突变的

Phage display of recombinant human lymphotoxin mutation librariesand receptor affinity screeningContraction of conformable large molecule mutation library is pivotal for molecular evolution study in vitro. In order to extend the range of the screening region of amino acid in LT gene, several receptor binding sites of LT were mutated randomly by overlap PCR amplification with the random nucleotide primers, the R46, S106, L130 combined site-directed random mutation library and R46~A52, S106~F110, R46~A52 + S106~F110 region random mutation libraries of rhLT were constructed successfully.

二。重组人淋巴毒素噬菌体库的构建及受体亲和筛选构建适合的大容量生物大分子变异体文库是进行体外分子进化研究的关键，为了扩大LT基因序列中被筛选的氨基酸范围，我们采用含随机核苷酸序列的引物，通过Overlap PCR的方法对LT的受体结合区域进行多点随机突变，分别构建了rhLT R46+S106+L130三点随机突变组合文库和R46～A52、S106～F110、R46～A52+S106～F110区域随机突变体库。

The lifetime of M intermediate of BRE194Q was less than 1.0 s by photomicrography. The lifetime of M intermediates of BRI119T/T121S/A126T and BRI119T/T121S/A126T/E194Q were 3.0 s and 2.0 s, respectively.

显微视频录像技术记录的PVA膜中样品的M态寿命表明，野生型BR的M态寿命最短，单突变体的M态寿命小于1.0 s，三突变体的寿命为3.0 s，四突变体的寿命为2.0 s。

The lifetime of M intermediate of BR(subscript E194Q) was less than 1.0 s by photomicrography. The lifetime of M intermediates of BR(subscript I119T/T121S/A126T) and BR(subscript I191T/T121S/A126T/E194Q) were 3.0s and 2.0s, respectively.

显微视频录像技术记录的PVA膜中样品的M态寿命表明，野生型BR的M态寿命最短，单突变体的M态寿命小于1.0s，三突变体的寿命为3.0s，四突变体的寿命为2.0s。

A mig (mannose insensitive germination) mutantpool, which was created with T-DNA insertion by Kenneth Feldmann, University ofArizona (ecotype Wassilewskija-2, Ws-2), was obtained from the Nottingham ArabidopsisStock Centre, UK. mig3 is one line of the mutant pool, which was thought as aputative mutant for having a high germination rate (about 70%) on MS media containingabout 5-7.5 mM mannose. Lately, the mig3 was found there are some special characteristicsin two French labs by Laurent Signore and Marlire Dieuaide.

本文作者就是以这一突变体为研究对象，开展了有关控制mig3突变体的基因的定位研究研究；并同时开展了在几种糖及其衍生物诱导下的光合作用有关基因表达的研究；这一突变体在开花前后几种糖分含量的调查；开花习性及形态方面的调查。

Systematic infection of mice is used as a model to test the virulence of several mutants. According with their ability to form hyphae, nonfilamentous mutant is avirulent, and hyphae defective mutants have decreased virulence.

用系统感染小鼠模型分析这些突变株的致病能力，同它们形成菌丝的能力相一致，野生菌的致病能力最强，形成菌丝弱的突变株致病能力也减弱，不能形成菌丝的cph1 efg1突变株和crk1则无毒性。

Iba 2002. Both the Arabidopsis fad5 mutant, which lacks an active chloroplast x-9 fatty acid desaturase and accumulates high levels of palmitic acid (16:0), and the fad6 mutant, which lacks an active x-6 fatty acid desaturase and accumulates high levels of palmitoleic acid (16:1) and oleic acid (18:1), have reduced levels of polyunsaturated fatty acids in the chloroplast galactolipids.

两个拟南芥突变体的叶绿体半乳糖脂含有较少的多不饱和脂肪酸：一种是拟南芥fad5突变体，它缺乏有活性的叶绿体的X-9脂肪酸去饱和酶，因而积累了高浓度的棕榈酸（ 16：0 ）；另一种是拟南芥fad6突变体，它缺乏一个有活性的 X - 6脂肪酸脱氢酶因而积累了高浓度的软脂酸（ 16：1 ）和油酸（ 18：1 ）。

The results indicated that the mutant protein promoted the survival of dorsal root ganglion、induced TF-1 prolification and made the normal mice lose weight,decrease appetite and reduce fat index.The weight loss effect was dependant with its administration dosage,ED50 was 150.986?

结果是突变体蛋白能促进鸡胚背根神经节的生长；促进TF-1细胞增殖，MTT测定法表明突变体蛋白与国际参考品相比，比活不低于2.0×106U/mg；使正常小鼠的体重减轻，摄食量减少，脂肪指数下降，并且体重的减轻与突变体蛋白的给药剂量呈现良好的剂量依赖关系，其ED50为：150.986？

Low concentration ryanodine can mimic BDNF to induce growth cone attraction and high dose ryanodine gradient is repulsive to the growth cone.

ryanodine 可以模拟 BDNF 的吸引作用。为进一步研究 Rho GTPases 在轴突转向中的作用，我们将 Cdc42及 RhoA 的显性失活突变基因(dominant negative， DN)及组成性激活突变基因（ constitutively active， CA ）注射到爪蟾胚胎2-4细胞期的一个细胞中，使它们在胚胎神经元中表达，并观察这些突变基因对 BDNF ， LPA ， ryonodine 诱导的轴突转向的不同影响，从而推测 Rho GTPases 是否直接参与并介导轴突导向。

It was typically indirect effect that the following variations induced in the progenies of endosperms implanted by heavy ion beams were discovered. 1 In comparison with control, there were very high differences in biological trait, anti-oxidation enzyme activity, MDA content and protein content, chromosome aberration rate and micro-nucleus rate in M1 generation. 2 Shorter stem variants and purple stem mutants were analyzed by RAPD and AFLP. The analytic results showed that the sequences of their DNA were changed, so those variations were not physiological damage only. And there were differences among M2 generation plants of the same sort of variants or mutants, it was indicated that M2 generation may be in disjunction.

M1代的生物学性状、幼苗的抗氧化酶活性、MDA含量、蛋白质含量和染色体畸变率及微核率等发生了较大的变化。2）对其矮杆变异株和紫色茎杆突变体（M2）做RAPD和AFLP分子鉴定等研究，结果显示了矮杆变异体和紫色茎杆突变体的DNA序列发生了改变，而非仅仅产生生理损伤；同一种变异体或突变体，在M2代单株间也存在着明显的差异，表明它们可能还处于分离状态。

In order to screen out fine pastures adapted to the east seashores of China,Spartina patens mutant lines induced by low energy heavy ion were grown in a simulating the seashore condition outdoors.A comparison of biomass and photosynthetic ability among 10 mutant lines had been carried out,contents of main organic and inorganic nutritients and main heavy metals in above-ground parts of each line were assayed.

为从经低能重离子诱变的狐米草突变系中筛选出适合在滩涂生长、营养丰富的牧草株系，在前文比较了模拟滩涂环境种植的各突变系生物量和光合能力的基础上，本文进一步检测了几种主要有机和无机营养成分含量以及几种主要重金属污染元素在各突变系地上部分的积累量。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。