穿梭

Every once in a while I'll find myself downtown in the middle of the day and I'll see all the drones hustling this way and that with their pinched little waddles, looking at their watches and pouring Starbucks into their faces, and I'll think, my God, I'm the luckiest bastard alive.

每隔一阵子，我会发现自己在大白天身处市中心，看著过往人群像工蜂般嘈嚷穿梭，拥挤摇摆著走路，瞄著手表，吸几口星巴客。这时我会想，天啊，我实在是个最幸运的混蛋。

In the United States he plummeted swiftly from wowing sold-out crowds at Carnegie Hall to sleeping on the Times Square subway shuttle — that is, when he could afford the fare.

而在美国，他迅速从令卡耐基音乐厅爆满轰动的人，而沦落到只能睡在时代广场地铁穿梭巴上--这是他可以负担得起的票价。

In the countryside, yes, there are some restrictions,particularly on using footpaths, that go through farm land. But there is still a huge amount for you to see and do.

的确，在郊外有些地方标注了一些限制，特别是在那些穿梭于农场之间的一些小径，但是这仍意味这你能够去欣赏去体验。

In every Chinese city, we got into the streets, shops, parks, theatres and restaurants.

在每一座中国的城市，我们穿梭于街道、商店、公园、剧院和饭馆之间。

Energetic roving artists will show you the fine sculptures at the City Art Square and around the carnival site.

活力四射的巡游艺人将会载歌载舞，引领你欣赏城市艺坊的优美雕塑以及穿梭嘉年华会场。

As my husband and I walked our wailing baby up and down through the night, and I seriously contemplated going AWOL, I wondered if I was cut out to be a mother at all.

当我和丈夫抱着我们哭个不停的小家伙散步，来来回回的在夜里穿梭的时候，我突然真切地意识到我将擅离职守，我真的生来为做一个母亲吗？

Pyramid shaped dumpling made of glutinous rice wrapped in bamboo or reed leaves;rice dumpling wrapped in reed leaves

生活在不属于我的城市里，忙碌地奔波着，每天穿梭在拥挤地铁中。

The battle smoke and fire in the period of revolution, the snowstorm disaster as well as the earthquake in peaceful time were all dyed with their figures that deserve homage.

革命时期的硝烟炮火，和平时期的雪灾、地震都穿梭着令我们敬佩的士兵的身影，他们是我心目中最崇拜的人。

The reverse side demonstrates the history of Hong Kong through iconic images of people from all walks of life taking in the view of the Victoria Harbour from the Peak : a policeman, a mother and her child in a traditional back carrier, a bird fancier, a worker, a woman with a parasol, a businessman on his mobile phone, and a schoolboy pointing at a hawk.

钞票背面设计犹如穿梭时光隧道E不同时代、阶层的香港人从太平山顶俯瞰现今繁华的维多利亚港，古今对照，相映成趣E尽忠职守的警察、背著婴儿的母亲、弄雀为乐的商家、勤劳的工人、雍容的女士、手执手提电话的上班族和朝气勃勃的小孩子，正是过去百多年来香港人共同建设社会的写照E

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。