIRT2)，可体外检测其酶学活性，进一步证明其功能。

With western blot analysis and DNA polymerase chain reaction-single strand conformation polymorphism assay,protein product of Rb gene and mutation in exon 5～8 of P_(53) gene were examined in 17 cases of human testicular seminoma.

采用Western印迹和DNA聚合酶链反应－单股构造多态性分析法对17例人睾丸精原细胞瘤组织标本的抗癌基因Rb表达的蛋白产物和P_（53）基因外显子5～8的突变进行检测，发现有3例标本的Rb基因表达的蛋白产物缺失，有4例标本的P_（53）基因有点突变，对照的正常人睾丸组织和视网膜组织均呈Rb基因蛋白产物表达阳性，对照的正常人睾丸组织中未发现P_（53）基因突变。

This work might lead to develop many better methods or means of preventing and controlling dysenteric diarrhea.To elucidate the role of H-NS protein in bacterial physiology, the hns gene insertion and deletion mutants were constructed with RecA and Red recombination system. Then, growth curve and invasion ability of wild strain and deletion mutant were studied.

为了更深入的了解hns基因在细菌生命活动中所起的作用，我们首先利用RecA重组系统构建了痢疾杆菌2457T hns基因的插入突变体；随后，对基于Red重组系统的痢疾杆菌2457T缺失突变体的构建方法进行了摸索并成功地敲除了hns、hdeA、hdeB、yhiE和yhiF 5个基因，研究表明延长同源臂的长度可以明显提高同源重组的效率，缩短构建缺失突变体所需的时间。

The reestablish and error estimation formulas of the non-noise signal under the finitive scale condition is given for the basic filtering operation of high pass, low pass, and band pass respectively. The signal Wavelet analysis softwave developed, which is of some common use, and is served as the basis of the research following it, is also introduced here.

第二章首先建立了突变信号的数学模型；导出了对突变信号进行连续小波分析的尺度截取准则；分别针对突变信号进行高通、低通和带通三种基本滤波操作的情况，给出了在有限尺度分析情况下的非噪部分信号的重建估计和误差估计公式；并介绍了所开发的作为后续工作的基本分析工具、具有一定通用性的信号小波分析软件。

Because homeotic gene mutation and floral organ variation is a direct relationship,therefore the mutant application is the current functional genomics study developmentdirection. We discovered one mutant ah from line 458, which was a line ofthe twin-seedling strain W2555.ln this study, we have investigated the morphogenesisprogress, genetic analysis of mutant traits and molecular tagging of related gene.

目前，由于在水稻中发现的花器官突变体很少，所以对水稻花发育的研究主要是利用双子叶植物MAS-box基因的保守序列设计引物，在水稻基因组或者cDNA文库中筛选与双子叶植物花器官发育相对应的MADS基因，因为同源异型基因的突变与花器官的变异是直接对应的关系，所以突变体的应用是当前功能基因组学的发展方向。

mitochondrial dnais the only inheritant substance except for the nuclear dna in a euˉkaryon cell,which is related to the oxidative phosphorylation of a cell.mitochondrial dna is the important target of carcinogens and is vulnerable because of its structure and function.the mutation of mtdna could reduce the nomal respitation and release large quantities of ros,whichincrease the danger of cancer happening.so,mtdna has been thought to be involved in carcinogenesis and recently more and more studies in this aspect have comeout.in this paˉper,we review the researches for relationship among mtdna mutation and tumors in digestion system,woman system,head and neck,urinary systemand blood system.

线粒体dna（mitochondrial dna，mt dna）是真核细胞中唯一存在的独立于核dna之外的遗传物质，与细胞的氧化磷酸化功能密切相关。mtdna自身的结构和功能特点决定了它是致癌物作用的重要靶点，易受致癌因素的损伤而发生突变。mtdna的突变可削弱正常的呼吸功能，释放高水平的活性氧，进而增加肿瘤发生的危险性，所以mtdna被认为与肿瘤发生有密切关系。近年来，这方面的研究越来越多，本文现就mtdna突变与消化系统肿瘤、妇科肿瘤、头颈部肿瘤、泌尿系统肿瘤等实体瘤以及血液系统恶性肿瘤之间的关系作一简单的综述。

Based on the total potential energy principle, the cusp catastrophic model for instability and failure of deep-buried tunnel surrounding rock is established. Through the observed data of settlement values of typical section vault of deep-buried tunnel and the sudden threshold values of displacement of each monitoring section, the variable values of vault settlement function are obtained, and the stability of deep surrounding rock in tunnel is decided.

运用突变理论探讨了深埋隧道失稳的机制；根据总势能原理，建立了隧道失稳的尖点突变模型，导出了失稳的力学判据条件；并基于深埋隧道典型断面拱顶下沉量现场量测的数据，计算出拱顶下沉函数的各变量值，由各监测断面位移突变阀值来判别深埋隧道围岩的稳定性，分析结果与实际施工围岩情况一致。

For ADRBK2 gene, systematically mutation screening was performed in all 21 exonic and flanking intronic regions through direct sequencing in 48 schizophrenia probands (including 16 Japanese and 32 Chinese probands) randomly selected from our subjects, and the detected variants and eight SNPs reported in the JSNP database were genotyped in 64 trios.

对于ADRBK2基因，在48名先证者(16名日本人和32名中国人)中进行突变筛查，对ADRBK2的21个外显子的所有序列及外显子与内含子邻接区域的部分内含子进行了测序；同时对突变筛查中发现的错义突变及JSNP数据库中的有关SNP，对64个核心家庭(16个日本人核心家庭，48个中国人核心家庭)的成员进行了基因分型。

For ADRBK2 gene, systematically mutation screening was performed in all 21 exonic and flanking intronic regions through direct sequencing in 48 schizophrenia probands (including 16 Japanese and 32 Chinese probands) randomly selected from our subjects, and the detected variants and eight SNPs reported in the JSNP database were genotyped in 64 trios.

对于ADRBK2基因，在48名先证者(16名日本人和 32名中国人)中进行突变筛查，对ADRBK2的21个外显子的所有序列及外显子与内含子邻接区域的部分内含子进行了测序；同时对突变筛查中发现的错义突变及JSNP数据库中的有关SNP，对64个杨L家庭(16个日本人核心家庭，48个中国人核心家庭)的成员进行了基因分型。

A frameshift mutation at base position 217 of exon 1 and a T/C missense mutation at base position 59 of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 of exon 3 was found with similar frequency in three rabbit breeds.

在外显子1的217位检测到由TCT三碱基插入引起的移码突变，在外显子3的59位和3位分别发生了错义突变由T→C和同义突变由T→C。通过计算发现各位点不同的基因型和等位基因频率在3个兔品种中存在较大的差异，位点A、B在长毛兔和肉兔中均有各自的优势基因型和等位基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。