突变形成

Chromosomes of the desynaptic variant at metaphase

突变株的花粉母细胞在中期形成8II+8I；K。

The human engrailed homolog En2 functionally substitutes the role of ceh-16 in promoting self-renewal expansion division of seam cells.

此外，ceh- 16突变后还会影响线虫中一个多巴胺神经元的形成，作者还证明这种异常表型也是和侧线细胞的不对称分裂和Wnt信号通路有关。

Our previous studies have shown that ubiquitous overexpression of HmgD caused hemocytes proliferation and the formation of melanotic tumors in the Drosophila larvae. To clarify the mechanism of melanotic tumors appearance,we also generated RT-PCR analysis to test the transcriptional changes of PPAE and Spn27A,which encode the key factors in the phenoloxidasecascade pathway from larvae samples of mid-third instar,late-third instar and prior to pupation relative to wild type flies.Our result showed that in the HmgD overexpression mid-third instar larvae,the expression level.of Spn27A was lower than wild type,otherwise the expression level of PPAE was higher than wild type.It suggests that overexpression of HmgD affects the PO cascade pathway.

本研究组前期工作发现，过量表达HmgD引起果蝇幼虫体内血细胞增生，并出现黑色素瘤，为探讨HmgD过量表达导致了血细胞增生即形成黑色素瘤的分子机制，本研究分别提取三龄中期、后期和相对于野生型化蛹前时刻的幼虫的RNA，首先检测了编码酚氧化酶级联反应途径中的两个关键基因PPAE，Spn27A在突变体中的转录发生变化，结果发现，Spn27A在三龄中期的表达量低于野生型，而PPAE的表达高于野生型，表明过量表达HmgD影响了酚氧化酶级联反应途径，低水平的Spn27A不足以抑制PPAE的活性，活化的PPAE催化了PPO的活性，使其转变成有活性的PO，从而促进黑色素的生成，使果蝇体内产生黑色素瘤。

Moutains of evidences from laboratary and clinical data show that gene abnormality is the major etiological factor of over 40% patients affected with epilepsy.There were also 6 epilepsy genes been cloned successfully, and mutations of over 1000 genes maybe related to epilepsy. These abnormal genes influence many aspects from molecular level to the neuronal plasticity, such as the development of brain,neuronic degeneration, remodeling of neural circuits, energy metabolism, ion channel and so on.These factors would at last affect the formation of epilepsy focus, propagations of epileptic potentials, vulnerability of epilepsy cells.

大量的实验和临床资料提示基因异常是40%以上癫痫患者的病因，有6种常见全身癫痫的基因已被成功克隆，1000种以上的基因突变可能与癫痫的发作有关，这些表达异常的基因分布在脑发育、神经元变性及神经环路重组、能量代谢、离子通道等多个环节，从分子、细胞、神经元可塑性等多个方面影响着癫痫灶的形成、痫性放电的扩布及癫痫细胞的损伤，构成了癫痫的基因机制。

Polycomb group genes Psc and Su2 restrict follicle stem cell self-renewal and extrusion by controlling canonical and noncanonical Wnt signaling.

另外，突变的干细胞从基地膜一侧逐渐突出并脱离出上皮层，在异位不断增长，形成团状肿瘤组织。

With the rich sediments supplied by the Pearl River, under the paleogeography sedimentary background of Baiyun Sag continuously subsiding, with sedimentary sequences related to relative transgressive sea level change ,contrary to regressive eustasy, the Pearl River Deepwater Fan System has specific evolution characteristics, which left the sedimentary record controlled by peripherical significant geological events. The sharp change of deposition rate and lithologic geochemical characteristics about 23.8Ma sequence boundary demonstratesd that sediment source had changed, perhaps related to the uplift of Tibet plateau and spreading of the South China Sea, which led the paleoPearl River drainage to expand to western China plateau , maybe much broader than present.

珠江深水扇系统以珠江为沉积物源、以持续深沉降的白云凹陷为沉积古地理背景、以与全球海退型海平面相反的相对海侵型海平面变化的沉积层序构成了其发育演化的特殊性，并留下了控制沉积的周边重要事件的记录。23.8 Ma等界面沉积速率和岩石地球化学特征均有突变，反映出物源区的变化，可能折射出与青藏高原隆升、南海扩张有关的重要事件，使得古珠江骤然向西部高原拓展，甚至较今天的珠江流域范围还广阔；承载珠江深水扇系统的白云凹陷持续沉降形成了陆坡内盆地，导致21~10.5 Ma BP多个层序深水扇垂向叠置充填的特殊沉积建造；根据60多口井资料建立的相对海平面变化曲线，具有与全球海平面变化相一致的三级旋回和不一致的二级旋回。

With the rich sediments supplied by the Pearl River, under the paleogeography sedimentary background of Baiyun Sag continuously subsiding, with sedimentary sequences related to relative transgressive sea level change ,contrary to regressive eustasy, the Pearl River Deepwater Fan System has specific evolution characteristics, which left the sedimentary record controlled by peripherical significant geological events.

珠江深水扇系统以珠江为沉积物源、以持续深沉降的白云凹陷为沉积古地理背景、以与全球海退型海平面相反的相对海侵型海平面变化的沉积层序构成了其发育演化的特殊性，并留下了控制沉积的周边重要事件的记录。23.8 Ma等界面沉积速率和岩石地球化学特征均有突变，反映出物源区的变化，可能折射出与青藏高原隆升、南海扩张有关的重要事件，使得古珠江骤然向西部高原拓展，甚至较今天的珠江流域范围还广阔；承载珠江深水扇系统的白云凹陷持续沉降形成了陆坡内盆地，导致21~10.5 Ma BP多个层序深水扇垂向叠置充填的特殊沉积建造；根据60多口井资料建立的相对海平面变化曲线，具有与全球海平面变化相一致的三级旋回和不一致的二级旋回。

Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物，我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板，设计了一系列能抑制furin和kexin活性的突变体，采用如下三种方法逐步优化：一、去除Lys片段长链三对二硫键中的一对，用Ser替换Cys，减少可能存在的二硫键错误配对；二、抑制剂的活性中心根据furin底物的专一性逐步改变，即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg，同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用，将P7位Asp替换为Ala；三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

The comprehensive study on the sedimentary features in the early Cretaceous of Tanan Depression showed that the different kind of fault slope belts resulted from syn-sedimentary tectonic movement in the paleo geomorphology controlled the formation and distribution of sedimentary system.

对早白垩世沉积充填特征的综合研究表明，长期活动的同沉积断裂形成的、古地貌上突变的各种断裂坡折带对盆内沉积体系的发育及分布起到重要的控制作用。

Cytological observations of the anther development of pms15-16-2-3 mutant and the wild-type plant showed that the mutant middle layer degenerated later, both the tapetal cell morphology and the tetrads were abnormal, which resulted in few fertile pollen grains formed in the anther.

细胞学观察表明，突变体在花药发育的过程中，中层细胞延迟降解，绒毡层细胞形态分化异常，出现异常的四分体，导致最终只能形成少量的花粉。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。