稳定化方法

Tricholoma may be a paraphyletic genus two. The collections identified as T. caligatum is a mixture. Some parts of them from France are members of T. matsutake, the other parts from North America are closed to T. bakamatsutake and T. fulvocastaneum. Tricholoma robustum and T. focale form a clade which is obviously a sister group of the stirp Subannulate. The stirp Caligata was defined as a group mainly on the basis of the predominant veil. But from the result of molecular systematic analysis, the presence of veil is not a stable character in the matsutake group. Species having symbiosis with conifers and broad leaf tree are all transformed from those whose host specificity is not strict. The phylogenetic tree based on ITS sequences resembled the tree of NJ algorithm based on cladistic coding. In the phylogenetic tree based on morphylogical characters, T. zangii and T. bakamatsutake form a clade, but it is the result of convergent evolution as suggested by molecular analyses. The distribution area of the matsutake group can be divided into six regions: middle and northern Europe region, Mediterranean region, Hengduan Mount.— Southeastern Tibet region, Northeastern China—Japan—Far-east Russia region, the Atlantic—North America region and the Rocky Mountain region. The abundance center of the matsutake group are Hengduan Mount.—Southeastern Tibet region and the Rocky Mountain region. Hengduan Mount.

松口蘑群的分子系统学研究得出的结果有：由于粗壮口蘑和羽衣口蘑明显和亚环族(Tricholoma，section subannulate)的种类是来自同一个谱系，和其它松口蘑群的种不具有最近共同祖先，因此松口蘑群是一个多系群；松口蘑群和口蘑属其他种类的亲缘关系比和蜜环菌属更接近；梭柄松苞菇和松口蘑群的差距明显小于油黄口蘑(T.flavovirens)和棕灰口蘑；口蘑属是一个多系群；鉴定为欧洲口蘑的标本明显不是一个单系群，其中一部分是松口蘑，另一部分和黄褐口蘑及傻松口蘑(T.bakamatsutake)比较接近；划分松口蘑群的标准是菌环的存在，但从分子系统学的结果来看，菌环的存在与否并非是一个很稳定的性状；和针叶树专性共生及和阔叶树专性共生均由寄主专化性不强的种类转化而来；在形态学系统发育树上青冈口蘑和傻松口蘑被聚为一个分枝，分子系统学的研究结果表明，这是趋同进化的结果；用支序方法编码作出的NJ树的结果更接近ITS序列的结果。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X，脂质体转染HEPG2细胞；潮霉素选择培养稳定表达X基因的HEPG2-X细胞；免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

The stablity of second phases in iron aluminide matrices is analyzed based on the thermodynamic data of binary alloy, which offers the possibility to fortell the chemical reaction in the composite system, and shows that Al〓O〓 is most stable in Fe〓Al matrices.

复合材料强韧化是Fe〓Al合金发展的方向，利用二元化合物热力学数据，提出预测第二相稳定性的计算方法，认为Fe〓Al基合金中，Al〓O〓是最稳定的第二相。

This review focuses on the development and means of the modification of polymeric gene vectors making them have multifunction, i.e. the ability to maintain stability outside of cell, the ability of cell entrance, endosome escape, and the ability of nuclear membrane traversing. The main challenges remain and the future direction of polymeric vectors research are discussed.

本文在分析基因治疗过程的基础上，详述了基因载体应具有的4种基本功能－在细胞外保持稳定的功能、进入细胞的功能、逃离溶酶体的功能和进入细胞核的功能，并详细介绍了有关基因载体功能化修饰的方法和目前国际上的进展情况，总结了高聚物载体目前存在的主要问题及未来的发展方向。

This review focuses on the development and means of the modification of polymeric gene vector making them have multi-function—the ability to maintain stability outside of cell、the ability of cell entrance、the ability of endosome escape and the ability of nuclear membrane traversing, at last, we highlight the main challenges that remain and the future direction of polymeric vectors research.

本文在分析基因治疗过程的基础上，详述了基因载体应具有的四种基本功能——在细胞外保持稳定的功能、进入细胞的功能、逃离溶酶体的功能、进入细胞核的功能，并详细介绍了有关基因载体功能化修饰的方法和目前国际上的进展情况，最后本文总结了高聚物载体目前存在的主要问题及未来的发展方向。

METHODS: Peripheral blood mononuclear cells from 33 AMI patients, 22 unstable angina patients and splenocytes from 35 AMI rats were collected. Cytokine-producing Th cells were monitored by 3-color flow cytometry after stimulated with phorbol myristate acetate and ionomycin.

采用流式细胞分析法对33例AMI病人，22例不稳定心绞痛病人和35例AMI大鼠进行Th细胞胞内干扰素γ和白介素4(IL-4)的动态监测，同时应用RT-PCR方法检测AMI大鼠心肌组织IFN-γ和IL-4以及T细胞表面趋化因子受体CCR3、CCR5和CXCR3 mRNA的表达。

The Faculty of Construction concentrates primarily on the following research areas: Stability of Space Structure Unit, Analysis Methods and Design of Engineering Structure, Application of Compound Material to Civil Engineering, the Application of Finite Characteristic Ratio theory in Soil Mechanics and Application of Local Deformation and Stress Path to Poor Foundation Improvement, The Reliability Analysis and Health Monitoring of Long-span Bridge, and PIRO controlled Rock Blasting.

建设学院在一些技术领域形成了自己的特色和优势，主要研究方向为(1)空间钢结构体系的稳定理论与应用；（2）工程结构的计算分析与设计方法；（3）复合材料在土木工程中应用；（4）橡胶复合材料的力学性能与轮胎结构力学；（5）钢筋混凝土结构现场检测技术；（6）岩土有限性特殊比理论及应用、变形局部化、应力路径问题和软地基处理的理论及应用；（7）大跨度桥梁运行可靠性分析及健康检查。

Analyzing the model, especially the activity of Taihang Mountain piedmont fault, we got such main conclusion:1. Based on sequence stratigraphy, the strata is divided from the selected drills. And the Quaternary stratigraphic framework of Handan city is established, dividing the Quaternary strata into four parts- Qh, Qp3O , Qp2Y and Qp1G.2. With all the selected drill data of Handan city, a drill database is established. A quaternary strata 3D model of Handan city is imitated by GOCAD and ArcGIS, and so are the bottom structural map of each horizon.3. Analyzing the 3D model and the contradistinction section of drills, we got: the stratum of Qh and Qp3 is smooth and flat, with a feature of a higher west, little difference between south and north and undistinctness of faults; there are several depocenters along S-N and N-W in the stratum of Qp2, and the evolution of the stratum is controlled by a couple of ruptures, which means the tectonic movement is active in this period of time.4. Since quaternary, main tectonic movements happened in Qp2 and have been stable in Qp3 and Qh.

获得主要结论如下：1、利用层序地层学的方法将收集的邯郸市钻孔以及论文所依托项目野外施工的钻孔进行了具体的地层划分与对比，建立了邯郸市第四系等时地层格架，将邯郸市第四系划分为全新统、更新统上部欧庄组(Qp3O)、更新统中部杨柳青组(Qp2Y)和更新统下部固安组(Qp1G)。2、利用收集到的邯郸市水文、工勘钻孔以及所依托项目初勘和详勘两个阶段野外施工的钻孔数据，建立钻孔数据库，通过使用三维建模软件GOCAD和地理信息系统软件ArcGIS等进行模拟，得到邯郸市市区部分第四系地层三维可视化模型，并获得各个地层的底部构造图；3、通过对所天生模型和钻孔廊带对比剖面的分析，可以得到：全新世和更新世晚期(Qp3)时期地形整体较平缓，整体上显示西高东低、南北差异不大的格式，断裂显示不明显；更新世中期(Qp2)时期沿南北向和北西向存在几个沉积中心，地层发育受几条断裂的控制，反映了研究区的构造活动在更新世中期较为活跃；4、邯郸市自第四系以来，主要构造运动均发生于更新世中期，到更新世晚期及全新世时期逐渐趋于稳定，进而使更新统上部和全新统地层沉积均一且完好。

In the paper, the factors of the pressure of gas-gathering pipes and high-pressured ammonia water was controlled unstably in the period of Linhuan Coking Limited Liability Company began to product were analyzed, then effective methods about how the coke workshop and chemicals workshop coordinate to control the pressure of gas-gathering pipes and high-pressured ammonia water well in production were made.

文章对临涣焦化股份有限公司投产初期集气管压力与高压氨水压力控制中的不稳定因素进行了分析，并对炼焦车间与化产车间在生产中怎样配合来做好集气管压力与高压氨水压力的控制提出了行之有效的方法。

Based on the geological simplification of the typical sections and dam foundation of Wudu RCC gravity dam ,the 3D geomechanical model test on stability against deep sliding in the condition of natural foundation of this dam is performed.

通过建立武都碾压混凝土重力坝典型坝段坝基的地质概化模型，采用三维地质力学模型试验方法对其天然地基条件下的深层抗滑稳定问题进行系统研究。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。