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The single cell was isolated from the massive cultivation according to a semi-quantum approaching technique and then lysed before mRNA separation.

采用逐级稀释、半量逼近的方法,从培养体系中分离单个细胞,对其进行裂解处理。

The single cell was isolated from the massive cultivation according to semi-quantum approaching technique and then was lysed before mRNA separation.

采用逐级稀释、半量逼近的方法,从培养体系中分离单个细胞,对其进行裂解处理。

BALB/C mice were immunized with the purified PrV of MIN A isolate,mouse spleen cell were fused with myelomas NSo.Culture supernatants of hybridomas were screened by indirect ELISA and immobilization ELISA,positive colonies were cloned three times by limited dilution.

用纯化的伪狂犬病病毒免疫BALB/C小鼠,取脾细胞与NS_0骨髓瘤细胞融合,经间接ELISA和固相化ELISA筛选,3次有限稀释法克隆,获得了1株能稳定分泌抗伪狂犬病病毒单克隆抗体杂交瘤细胞株,命名为OH_6。

Reached slightly higher iodine delivery rates with a 300 mg l/ml iopromide solution than with 370 mgI/ml or iopamidol 370 mgl/ml.10The advantage of less concentrated (e.g. 300 mgl/ml) over more concentrated low osmolar contrast media in respect of injection and iodine delivery rates at room temperature and at 37°C was confirmed.11,12 In vivo, a slightly slower intravenous injection of concentrated contrast media may be compensated or even overcompensated because a higher concentration may be maintained after dilution by a given rate of blood flow.

到达递送以这 300 毫克 l│毫升 iopromide 解决评估的些微比较高的碘超过与 370 mgI│毫升或 iopamidol 370 mgl│毫升。10个比较不集中的(举例来说 300 mgl│毫升)结束更集中的低 osmolar 差别媒体的利益在注入和碘递送率方面在室温和 37 °C 被确认了。11,12在活泼地,一个集中差别媒体的些微比较慢静脉内注入可能被偿还或者平坦的 overcompensated 因为较高的集中可能在稀释之后被血流程的给定比率所维护。

Cell suspension was prepared and diluted when diameters of the fourth passaged cell spheres were 2μm.

当第4代细胞大多数克隆球直径约为2μm时,利用有限稀释法进行单细胞克隆培养。

Two 0.5-ml volumes of an isobutanol internal standard (10 mg/ml water; pipette 12.4 ml of isobutanol and dilute to 1 liter with water) are pipetted into two different 2-dram (7.4-ml) shell vials, one market "known."

将两份0.5毫升的异丁醇内标物(浓度为10mg/ml水,其配制法为:用吸量管吸取12.4ml 异丁醇用水稀释至1L ),用吸量管分别吸入两个不同的2-打兰(7.4毫升)的壳型小瓶中,一个标为&已知&,另一个标为未

Two 0.5-ml volumes of an isobutanol internal standard (10 mg/ml water; pipette 12.4 ml of isobutanol and dilute to 1 liter with water) are pipetted into two different 2-dram (7.4-ml) shell vials, one market "known."and the other "unknown."

将两份0.5毫升的异丁醇内标物(浓度为10mg/ml水,其配制法为:用吸量管吸取12.4ml 异丁醇用水稀释至1L ),用吸量管分别吸入两个不同的2-打兰(7.4毫升)的壳型小瓶中,一个标为&已知&,另一个标为&未知&。

Store up to five of seven possible programs—perform sequential pipetting, sequential dispensing, and serial dilution.

可储存7个可能程序中的5个程序—可连续吸液、连续分发和连续稀释

An intuitive user interface allows easy set-up and use of liquid handling functions for pipetting, mixing, multiple dispensing, serial dilutions and reverse pipetting.

一个直观的用户界面可以轻松地设置和使用的液体处理功能pipetting ,混合,多配药,串行稀释和扭转

Apart from tears,only time could wear everything away,while feeling is being precessed by time,conflicts will be reconciled as the time goes by,just like a cup of tea that is being continously diluted.

能冲刷一切的除了眼泪,就是时间,以时间来推移感情,时间越长,冲突越淡,仿佛不断稀释的茶。

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