突变的

The dynamic changes of physiological and biochemical indexes of healthy spikes and inoculation ones of the resistant mutants indicated that PAL activity and content and changing tendency of total phenolate and flavoniods and MDA in the healthy spikes of the mutants were similar to that of their susceptible donors, whereas, for the inoculated spikes, content and changing range of these indexes existed significant difference between two kinds of strains, changing from high-resistant variety (Sumai3 ) to their susceptible donors.

以典型抗扩展品种和突变体供体亲本为抗、感对照，对抗赤霉突变体的健康穗及接种穗的生理生化特性研究结果显示，在健康穗中，抗性突变体的PAL活性、总酚、类黄酮及MDA含量及变化趋势与其感病亲本相一致，但在病原菌侵染后，这些生理生化物质的含量与变化程度与其亲本有了明显的差异，其变化范围介于典型高抗品种和感病亲本之间，变化趋势与亲本或抗性品种一致。

Histological and cellular research on rachis of healthy and inoculated spikes of the mutants at the different stages showed that, structure and developed tendency of cortical sclerenchyma tissue and green tissue and fibrovascular tissue in the rachis were alike between the mutants and their donors at anthesis stage, which also showed no apparent difference in the inoculated spikes'rachis between the mutants and their donors, major difference was that the mutants had less hyphae number than their donors.

对抗赤霉突变体的健康穗及接种穗的穗轴细胞组织学研究结果显示，抗性突变体与其感病亲本的花期健康穗轴在皮层厚壁组织、绿色组织及维管组织结构上基本一致，不同发育时间的变化趋势也一致；接种处理后，抗性突变体与其感病亲本的穗轴组织在各方面的变化趋势相一致，变化量也无明显差异。

The results indicated that whole H-OLE1 gene could complement fad1 mutation in H. polymorpha recipient lacking △9-fatty acid desaturase. However UFA requirement that Saccharomyces cerevisiae ole1 mutant displays was complemented only by ORF of H-OLE1 driven by S.

发现完整的H-OLE1基因可互补缺乏△9-脂肪酸去饱和酶活性的多形汉逊氏酵母营养缺陷型fad1突变体，却不能互补相应的酿酒酵母ole1突变体，而由酿酒酵母GAP表达框架和H-OLE1 ORF组成的嵌合基因可互补上述ole1突变体。

Among Chinese prelingual NSHI patients GJB2 gene mutations may exist in considerable proportion with several different patterns of mutations.

中国人语前NSHI患者存在相当比例的GJB2基因的突变，且有数种不同的突变方式；中国人语前NSHI患者最常见的突变方式为235delC。

All the LOH on 〓 and 〓 was observed in invasive ductal carcino- ma, carcinoma simplex, medulary carcinoma and scirrhous carcino- ma, no deletions at these sites were observed in any invasive lobular carcinoma and others. These results imply an etiological difference.P53 gene is a hot point gene in the occurrence and development of breast, cancer. PCR-SSCP analysis was performed to detect P53 gene point mutation in the region between exon 5 and 8, 5 of 12 (41. 6%) stage I breast cancer patients contain mutation of P53, 3 of 5 patients were accompanied by 〓 deletion. These results suggested that point mutation and allelic loss of P53 gene are two vi- tal genetic events in earlier stage of breast tumorigenesis.

我们还发现，〓和〓位点LOH均分布在乳腺浸润性导管癌、单纯癌、髓样癌及硬癌中，在浸润性小叶癌和某些特殊类型乳腺癌中全部为LOH阴性，上述位点LOH可能与某些组织学类型乳腺癌的发生有关。P53基因是乳腺癌形成过程中的一个热点基因，本研究对12例Ⅰ期乳腺癌组织标本中P53基因热点区域第5、6、7、8外显子点突变进行了测定，发现41.6％（5／12）的病人有P53基因一个或多个点突变，其中3例同时伴有〓位点LOH，表明P53基因点突变和等位基因缺失是发生在乳腺癌形成早期的一个重要遗传学事件。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

It proved that the mutations are generally the transformation or transversion but not the insertion or deletion.

本研究得出的初步结论或推论如下： 1）经过亚硝基胍和PCR诱变法获得的一系列突变体在核苷酸基础上只发生了点突变，由此导致其蛋白质的序列也只是发生了个别氨基酸的突变。

Results In pancreatic cancer patients the positive rate of plasma K - ms gene mutation was 73 %, without false positivity, and higher than that in the pancreatic juice and duodenal juice, but lower than that in the fine - needle aspirates. Plasma K - ras gene mutation was not found in patients with benign pancreatic disease and healthy subjects.

结果 胰腺癌患者血浆K-Fas基因的突变阳性率为73％，无假阳性率，高于胰液、十二指肠液的检测率，低于细针穿刺的检测率；胰腺良性病变患者及健康对照者血浆中未检测到K-ras基因的突变；胰腺癌组K-ras基因突变与非胰腺癌组及健康对照组相比具有显著性差别。

Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料，分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式，研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变，ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变，揭示了突变体对这些基因的巨大影响，反映了它们可能存在相互作用。

In the medium without copper and iron, onlywith ferric citrate , the expression of FDR3 could complement the growth defect of ctrl mutant .CTR1 is a member of high梐ffinity iron transport system .

酵母突变体中铁高亲和系统的成员Ctrl缺失突变，培养基中不供给二价铁和铜，仅以柠檬酸三铁作为唯一的铁源，FDR3基因的表达能够恢复Ctrl突变体在该选择培养基中的表型生长。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。