突变型

The results showed that the changes of the types were not caused by new cell mutation, but by translocation of the different cell types of variegation. The changes of division direction of white cells and green cells resulted that some of the green epidermal cells translocated into the second layer and it pushed furthermore the origin white cells of the second layer into the third layer, so that the variegation altered.

Pollock'突变型异化不是由于细胞突变形成，而是由于质体突变型白色细胞和原型绿色细胞在生长发育过程中相互竞争转轨所致，即白色细胞和原型绿色细胞改变了分裂方向，部分表皮绿色细胞进人第二层，挤压第二层白色细胞转轨进人第三层，导致细胞类型的移位，从而形成异型斑。

Importantly, the changes observed in rat muscle injected with mutant agrin recapitulated the pre- and post-synaptic modifications observed in the patient.

重要的是，大鼠肌肉注射突变型集聚蛋白后产生的变化和在患者中观察到的突出前和突触后变化一致。

Injection of wild-type or mutated agrin into rat soleus muscle induced the formation of nonsynaptic acetylcholine receptor clusters, but the mutant protein specifically destabilized the endogenous neuromuscular junctions.

注射野生型或突变型集聚蛋白到大鼠比目鱼肌中，诱导非突触性乙酰胆碱受体簇的形成，但突变的蛋白破坏了内源性神经肌肉接头的稳定性。

Our study not only proved that calcineurin is the target of nephrotoxic effects of CsA, but also proved that mutant calcineurin is resistant to CsA.

突变型CNA+野生型CNB组和野生型CNA+突变型CNB组的各项改变介于上述两组之间。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母细胞中主要存在Ⅷ突变型，而野生型表达相对较少，成熟后卵母细胞中的Ⅷ突变型蛋白明显增加。

Results The vaccinal vestige, BCO immunization and familial traits were the risk factors of tuberculosis. Frequency of wildness, mutant heterozygote and homozygote of MBP-54 was difference in cases and controls.

MBP-54位点野生型、突变型杂合子、突变型纯合子3种基因型在病例组和对照组中的分布频率不同。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

From the 4687 effective ESTs of Lycoris longituba bud, a total of 2310 different contigs were obtained, 1343 of the 2310 contigs were composed of only one EST, and such contig was also called singlet; 735 contigs were obtained from the 1312 effective ESTs of wild leaf of Lycoris aurea, among which 535 contigs were singlets; 879 contigs were obtained from the 1810 effective ESTs of mutant leaf of Lycoris aurea, among which 574 contigs were singlets.

利用Phred软件对长筒石蒜花苞EST 4992条、忽地笑野生型叶片EST 1606条及忽地笑突变型叶片EST 2132条进行编辑后，分别获得4687条、1312条、1810条有效EST序列，其序列的平均长度分别为545bp、403bp、437bp，序列平均质量数分别为39.28、39.54、40.13；另外通过GC含量分析获得长筒石蒜花苞、忽地笑野生型及突变型叶片EST的GC含量分别39.46、48.33、47.51。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。