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Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

Ultrastructure in the model group as compared with those in the control group was that mitochondrial swelling, unclear and vacuolar degeneration of crista mitochondriales, prosperity of endoplasmic reticulum, and apoptosis of hepatocytes and endothelial cells of hepatic sinusoid.

电子显微镜下可见,模型组大鼠肝细胞线粒体明显肿胀,嵴显示不清,部分呈空泡样变性,肝细胞内质网旺盛,可见肝细胞及肝窦内皮细胞凋亡。

The results of the TEM showed the same respiration epithelium in the inside gill and the gill filament, it is flat, the cytoplast of the epithelium contained of the rough endoplasmic reticulum, vacuole and mitochondrion in rich; the blood-gas barrier is formed of the monolayer epithelium, the endothelial cell and the basilar membrane between the both, following the development the blood-gas barrier attenuate gradually.

电镜结果表明花背蟾蜍外鳃与内鳃具有相同的呼吸上皮,为扁平状,上皮细胞质中富含粗面内质网及空泡和线粒体;气血屏障由由单层上皮细胞,内皮细胞以及二者之间的基底膜构成,随着个体的发育,气血屏障逐渐变薄。

Aspirin or celecoxib can inhibit the growth, urease activity and vacuolating cytotoxin activity of H pylori in vitro significantly.

阿司匹林及塞莱昔布可剂量依赖性的抑制 H pylori 的尿素酶活性及空泡毒素的活性。

AIM: To optimize the electrotransformation condition of Helicobacter pylon and to observe the suppression effect of siRNA on vacuolating cytotoxin A.

目的:利用RNA干扰技术原理,优化小分子干扰RNA电转化幽门螺杆菌的条件,并观察电转化后空泡毒素基因的mRNA表达是否受到抑制。

Based on optical beam deflection,a fiber-optic diagnostic system is proposed to investigate the propagation of a laser-induced plasma shock wave and the oscillation of a cavitation bubble .

实验得到了激光等离子体冲击波传播规律和空泡的动力学特性。

Results the basic CT features of 102 patients with Peripheral lung adenocarcinomas were as follows:vacuole or air bronchogra charging sign was found in which as were seen in 24 cases (80%) in high differential adenocarcinoma and were seen in 10 cases (30.3%) in moderate differential adenocarcinoma but not seen in low differential ones, spiculation sign in 73(71.5%). spiculate protulerance sign in 52 (50.9%). lobulation sign in 79(77.4%), pleural in dentation sign in 65(63.7%), vessel convergenoe sign in 69 cases(67.6%), and calcification inside the lesion was 31 cases. 67 cases had more then three basic CT signs mentioned above.

结果 102例周围型肺腺癌的主要CT分析征象是:空气支气管征或空泡征、高分化腺癌24例(80%)、中分化腺癌10例(30.3%)、低分化腺癌无此征、毛刺征73例、棘突征52例、分叶征79例、胸膜凹陷征65例、血管集束征69例、病灶内钙化31例,其中67例出现上述三种以上征象。

Methods Vacuole incidence of axillary sweat gland was determined by observing the histolomorphological changes of vacuole to evaluate the diaphoretic function.

方法采用组织形态学方法,以大鼠腋窝部皮肤汗腺的空泡发生百分率作为评价发汗强度的指标,观察麻黄汤不同配伍给药后30 min对大鼠的发汗作用。

This subject is experimentally and theoretically studied in the paper and the limit-ing conditions of distribution parameter and drift velocity for upward two-phase flow are proposed.

1引言空泡率代表着两相流动的相分布情况,是决定两相流内部结构的基本参数。

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