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To prove the H-OLE1 coding for △9-fatty acid desaturase, DNA electroporation system of H. polymorpha was established, and genetic complementation test was conducted.

为验证H-OLE1基因的功能,建立了多形汉逊氏酵母DNA电穿孔实验系统,进行了遗传互补测验。

Coli LLO/OVA as its creating pore activity helping OVA antigen escape from lysosomal vesicle of dendritic cells, and the bacterial content can then be processed by the proteosome and the antigen motifs presented onto MHC class I to CD8+ T cells, initiating stronger cytotoxic T cell immunity.

本实验选用LLO作为免疫佐剂,利用其穿孔素作用促OVA抗原从树突状细胞的吞噬溶酶体进入细胞浆,并经MHC-I类分子递呈给CD8+ T细胞,从而诱导较强抗肿瘤免疫。

Comparative studies of perforation plate structures of vessels in Ranunculaceae.

Title 毛莨科植物导管穿孔板结构的比较观察。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素(osteoprotegerin,OPG),采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中,以电穿孔法转化酵母X-33,用Zeocin 平板筛选重组子,经甲醇诱导表达后, SDS-PAGE 和免疫印迹分析表达产物,由于糖基化不同,表达产物有两种,其相对分子量分别为43kDa和53kDa,表达量约为200mg/L,经Western印迹验证,有较好的抗原性。

The types of apertures include monosulcate, 36 colpate, irregular pantocolpate, tricolporate, 2~4 porate and pantoporate. The ornamentation of exine are spinulose, finely reticulate, coarsely reticulate, granular and stripe-perforated.

花粉的萌发孔主要有单沟、3~6沟、不规则散沟、三孔沟、2~4孔及散孔等6种类型,其外壁纹饰主要包括刺状、细网状、粗网状、颗粒状及条纹穿孔等5种类型。

Combining the previous research on the pollen morphology, the present study indicated that: the plants of Stachyurus shared the common characters in pollen morphology and exine ultrastructure.

结果表明:旌节花属植物花粉形态的共同特征是:花粉粒球形或近球形,三孔沟;外壁具穴状纹饰,穴较细小;外壁内壁分层明显,容易区分,覆盖层光滑,几无附属物,柱状层明显,穿孔较少,不形成半覆盖层。

Methods Thirty-four ears with traumatically perforated tympanic membrane and 57 ears of secretory otitis media with incised tympanic membrane were selected.

以正 负压平衡试验法,检测 34耳外伤性鼓膜穿孔和 5 7耳分泌性中耳炎鼓膜切开后的咽鼓管功能。

A device that collates, merges, or matches sets of punched cards or other documents.

一种整理、合并或比较一组穿孔卡片或其它文档的设备。

Standard grade for turning, drilling, milling, reaming, countersinking of hardened steels, white and grey cast iron light metals, plastics and synthetic materials, Al-Si-Mn-Cu-alloys, glass, ceramics, graphite.

对硬钢、白铸铁、灰铸铁、轻金属、塑料、合成材料、铝-硅-锰-铜合金、玻璃、陶器、石墨等进行车削、钻孔、铣削、铰孔、穿孔等加工的标准牌号。

RKF Standard grade for turning, drilling, milling, reaming, countersinking of hardened steels, white and grey cast iron light metals, plastics and synthetic materials, Al-Si-Mn-Cu-alloys, glass, ceramics, graphite.

对硬钢、白铸铁、灰铸铁、轻金属、塑料、合成材料、铝-硅-锰-铜合金、玻璃、陶器、石墨等进行车削、钻孔、铣削、铰孔、穿孔等加工的标准牌号。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。