突变的

Conversely, there is also the same amount of evidence that mutations show no epistasis or antagonistic interactions (each additional mutation has a disproportionally small effect).

相反，也有相同数量的证据表明突变没有上位效应，或者是突变没有拮抗的互作（每增加一个突变，只产生小的、不成比例的影响）。

Six SNPs, which were C→T at the sites of 26, 54 and 99, A→G at the sites of 47and 68, and G→A at the site of 63 in the intron 8 of PRLR gene, were detected by cloning, sequencing and homologous comparison of the homozygote AA, BB and CC.

对AA、BB、CC三种纯合子进行克隆测序和同源序列比较，发现在扩增片段内有6处SNP，都发生在PRLR基因的第8内含子，分别是内含子8第26位、54位和99位的C→T突变，47位和68位的A→G突变，63位的G→A突变。

Some morphological and physiological parameters of Li-1 and TM-1 weremeasured in this experiment. It showed that the contents of chlorophyll in Li-1 washigher than that in TM-1, but leaves areas and photosynthetic rate of Li-1 were lowerthan that of TM-1. The results indicated that lower photosynthetic efficiency and lackof assimilates would decrease the biomass of Li-1, and exhibited other abnormalphenotype such as curled leaves and fiber hypogenesis.

通过对突变体与标准系形态指标和生理指标的测定比较发现，虽然突变体的叶绿素含量高于标准系，但叶面积和光合速率低于标准系，说明生成同化产物的效率较低，这是造成突变体植株生物量较小的直接原因。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BRI119T/T121S/A126T and BRI119T/T121S/A126T/E194Q mutants were blue shifted notably by 11 and 12 nm respectively and the BRE194Q's was weakly red shifted in comparison with WT-BR.

与野生型BR相比较，在水溶液中，单突变体的可见吸收光谱的最大吸收峰发生了轻微红移，三突变体和四突变体的最大吸收峰则分别发生了11.0和12.0 nm的明显蓝移。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BR(subscript I119T/T121S/A126T) and BR(subscript I119T/T121S/A126T/E194Q) mutants were blue shifted notably by 11 and 12 mm respectively and the BR(subscript E194Q)'s was weakly red shifted in comparison with WT-BR.

与野生型BR相比较，在水溶液中，单突变体的可见吸收光谱的最大吸收峰发生了轻微红移，三突变体和四突变体的最大吸收峰则分别发生了11.0和12.0nm的明显蓝移。

Genetic analysis of the mutants revealed that rice GA-deficient mutations are not transmitted as Mendelian traits to the next generation following self-pollination of F1 heterozygous plants, although GA-insensitive mutations are transmitted normally.

遗传分析表明该突变体，水稻遗传缺陷的突变是不转发给下一代孟德尔性状以下自花授粉的 F1的杂合的植物，虽然遗传不敏感突变是正常传送。

Six different deletion and insertion mutants of BBSV-X were constructed and these were inoculated to Chenopodium amaranticolor. The preliminary results show that the sat-RNA could enhance symptom of BBSV-X and its insertion mutants on Chenopodium amaranticolor, but the pathogenicity of the CP deletion mutants was reduced.

构建了BBSV-X基因组6个缺失和插入突变体，将它们的体外转录物单独或与sat-RNA一起混合接种苋色藜，初步实验结果表明，sat-RNA能增强全长BBSV-X基因组及含全长基因组的突变体对苋色藜所致的症状，但减轻CP缺失突变体的致病性。

To screen the sensitive and resistant yeast mutant when they treated with drug, the recombine technology of yeast gene should be applied to obtain the clone expressing the homeotic gene.

通过使用药物对特定的酵母基因突变株进行干预，筛选出对药物敏感或抵抗的突变株，然后利用酵母基因重组技术，使突变株再表达同源的基因，从而获得再表达同一基因的克隆。

However, multiple stable nonsynonymous mutations were detected gradually during 50 passages of 4 serials in embryos with maternal antibody to LG1 strain, and the NS/S ratio was as high as 3.46 among 45 mutated sites.

在2 个传代系列的10 个代次病毒，共出现了29 个位点变异，有义突变与无义突变比值NS/S为1.42 。但在有抗体的鸡胚的传代过程中，发生了多个呈现稳定遗传的有义突变。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。