突变的

Conversely, there is also the same amount of evidence that mutations show no epistasis or antagonistic interactions (each additional mutation has a disproportionally small effect).

相反，也有相同数量的证据表明突变没有上位效应，或者是突变没有拮抗的互作（每增加一个突变，只产生小的、不成比例的影响）。

Six SNPs, which were C→T at the sites of 26, 54 and 99, A→G at the sites of 47and 68, and G→A at the site of 63 in the intron 8 of PRLR gene, were detected by cloning, sequencing and homologous comparison of the homozygote AA, BB and CC.

对AA、BB、CC三种纯合子进行克隆测序和同源序列比较，发现在扩增片段内有6处SNP，都发生在PRLR基因的第8内含子，分别是内含子8第26位、54位和99位的C→T突变，47位和68位的A→G突变，63位的G→A突变。

Some morphological and physiological parameters of Li-1 and TM-1 weremeasured in this experiment. It showed that the contents of chlorophyll in Li-1 washigher than that in TM-1, but leaves areas and photosynthetic rate of Li-1 were lowerthan that of TM-1. The results indicated that lower photosynthetic efficiency and lackof assimilates would decrease the biomass of Li-1, and exhibited other abnormalphenotype such as curled leaves and fiber hypogenesis.

通过对突变体与标准系形态指标和生理指标的测定比较发现，虽然突变体的叶绿素含量高于标准系，但叶面积和光合速率低于标准系，说明生成同化产物的效率较低，这是造成突变体植株生物量较小的直接原因。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BRI119T/T121S/A126T and BRI119T/T121S/A126T/E194Q mutants were blue shifted notably by 11 and 12 nm respectively and the BRE194Q's was weakly red shifted in comparison with WT-BR.

与野生型BR相比较，在水溶液中，单突变体的可见吸收光谱的最大吸收峰发生了轻微红移，三突变体和四突变体的最大吸收峰则分别发生了11.0和12.0 nm的明显蓝移。

The lifetime of M intermediate of the three mutants in PVA films were recorded by employing photomicrography. In distilled water, the visible absorption maximum of BR(subscript I119T/T121S/A126T) and BR(subscript I119T/T121S/A126T/E194Q) mutants were blue shifted notably by 11 and 12 mm respectively and the BR(subscript E194Q)'s was weakly red shifted in comparison with WT-BR.

与野生型BR相比较，在水溶液中，单突变体的可见吸收光谱的最大吸收峰发生了轻微红移，三突变体和四突变体的最大吸收峰则分别发生了11.0和12.0nm的明显蓝移。

Genetic analysis of the mutants revealed that rice GA-deficient mutations are not transmitted as Mendelian traits to the next generation following self-pollination of F1 heterozygous plants, although GA-insensitive mutations are transmitted normally.

遗传分析表明该突变体，水稻遗传缺陷的突变是不转发给下一代孟德尔性状以下自花授粉的 F1的杂合的植物，虽然遗传不敏感突变是正常传送。

Six different deletion and insertion mutants of BBSV-X were constructed and these were inoculated to Chenopodium amaranticolor. The preliminary results show that the sat-RNA could enhance symptom of BBSV-X and its insertion mutants on Chenopodium amaranticolor, but the pathogenicity of the CP deletion mutants was reduced.

构建了BBSV-X基因组6个缺失和插入突变体，将它们的体外转录物单独或与sat-RNA一起混合接种苋色藜，初步实验结果表明，sat-RNA能增强全长BBSV-X基因组及含全长基因组的突变体对苋色藜所致的症状，但减轻CP缺失突变体的致病性。

To screen the sensitive and resistant yeast mutant when they treated with drug, the recombine technology of yeast gene should be applied to obtain the clone expressing the homeotic gene.

通过使用药物对特定的酵母基因突变株进行干预，筛选出对药物敏感或抵抗的突变株，然后利用酵母基因重组技术，使突变株再表达同源的基因，从而获得再表达同一基因的克隆。

However, multiple stable nonsynonymous mutations were detected gradually during 50 passages of 4 serials in embryos with maternal antibody to LG1 strain, and the NS/S ratio was as high as 3.46 among 45 mutated sites.

在2 个传代系列的10 个代次病毒，共出现了29 个位点变异，有义突变与无义突变比值NS/S为1.42 。但在有抗体的鸡胚的传代过程中，发生了多个呈现稳定遗传的有义突变。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。