穿孔的人

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Collazo is a classy boxer who is well able to fire excellent combinations and function well as a clever counter puncher, but come Saturday I believe those assets will not be sufficient to save Luis from the buzz saw pressure that Hatton will put on him. Frankly, I just don't think that he has the kind of firepower that will disturb Hatton more than a tiny tad. Let us not for one moment deny Collazo his skills, but that boxing ability is never what this fight is going to be about; the real question here is ''who is the tougher man?

可那佐，是个优秀的拳手，他能引爆炸药，同时也能想像聪明的数字穿孔者进行精确计算，，但是有些周6 ，我相信这些能力不足以把刘易斯从哈顿施加给他的压力的难堪中解救出来，坦白的说，我不认为他有能力给予哈顿比小孩还强得打击能力，如果我不考虑考纳佐的地技术，而是这场比赛的能力，真正的问题是谁是最强悍的人？

Moreover, consulting first an expert piercer or a personal doctor is always reasonable as every one is different.

此外，第一次专家咨询穿孔或私人医生总是合理，因为每个人是不同的。

Although many people claim that body piercing does not hurt, the fact is that it is against the law for the piercer to give you an anaesthetic injection before the procedure. They may use creams, wipes or sprays to numb the area that is being pierced, but this is not recommended due to an increased risk of infection.

虽然很多人声称身体穿孔并没有受到伤害，其实这是违反法律为穿孔机，让您一麻醉注射前的程序，他们可能使用面霜，纸巾或喷雾剂，以麻木的领域正在戳穿了，不过，这是不建议由于不断增加的感染的风险。

The practice of piercing can never be held compatible for all people.

穿孔的做法永远不能举行兼容所有的人。

Objective To study the changes of cabin pressure during accidental leakage.

目的 研究载人航天器压力应急状况下尤其是舱壁穿孔时的舱压变化。

In this example,human are involved only when they take output of a program,which is usually a strip of punched paper tape,mount it on the machine's input device,and start up the machine.

在该例中，人要做的仅仅是取出程序--通常是窄窄的穿孔纸带，然后再将其安装到机床的输入设备上，并启动机床。

For men, perforated leather pants, knitwear and shirting laser-cut with the collection's signature dots; t-shirts and hooded sweaters with micro studding, under light weight nylon jackets; rock-infused metallic anoraks and black basics convey the dark inspiration behind the collection with viper green accents on tops and pants.

男装系列以穿孔皮裤、针织衫以及激光割印出这个系列标志型圆点的衬衫最为夺人眼球；小颗铆钉装饰的T恤和连帽衫在薄型尼龙夹克的搭配下显得低调而冷酷；此外，摇滚感十足的金属色短款大衣、黑色基本款，以及如毒蛇般幽绿的上装与裤子传达了此系列所蕴藏的黑夜精神。

Seus corpos foram perfurao armas sem sentido, os seus corpos enterrados no no sabem onde seus corpos por outro mal das larvas foram lentamente tragado pelo nome de um mártir que no têm ah!

他们的身体无意义的被枪炮穿孔、他们的身体被埋在不认识的地方、他们的身体被另人作恶的蛆虫慢慢吞噬，一个个没有名字的烈士啊！

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。