稀释的

Linear solvation energy relation was firstly introduced in the paper, and the method and step of gaining the special parameters of solvent and solute were introduced in detail, and these special parameters were used to selection and optimization of extractive agent in extractive distillation. The model of predicting activity coefficient at infinite dilution was established by linear solvation energy relation. By the above theory, several candidate solvents were selected to separate C5 fraction, and by experiment of vapor-liquid phase equilibrium at atmosphere pressure, the mixture of N-methyl-pyrrolidone including 4% water was used extractive agent of separating C5 fraction. Then binary vapor-liquid phase equilibrium of the some compound in C5 fraction and N-methyl-pyrolidone were determined at atmosphere pressure and correlated by NRTL equation. Isoprene being objective compound, experiments were operated in the laboratory-scale column; the processes were simulated by RadFrac model in ASPEN PLUS. And by the combination of experiment and simulation, the new process of separation of C5 was established.

本文首先对线性溶剂化能关系进行了分析介绍，也介绍了采用溶剂化能关系得到溶质溶剂特性参数的方法步骤，并将这些参数用于萃取精馏萃取剂的选择与优化；也通过线性溶剂化能关系式建立无限稀释活度系数预测模型，取得了一定的预测精度；将通过上述方法初选的溶剂进行了常压汽液平衡的测定研究，通过实验研究选定含水4%的N-甲基吡咯烷酮溶液做为C5分离的萃取剂，测定了大量N-甲基吡咯烷酮与C5组分的二元平衡数据，并进行关联计算；以异戊二烯为目标产物在实验室规模的精馏塔内进行了C5分离的实验研究，取得大量塔内数据；通过选用合适的热力学模型，采用ASPEN中RadFrac模块对分离过程进行模拟研究，通过实验与模拟相结合，建立了C5分离的新工艺，为将来的工业化打下了坚实的基础。

Antibacterial function of body wall was conducted by the following way: microorganism was directly resisted through the change of cuticle, such as incrassation of up-cuticle protuberance and increasing length of micro-floccus in the up-cuticle dissociation surface; exudation function of large granular orthochromatic mucous cells and reticulation mucous cells in the epidermis could be enhanced when the Eisenia fetida was induced by E. coli. Moreover, the mucus that contained compound substance of mucopolysaccharide and protein could make the bactericidal function or dilute the microorganism on the surface of earthworm, which could protect earthworm from invasion of microorganism; the content of neutral mucopolysaccharide of antibacterial function could be increased according with the exudation increment of small granular proteinaceous cells in the epidermis, which could resist bacteria invading into the epidermis of earthworm.

结果表明：蚯蚓的体壁主要是通过以下途径来抗细菌的侵袭：一是通过角质层的变化(如上胶层突起的增厚、上角层游离面中微绒毛的变长等)直接抵抗微生物的入侵；二是增加表皮中大颗粒粘液细胞和网状细胞的分泌功能，分泌出大量含有粘多糖蛋白液体复合物的粘液，在蚯蚓体表对细菌进行稀释或者起到直接杀菌的作用；三是通过增加小颗粒蛋白细胞的分泌作用，在蚯蚓的表皮中分泌出更多的抵抗疾病的中性粘多糖，从而抵抗进入蚯蚓体表内的细菌。

Result］ It could be concluded through analyzing the model that both the mass uncertainties of quinoline phosphomolybdate precipitates and tested samples were from the resolving power and precision of balance and its maximum tolerance in assay certificate; the uncertainty brought in by volumetric flask was from the permissible error of 250 ml volumetric flask, the operation of increasing the volume to scale of volumetric flask while diluting solution and the difference between experimental temperature and the temperature at the time when the volumetric flask was calibrated; the uncertainty brought in by pipette was from the permissible error of itself, the operation of increasing the volume to intake scale while taking solution and the difference between experimental temperature and the temperature at the time when the pipette was calibrated.

结果］磷钼酸喹啉沉淀和试样质量的不确定度均来自天平的分辨力、精确度、鉴定证书上天平的最大允差，容量瓶引入的不确定度来自250 ml容量瓶本身的允许误差、稀释溶液时将体积增加到容量瓶刻度的操作和试验温度与容量瓶校准时温度的差异，吸量管引入的不确定度来自于吸量管本身的允许误差、吸取溶液时将体积增加到吸取刻度时的操作和试验温度与吸量管校准时温度的差异。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

The results indicated that apple juice mingled with a certain amount of pear juice was characterized by great changes in pH, 295 nm absorbance, total sugar/reduced sugar and optical rotation, and that these 4 indexes can be used .

从相同稀释倍数下的梨汁以不同体积掺入苹果汁后的pH变化，295nm处的吸光度的变化，总糖和还原糖的变化，旋光度的变化以及糖的薄层层析和感官评定等16个方面进行了探讨。

Employing high-dilute method and 5-fold excess amine prepared two new "two-armed" acyclic diamine intermediates I_a and I_b, which are also intermediates of synthesizing microcyclic compounds, and eight aromatic Schiff bases, II_, were synthesized from reaction of I_a or I_b condensing with different aromatic aldehydes. Their composition and structure were characterized by Elemental analysis, IR, UV-Vis, LIF,~1HNMR and MS. The hydrogen bond interactions and MS fragmental patterns were also discussed.3. Several novel transition metal complexes using II_a as ligand were synthesized and identified by Elemental analysis, IR, UV-Vis,~1HNMR and molar conductivity.

1，采用高度稀释及胺过量五倍的方法合成了两个具有吡啶双酰胺臂的非环二胺Ⅰ_a和Ⅰ_b，并以它们为中间体进一步合成了八个芳香席夫碱Ⅱ_a-Ⅱ_h，通过元素分析及各种波谱测试鉴定了它们的组成与结构，对化合物的UV-Vis、LIF、IR、~1HNMR及MS进行了较为详细的讨论，还探讨了席夫碱的氢键相互作用及质谱裂解方式； 2，以席夫碱Ⅱ_a为配体合成了几个新型的过渡金属配合物，并通过元素分析、UV-Vis、IR、~1HNMR、摩尔电导及DTA—TG对其进行了表征，讨论了化合物的配位情况及配体在配位中的脱质子行为。

Greenhouse pot tests showed that treatments with GJ23,NJ08 and NJ28 have greatly inhibited gall formation on tomato roots.

用GJ23，NJ08和NJ218的菌悬液进行的温室盆钵实验，结果表明不同的浓度处理均对番茄上南方根结线虫的根结形成有显著的抑制作用，其中2倍菌液处理的平均根结数比对照分别少51.4%、62.5%和54.5%；它们还对番茄的生长有明显促进作用，2倍稀释菌液处理组的番茄冠重分别比对照高39.8%、32.0%和60.5%。

Test Preparation Transfer about 2 g of the substance under test, accurately weighed, to a glass-stoppered, 250-mL conical flask, add 20 mL of a mixture of equal volumes of nitric acid and sulfuric acid, attach a suitable condenser, reflux the mixture for 1 hour, cool, cautiously dilute with water, and boil until fumes of nitrous acid no longer are noticeable.

Test准备 Transfer大约2 g在测试之下的物质，准确地被称，对玻璃防止， 250ml conical烧瓶，增加硝酸的相等的容量混合物的20 mL，并且硫酸，附有一适当的 condenser，循环加热混合物1个小时，凉快，慎重地稀释与直到亚硝酸发烟的水和煮沸 acid不再是引人注目的。

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞，加入莫能霉素，用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间，比较其胞内表达白细胞介素－2(IL-2)/白细胞介素－4(IL-4)的细胞百分率，确定最适诱生条件；用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验，确认McAb、bio-SAM IgG和HRP-SA的最适浓度，根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

High-resolution 〓H-NMR spectra were used to confirm the formation of the ICs, and XPS spectra were used to corroborate the formation of S-Au bonds. The significantly improved"reversible"voltammetric responses and the reproducible surface coverages of (2.4±0.2)×10〓 and (2.2±0.2)×10〓 mol/cm〓 obtained for SAMs of β-CD/C〓VC〓SH and α-CD/C〓VC〓SH, respectively, indicate that the intercalated CD can effectively dilute the surface viologen groups and weaken the intermolecular interaction, giving a wellordered and viologen-containing SAM with a determinate surface coverage.

高分辨〓H-NMR谱确证了预期包络物的形成；XPS谱确证了包络物电活性自组膜和S-Au共价键的形成；明显改善的&可逆&伏安响应和重现的紫精基团表面覆盖度[对β和α分别为(2.4±0.1)×10〓和(2.2±0.1)×10〓mol/cm〓]，表明嵌入的环糊精可有效地&稀释&紫精基团，在很大程度上减弱它们之间的相互作用，形成了完备的和有确定紫精覆盖度的包络物电活性自组膜。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。