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Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003~2007年临床分离的220株Pa,对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况,同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration,MIC),筛选出对亚胺培南耐药的铜绿假单胞菌,并分析其对其它抗生素的药物敏感率;采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株,采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因,采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因,用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况;同时对产AmpC酶的Pa(25株,含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Results showed that extraction abilities depends on the hydrophobic of solutes which pK〓 values are similar, e. g. acetic, propionic, butyric and valeric acids, while pK〓 values affect dominantly the extraction abilities of acids with similar hydrophobic, e. g. formic, acetic, chloroacetic, dichloroacetic, and trichloroacetic acids. Meanwhile, diluents and TOA concentration also affect the extraction equilibria of acids through the specific basicity of TOA .

TOA对酸性差别很小的一元羧酸如乙酸、丙酸、丁酸、戊酸的萃取能力主要取决于羧酸的疏水性参数logP;TOA对疏水性参数差别较小的羧酸如甲酸、乙酸、一氯乙酸、二氯乙酸、三氯乙酸的萃取能力主要取决于羧酸的电性参数;稀释剂的种类、TOA的浓度都会影响TOA的表观碱度pH〓,并通过TOA的表观碱度影响羧酸的萃取平衡。

So ,the temperature is quite essential for an engine to produce power .No engines can work well without suitable operating temperatures .If the engine runs too hot ,it may suffer form pre-ignition,while the air-fuel change is ignited prematurely from excessive combustion chamber temperature.Viscosity of the oil circulating in an over heating engine is reduced.Hot oil also forms varnish and carbon deposits may be drawn into the combustion chamber where it increases HC emission.This also causes poor performance and premature wear ,and may even result in engine damage.What is more,the behavior of the metals at excessively high temperature also differs from that at normal temperatures and can produce a condition in which the metal deforms slowly and continuously at a constant stress,If the engine runs too cold, the fuel will not vaporize properly.If liquid fuel reachees the cylinders,it will reduce lubrication by washing the oil from the cylinder walls and diluting the engin oil.This causes a loss ofperformance, an inrease in HC emissions, and premature engine wear.

所以,温度是很有必要的引擎来产生电力,没有引擎,可以工作,如果没有合适的操作温度,如果发动机运行太热,它可能遭受的形式预点火,而空气燃料的改变是点火过早过量燃烧室temperature.viscosity的石油流通的过度加热发动机是reduced.hot油价也构成了清漆和碳存款可能会被拖入燃烧室而增加hc的emission.this也造成业绩不佳和过早磨损,甚至可能导致发动机damage.what更重要的是,行为的研究金属在过高的温度也有不同,在正常温度下,可以达到的一个条件,其中金属变形速度缓慢,不断在恒应力,如果发动机运行太冷了,燃料价格将不会汽化properly.if液体燃料reachees气瓶,将减少润滑洗油从气缸壁和稀释器oil.this造成损失ofperformance , inrease在hc排放,发动机过早磨损。

Zhang Min:"TrumpJet" technology sets the dosing point of wet end chemical additives at the outlet pipe of machine screen of the head-box, and uses Booster pump to extract stock as dilution media, and uses "TrumpJet" mixing to inject chemical additives into Headbox loop, which dexterously uses energy of stock in Headbox pipelines, making a very low power consumption in Booster pump (only requires the energy of speeding up for chemical additives flow), eliminating large volume of chemical additives for fresh water dilution, and chemical additives and stock can be fully mixed within 2 seconds, and delivered to model for shaping at the maximum of chemical additives efficiency.

在数千年的造纸发展史上,传统的湿部化学品的加入和混合一直是利用加后稀释水的方法通过静态在线混合器来完成的,由于湿部化学品的加入量很小,,其流量仅为 0.1~1升/秒甚至更小,而要将如此之小的流量加入到纸机流浆箱300 ~2000 升/秒的工艺系统浆料流体中,同时达到化学品与造纸纤维均匀接触,即使是消耗大量的清水和使用在线混合器也很难达到&完全&且&有效&地混合,于是就又产生了将化学品的加入点,提前到远离流浆箱的工艺位置,这样可以延长混合时间,但人们忽略了在过早加入化学品的同时也带来了化学品的流失、化学品在短循环系统循环产生有害副产物生成浆管悬挂、化学品水解失效等一系列弊病。

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