穿梭

Methods Cadmium-specific promoter PcadA was obtained by PCR from pPcadA and was ligated to E.coli-B.subtilis shuttle vector pNGFP with gfp being reporter gene ,after which the recombinant plasmid was transformed into 1A751 strain to form the bio-engineered bacteria.

采用聚合酶链式反应从质粒pPcadA上扩增得到重金属镉离子特异性启动子PcadA，以绿色荧光蛋白基因为报告基因，以大肠杆菌-枯草杆菌穿梭载体pNGFP构建了绿色荧光蛋白镉离子诱导表达载体。

Then they set out along the blacktop in the gun-metal light, shuffling through the ash, each the other's world entire.

于是他们便在这泛金属色的冷光中，沿黑色山顶走下，时而穿梭于白蜡树中，彼此即是对方的整个世界。

Jungle River Boat Safari, Tom Sawyer lsland and Bobsled Runs, I think, are all fascinating.

我最欣赏乘小船在森林河道中穿梭探险，游览汤姆莎耶岛和乘坐雪橇。

Here is a view of a terrain considered advanced level because of its bumpiness and the need of going through trees. Can you see there were skiers on the slope?

这是因为它的崎岖不平和需在树木间穿梭，而被视为高难度地带的景象，你能瞧见在滑雪坡上的滑雪客吗？

Tiny butterfly fish and whiting dart among the bubbles I'm spraying from my regulator.

小蝴蝶鱼和石首鱼在我的调节器喷出的泡沫中急速穿梭，这即刻使我的注意力从我在水下呼吸的情况中分散出来。

Their mobility and array of magical abilities make them cagey foes.

他们利用自己的灵活和法术能力在敌人之中穿梭自如。

CaLP's phosphorylation state analysis of different localization showed that when CaLP is overexpressed alone, it is in state of phosphorylation and stays in the cytoplasm; when CaLP is co-transfected with p21, p21 overexpression causes its unphosphorylation and translocated from cytoplasm to nucleus.

对定位于不同部位的CaLP的磷酸化状态的分析表明：当在细胞中单独表达CaLP时，它以磷酸化形式存在，且磷酸化的位点发生在Y139上，CaLP因此定位于细胞质中；当共表达CaLP与p21时，p21的过表达引起CaLP发生去磷酸化，CaLP由细胞质穿梭入细胞核并与p21发生直接相互作用。

Another interesting phenomenon is that when CaLP and p21 are co-transfected, the expression of p21 can stimulate CaLP translocate from cytoplasm to nucleus. By using methods of bioinformatics, point mutation and flow cytometry, we confirmed the importance of Y139 in the nuclear-cytoplasmic trafficking of CaLP.

激光共聚焦实验还发现当p21过表达时能够引起CaLP从细胞质向细胞核的转移，通过生物信息学方法，免疫共沉淀实验和流式细胞分析表明，CaLP的139位酪氨酸残基存在着较高的磷酸化潜力且在CaLP的核质穿梭和蛋白相互作用中起到了重要的作用。

Pedestrian walkways and water components neatly meander throughout the greenery, while the west-east directed canal forms generous lake surface and plaza space.

湿地、水渠与人行步道形成的三条简洁的主线交错穿梭于绿地之间，从地块西部的运河向东部会聚，形成大的湖面与广场空间。

I've been leading my trade caravan across the eastern desert for more than 20 years now.

我已经领导我的商队在东方的沙漠穿梭超过20年了。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。