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稀释液

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to choose right dilution; to strictly master the formulation of skin test and the technique of intracutaneous injection; to fix the best check time; to set the standard of assessing the result of skin test; to strictly master the injection techniqu, e of tetanus antitoxin.

选择正确的稀释液,严格掌握皮试液配制方法及皮内注射方法,规定皮试结果最佳观察时间,规范皮试结果判断标准,严格掌握tat注射原则。

Methods Rabbits were administered by intravaginal amoxicillin and metronidazole solution, then the experimental vaginitis model were established by E. coli and Staphylococcus aureus, were treated with lactobacilli lysates, lactobacilli lysates in lower concentration, lactobacillus fermented broth, the probiotic lactobacillus and N. S for 7 days respectively. The variety in vaginal microecosystem were analysed and investigated.

用阿莫西林和甲硝唑接种家兔建立脱污染模型,再用大肠埃希菌和金黄色葡萄球菌的混合液接种,建立实验性感染模型后,随机分为5组,分别用乳杆菌裂解物原液、裂解物稀释液、发酵上清液、乳杆菌活菌制剂及生理盐水进行治疗,分析和考察阴道菌群变化。

2 Treatment method is less than a month treat a little patient to use method of massage drop medicine first, treat 1~2 week, 2~3min is massaged before daily drop medicine, be more than the little patient of a month, if drop medicine treats 1~2 week to did not see,improve to still have apparently weep the person that reach secretion, applied medicaments pressurization rinses lachrymal path, use pinhead of vein of skin of tube head of nylon of self-restrained and different type to grind blunt, make rinse a needle continuously, groovy operation, after syringe needle inserts lachrymal canaliculus, nylon the canal is secured with adhesive plaster at the head, undertake rinsing, 1 / D, with oxygen fluorine to lotion Sha Xing adds loose diluent of rice of ground a place of strategic importance, pressurization rinses 3~4 second, invalid still, undertake probing of groovy tear path is operated.

我科自2000年1月至2006年12月,共治疗新生儿泪囊炎178例、230眼,疗效满足,报告分析如下。1资料和方法1.1一般资料新生儿泪囊炎患儿168例、230眼,男102例,女66例,年龄:2d~3个月,均为本院眼科门诊确诊的新生儿泪囊炎患儿。1.2治疗方法小于1个月的首次就治患儿采用按摩滴药法,治疗1~2周,每日滴药前按摩2~3min,大于1个月的患儿,如滴药治疗1~2周未见明显好转仍有流泪及分泌物者,应用药物加压冲洗泪道,采用自制不同型号尼龙管头皮静脉针头磨钝,制成直的冲洗针,常规操作,针头插入泪小管后,将尼龙管用胶布固定于头部,进行冲洗,1次/d,冲洗液用氧氟沙星加地塞米松稀释液,加压冲洗3~4次,仍然无效的,进行常规泪道探通术操作。1.3治疗标准无流泪、无分泌物、泪道冲洗畅通,反之则无效,结果168例,230眼,治愈166例,228眼,其中按摩治疗49例,冲洗86眼,探通31眼。。。

Methods ①Using the method of physiology and biochemical to analysis the original liquefier.

方法①对原装稀释液进行理化分析

The results showed that the bacteria B7 came into the logarithmic growth phase for 5 h culture. Both the fermentation liquid and its filtration could inhibit C. colhounii effectively, and the fermentation of dilution l0 times also showed significant differences compared with the control. The inhibiting effect of its fermentation media filtration was even more significant in the optimizing test, with the maximal inhibition rate of 100%. The best fermentation condition was 0.5% glucose, 0.1% ammonium muriate, without extra mineral, pH 5.4, temperature 28℃. Based on the morphological properties and 16S rRNA sequence, the bacterium B7 was identified as the Bacillus subtilis.

结果表明,该菌体在培养了5h后即进入对数生长期;B7细菌的发酵液原液和无菌滤液均能抑制病原菌的生长,其中发酵液原液的10^3稀释液的抑菌效果仍与对照之间存在显著的差异;发酵条件优化试验中发现该细菌的无菌滤液最大抑菌率达100%,最佳发酵条件为葡萄糖0.5%、氯化钱0.1%、不加无机盐、pH5.4,温度28℃;结合形态学特征和16S rRNA的序列分析,将该菌株鉴定为枯草芽孢杆菌。

Methods The study was carried out in laboratory and field in Guangzhou from September to November in 2007. The Mosq-ovitrap in tested group with yeast-generated CO2, Panicum maximum infusion, tyre infusion, L-lactic acid dilution, or darken surface and that in control group with tap water were set at intervals of 20 cm, respectively.

研究在实验室和现场分别进行,实验组诱蚊诱卵器中分别添加酵母发酵产生的CO2、大黍叶浸出液、轮胎浸出液、乳酸稀释液以及改变杯身的颜色,对照组加过夜自来水,布放5 d,计算捕获的白纹伊蚊数量和产卵的数量。

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58%(n=6) and 5. 69%(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体,羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法,该系统的灵敏度为0.412ng GH/ml(n=6),组内变异系数为2.58%(n=6),组间变异系数为5.69%(n=6),斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行,金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行,表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性,灵敏度达到了测试血清样品中GH的水平。

Use method:┢ of wait very hot Qun returns graceful Fu? to take diluent 1 bottle of handstand are pensile, groovy disinfection, insert infusion implement, shut switch A, asepsis injector join is noted - inspiratory connect is backward air of the infuse inside infusion bottle, after amounting to constant pressure, shut switch B.

使用方法:①固体药品的溶解:取稀释液1瓶倒立悬挂,常规消毒,插入输液器,关闭开关A,将无菌注射器连接注-吸气接头后向输液瓶内注入空气,达一定压力后,关闭开关B。

Pipet 1 mL of the final dilution onto each of two sterile petri dishes.

移取1mL最终稀释液到1个无菌平皿,共做两个平皿的平行样。

The rabbits in the experimental group were treated externally with the extraction of Plumbago zeylanica Linn in 50% of ethanol, twice a day for 15 days. The sodium chloride was administrated into rabbits in the control group.

给药组家兔右前肢骨折处外用白花丹全草浸膏的体积分数为0.5的乙醇稀释液,对照组给予生理盐水,2次/d,连续15 d。

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