突变型

Folacin deficiency is likely one of pathogeny of breast cancer, presenting with higher cell proliferation, a lower 〓 period, higher P53 mutational protein expression and lower methyl status of P53 exons 6, 7 and 8, Folacin deficiency also increases apoptosis.

叶酸缺乏可能导致乳腺癌发病率增加，乳腺细胞株HBL-100在叶酸缺乏培养基中，生长显著增快，〓期细胞比例显著降低，凋亡率显著增加，P53突变型蛋白面积比对照组大，mRNA转录增强，P53外显子6、7和8的DNA甲基化程度降低。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

DDRT-PCR method was exploited to study the differential gene expression between immature siliques of Arabidopsis wide-type and ast mutant.

采用DDRT-PCR的策略，分析野生型与突变型植株未成熟角果中基因表达的差异。

Results:Twenty four cases of β thalassemia with CD17 mutant including 3 cases in Shanxi, 20 cases in Gansu and 1 in Xinjiang, were found among 85 probands of β thalassemia.

结果：对该地区的85例β地贫先证者进行了基因分析，检出24例CD17突变型，其中陕西3例、甘肃20例、新疆仅1例，以甘肃地区检出率为最高。

Meanwhile or 24 h later, 316 mg.l-1 sod was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of hela cells were determined. results the production of ros and the apoptosis of hella cells increased as the action of ddp and adm continued within 24 h, but vp-16 failed to produce such effects.

宫颈癌hela细胞经顺铂、足叶乙甙（vp-16）和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入sod对ddp、adm、vp-16诱导hela细胞凋亡及p53、c-fos基因表达的影响。

L^(-1) VP-16 for 6, 12, 24 and 48 h. Meanwhile or 24 h later, 316 mg?L^(-1) SOD was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of Hela cells were determined. Results The production of ROS and the apoptosis of Hella cells increased as the action of DDP and ADM continued within 24 h, hut VP-16 failed to produce such effects.

宫颈癌Hela细胞经顺铂、足叶乙甙(VP-16)和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入SOD对DDP、ADM、VP-16诱导Hela细胞凋亡及p53、c-fos基因表达的影响。

The dose-dependent experiment revealed that Id1 antagonised E47 and Ets1 mediated transcriptional activation in a dose-dependent manner. With pGL3-870 as a template, site-directed mutagenesis of E-box and / or ETS-binding site was introduced into p16 promoter. E47 could not activate the transcription of p16 promoter with E-boxes mutated, and Ets1 could not activate the transcription of p16 promoter with EBS mutated. The synergy effect between E47 and Ets1 would occur only if the E-boxes and EBS were present. These facts demonstrated that E47 and Ets1 proteins were not recruited to the promoter by binding to other cis-elements or through interaction with other protein factors, however, they bound to DNA directly through respective consensus sequences in p16 promoter.

以pGL3-870为模板，构建了E-box或/和EBS点突变的p16启动子报告基因载体，将野生型和突变型的报告载体分别与pI-E47、pI-Etsl以及pI-E47+pI-Ets1共转染，发现E47和Ets1所诱导的转录激活作用依赖于启动子DNA上存在的各自的特异结合序列，二者的协同作用也只有当两个结合位点都存在时才会发生，说明E47和Ets1不是与其它作用元件或其它蛋白质因子相作用而被招募到该启动子的，它们是通过启动子上各自的特异结合位点与DNA直接结合的。

Young adult wild type and mev-1 mutant hermaphrodites were exposed to ENIs the density of ENIs was 1×10^5~l.5×l0^5 ionscm^(-3, and the development period, the lifespan, the broodsize and the germline cell apoptotisis of C. elegans were detected. Results showed that the development periods of two C.

将成年野生型和mev-1突变型线虫及新鲜虫卵持续暴露于空气负离子中（空气负离子剂量1×10^5~1.5×10^5 ionscm^(-3)），观察空气负离子暴露对线虫发育周期、寿命、繁殖性能和性腺细胞凋亡的影响。

Young adult wild type and mev-1 mutant hermaphrodites were exposed to ENIs（the density of ENIs was 1×105~1.5×105ions·cm-3）, and the development period, the lifespan, the broodsize and the germline cell apoptotisis of C. elegans were detected. Results showed that the development periods of two C.

将成年野生型和mev-1突变型线虫及新鲜虫卵持续暴露于空气负离子中（空气负离子剂量1×105~1.5×105ions·cm-3），观察空气负离子暴露对线虫发育周期、寿命、繁殖性能和性腺细胞凋亡的影响。

Elegans were significantly decreased after exposure to ENIs（p.05）; also the brood size and the lifespan of C. elegans were significantly reduced after exposure to ENIs（p.05）. Moreover, after exposure to ENIs, the lifespan of mev-1 mutant hermaphrodites was shorter than that of wild type（p.05）, indicating that mev-1 gene mutant could result in more sensitivity of the mutant hermaphrodites to ENIs exposure than the wild type.

结果表明：空气负离子暴露可显著加速野生型和mev-1突变型线虫发育（p.05），缩短寿命（p.05），与野生型相比，空气负离子暴露对mev-1突变线虫寿命影响更大（p.05）；空气负离子暴露可显著降低线虫后代数量（p.05），原因可能在于空气负离子暴露显著诱导性腺细胞凋亡的增加（p.05）。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。