突变的

Literature Research The etiological factors of POF are not still clear. The changes of sites and numbers in X chromatosome, X; autosome translocations, a forkhead transcription factor gene (FOXL2) mutations, FRAXA premutations and inhibits gene mutations are found in POF patients, so genetic disorders have been thought to be involved. Irradiation, chemotherapy or pelvic operations can damage ovaries and cause ovarian atrophy and failure, so it is thought one factor of POF.

一、文献研究 POF的确切病因目前尚不清楚，从POF患者体内检测到X染色体上位点和数目的变化，X染色体与常染色体间的平衡易位，脆性X基因前突变，FOXL2基因突变，抑制素基因突变等，故认为POF发生与遗传因素有关；由于放射、化疗或盆腔手术等可损伤卵巢，导致卵巢萎缩，出现卵巢衰竭，故也是导致POF的因素之一。

Methods Mimicry and speculation on possible displaced amino acids in proper positions of peptide increased the biological effects of TNFR blocking peptide by compote modeling. The original peptide and its mutant were synthesized. The competitively inhibitory effects on green fluorescence protein-tumor necrosis factor fusion protein with TNFR were compared between original peptide and its mutant (receptor competitively inhibitory binding assays). The inhibitory effects on cytotoxicity were compared between original peptide and its mutant.

通过计算机模建，模拟并推测提高TNFR封闭肽生物学效应需置换的位点和氨基酸；合成原封闭肽和突变肽；受体竞争抑制结合实验比较原肽与突变肽对绿色荧光蛋白－肿瘤坏死因子融合蛋白结合TNFR的竞争抑制效应；细胞毒作用抑制实验比较原肽与突变肽对TNFR的封闭效应。

Drawing on the resource-based view of firm,this paper suggests that an inverted-U shaped relationship exists between market orientation and radical innovations,and offers a theoretic model that focuses on the curvilinearity of the moderating effect of market orientation on the entrepreneurial orientation-radical innovations ...

基于资源与能力理论，在企业家导向对突变创新正向影响的基本命题基础上，分析了不同水平市场导向对企业突变创新的倒U形影响关系，以及市场导向对企业家导向和突变创新间正向关系的倒U形调节作用。

Until now, about 600 types of FBN1 gene mutation have been reported, in which missense mutation and splice site mutation are mainly associated with ectopia lends.

至今已经发现约600种类型的FBN1基因的突变形式，其中与晶状体脱位相关的以错义突变和剪接位点突变为主。

This is the first delineation of an aspartic acid substitution in new site of the al chain causing nonlethal osteogenesis imperfecta.

COL1A1基因突变也是中国人群中成骨不全致病原因之一，现发现的突变属成骨不全一个新的致病基因突变。

The physical experiment on hydraulic fracturing propagation in laboratory showed that the penetration of hydraulic fracture into the salutatory barrier mainly depended on the normal stress difference between salutatory barrier and pay zone.

室内物理模拟实验研究发现，突变体法向应力对水力裂缝能否穿过突变体起着决定性作用，约4MPa的法向应力差可以促使水力裂缝穿透突变体，继续沿原来的方向延伸。

The findings were compared with those from ovarian carcinomas unrelated to endometriosis, atypical endometriosis, and ovarian endome...

结果：起源于内异症的卵巢子宫内膜样癌和卵巢透明细胞癌中各有1例检测到K-ras基因第12密码子发生突变，突变类型为GGT→TGT，即甘氨酸→半胱氨酸，其余标本均未见第12、13密码子突变。p21蛋白在与内异症相关的卵巢肿瘤。。。

Insertional mutagenesis is a method for identifying genes by using the integration of DNA as the mutagen,thereby facilitating the cloning of the mutated gene.

插入突变是一种通过外源DNA整合的方式来获得突变体，并克隆得到对应突变基因的方法。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Main Outcome Measure Incidence of breast cancer in first-degree female relatives of the probands was examined and compared on the basis of proband characteristics and on the basis of variation between families.

通过对患者基因突变筛查和使用统计学回归分析，发现在1394位单侧乳腺癌患者中有73位确定为有害的基因突变，在704位双侧乳腺癌患者中有108位确定为有害的基因突变。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。